FB2025_01 , released February 20, 2025
Allele: Dmel\KhcUAS.cFa
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General Information
Symbol
Dmel\KhcUAS.cFa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Fuger
FlyBase ID
FBal0281727
Feature type
allele
Associated gene
Dmel\Khc
Associated Insertion(s)
Carried in Construct
M{UAS-Khc.F}
Key Links
Transgenic product class
cDNA
(Füger et al., 2012)
wild_type
(Füger et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Füger et al., 2012)
wild_type
(Füger et al., 2012)
Mutagen
in vitro construct
(Füger et al., 2012)
Progenitor genotype
Carried in construct
M{UAS-Khc.F}
Cytology
Description

UAS regulatory sequences drive expression of the Khc SD02406 cDNA.

(Füger et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Khc
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of KhcScer\UAS.cFa under the control of Scer\GAL4D42 at 18[o]C results in a minimal but significant reduction in lifespan.

      Expression of KhcScer\UAS.cFa under the control of Scer\GAL4D42 at either 25 or 29[o]C does not result in any obvious defects in larval locomotion.

      16 day old adults in which KhcScer\UAS.cFa is expressed under the control of Scer\GAL4elav.PU after eclosion (the temperature sensitive Scer\GAL80ts.αTub84B allele is used to limit expression prior to eclosion) show normal climbing ability.

      A strong reduction in both anterograde and retrograde flux of mitochondria along the axons is seen in larvae co-expressing both KhcScer\UAS.cFa and KhcN262S.Scer\UAS under the control of Scer\GAL4D42.

      Quantal content at the neuromuscular junction is significantly reduced in larvae co-expressing both KhcScer\UAS.cFa and KhcN262S.Scer\UAS under the control of Scer\GAL4D42.

      (Füger et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      KhcUAS.cFa/Scer\GAL4Toll-6-D42 partially rescues KhcN262S.UAS

      (Füger et al., 2012)
      Comments

      Co-expression of KhcScer\UAS.cFa ameliorates the paralysis phenotype seen in larvae expressing KhcN262S.Scer\UAS under the control of Scer\GAL4D42 at either 25 or 29[o]C , with the larvae displaying a tail-flipping phenotype at a stage where larvae expressing only KhcN262S.Scer\UAS under the control of Scer\GAL4D42 can only move their heads.

      (Füger et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      KhcScer\UAS.cFa
      KhcUAS.cFa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Fuger
      Secondary FlyBase IDs
        References (1)