FlyBase Allele Report: Hsap\MAPT[UAS.wt]

General Information

Symbol

Hsap\MAPT^UAS.wt

Species

H. sapiens

Name

FlyBase ID

FBal0281833

Feature type

allele

Associated gene

Hsap\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-Tau.wt}

Also Known As

UAS-hTau, UAS-Tau, UAS-Tau^wt

Key Links

Transgenic product class

cDNA

(Chatterjee et al., 2009)

wild_type

(Chatterjee et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Chatterjee et al., 2009)

wild_type

(Chatterjee et al., 2009)

Mutagen

in vitro construct

(Chatterjee et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Tau.wt}

Cytology

Description

UASt regulates expression of a cDNA encoding wild type Hsap\MAPT 4R.

(Chatterjee et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\MAPT

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of tauopathy

CEA

(Li et al., 2024, Li et al., 2023, Subramanian et al., 2021, Lee et al., 2020, Lee et al., 2020, Panchal and Tiwari, 2020, Wang et al., 2020, Frenkel-Pinter et al., 2016, Chatterjee et al., 2009)

CEC

(Yang et al., 2024)

model of Alzheimer's disease

CEA

(Frenkel-Pinter et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of tauopathy

is exacerbated by sgg^UAS.cSa

(Chatterjee et al., 2009)

is exacerbated by par-1^UAS.cSa

(Chatterjee et al., 2009)

is ameliorated by AMPKα^UAS.cMa

(Li et al., 2024)

is exacerbated by mnb^UAS.Tag:FLAG

(Lee et al., 2020)

is exacerbated by Mitf^{2KQ.UAS.Tag:FLAG}

(Wang et al., 2020)

is exacerbated by Mitf^HMS00151

(Wang et al., 2020)

is ameliorated by Mitf^UAS.Tag:FLAG

(Wang et al., 2020)

is ameliorated by Gcn5^RNAi.UAS

(Wang et al., 2020)

is ameliorated by Hsap\APP^{Aβ42.UAS.cUa}

(Panchal and Tiwari, 2020)

is exacerbated by Hsap\APP^{Aβ42.UAS.cUa}

(Panchal and Tiwari, 2020)

is exacerbated by Miro^GL01583

(Panchal and Tiwari, 2020)

is ameliorated by Miro^UAS.cGa

(Panchal and Tiwari, 2020)

is ameliorated by Ube4A^UAS.cSa

(Subramanian et al., 2021)

is ameliorated by Hsap\UBE4B^UAS.cSa

(Subramanian et al., 2021)

is exacerbated by Ube4A^KK101530

(Subramanian et al., 2021)

is exacerbated by mir-9b^{UAS.cluster.cSa.DsRed2}

(Subramanian et al., 2021)

is exacerbated by mir-9a^{UAS.cSa.DsRed2}

(Subramanian et al., 2021)

is exacerbated by mir-9c^UAS.cSa

(Subramanian et al., 2021)

is ameliorated by mir-9a^{sponge.UAS.mCherry}

(Subramanian et al., 2021)

is exacerbated by NUCB1^KK107501

(Yang et al., 2024)

is ameliorated by NUCB1^{Nesfatin1.UAS}

(Yang et al., 2024)

ameliorates Parkinson's disease

modeled by Hsap\SNCA^UAS.cTa

(Roy and Jackson, 2014)

model of Parkinson's disease

is ameliorated by Hsap\SNCA^UAS.cTa

(Roy and Jackson, 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Roy and Jackson, 2014)

abnormal locomotor behavior | adult stage | progressive

(Frenkel-Pinter et al., 2016)

abnormal neuroanatomy, with Scer\GAL4^RapGAP1-OK6

(Roy and Jackson, 2014)

abnormal neuroanatomy, with Scer\GAL4^VGlut1-OK371

(Roy and Jackson, 2014)

abnormal planar polarity, with Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

abnormal size, with Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

decreased rate of larval locomotory behavior, with Scer\GAL4^elav.PU

(Yang et al., 2024)

decreased size | adult stage, with Scer\GAL4^GMR.PFa

(Lee et al., 2020)

decreased size | adult stage, with Scer\GAL4^GMR.PU

(Yang et al., 2024)

increased cell death, with Scer\GAL4^GMR.PS

(Roy and Jackson, 2014)

short lived, with Scer\GAL4^elav.PU

(Yang et al., 2024)

short lived, with Scer\GAL4^nSyb.PS

(Fernius et al., 2017)

visible, with Scer\GAL4^GMR.PFa

(Kim et al., 2016)

visible | adult stage, with Scer\GAL4^Eq

(Yang et al., 2024)

visible | adult stage, with Scer\GAL4^GMR.PS

(Yeewa et al., 2024)

visible | adult stage, with Scer\GAL4^GMR.PU

(Yang et al., 2024, Frenkel-Pinter et al., 2016)

Phenotype Manifest In

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^RapGAP1-OK6

(Roy and Jackson, 2014)

eye, with Scer\GAL4^GMR.PFa

(Lee et al., 2020, Kim et al., 2016)

eye, with Scer\GAL4^GMR.PS

(Yeewa et al., 2024, Roy and Jackson, 2014, Chatterjee et al., 2009)

eye, with Scer\GAL4^GMR.PU

(Yang et al., 2024)

eye | adult stage, with Scer\GAL4^GMR.PU

(Frenkel-Pinter et al., 2016)

interommatidial bristle, with Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

larval segmental nerve | larval stage, with Scer\GAL4^VGlut1-OK371

(Roy and Jackson, 2014)

NMJ bouton | decreased number | larval stage, with Scer\GAL4^elav.PU

(Yang et al., 2024)

NMJ bouton | larval stage, with Scer\GAL4^RapGAP1-OK6

(Roy and Jackson, 2014)

ommatidium, with Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

rhabdomere, with Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

scutum, with Scer\GAL4^Eq

(Yang et al., 2024)

Detailed Description

Statement

Reference

Expressing Hsap\MAPT^UAS.wt under the control of Scer\GAL4^GMR.PFa induces eye degeneration (i.e. a small eye).

(Lee et al., 2020)

Expression of Hsap\MAPT^Scer\UAS.wt under the control of Scer\GAL4^nSyb.PS leads to significantly reduced adult lifespan.

(Fernius et al., 2017)

Expression of Hsap\MAPT^Scer\UAS.wt results in adult eye degeneration phenotype when driven by Scer\GAL4^GMR.PU and progressive loss of climbing ability in adult flies when driven by Scer\GAL4^elav-C155.

(Frenkel-Pinter et al., 2016)

Over-expression of Hsap\MAPT^Scer\UAS.wt driven in the eye by Scer\GAL4^GMR.PFa results in a small rough eye phenotype, compared to controls.

(Kim et al., 2016)

Flies expressing Hsap\MAPT^Scer\UAS.wt under the control of Scer\GAL4^GMR.PS show appreciable cell death (cleaved caspase activity) posterior to the morphogenetic furrow compared to controls.

Flies expressing Hsap\MAPT^Scer\UAS.wt in post-mitotic neurons under the control of Scer\GAL4^elav-C155 have severe motor deficits, with an ~67% decrease in climbing index.

The proportion of abnormal boutons in the neuromuscular junctions is increased compared to controls in larvae expressing Hsap\MAPT^Scer\UAS.wt under the control of Scer\GAL4^RapGAP1-OK6.

The segmental nerves of larvae expressing Hsap\MAPT^Scer\UAS.wt under the control of Scer\GAL4^VGlut-OK371 contain an increased number of axonal aggregates compared with controls.

(Roy and Jackson, 2014)

Adult eyes expressing Hsap\MAPT^Scer\UAS.wt under the control of Scer\GAL4^GMR.PS display a reduced, rough-eye phenotype, with fused and disordered ommatidia with missing and irregular bristles. Retinal sections show marked ommatidial disorganization and cell loss.

(Chatterjee et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by NUCB1^KK107501, Scer\GAL4^GMR.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PU has decreased size | adult stage phenotype, enhanceable by NUCB1^KK107501, Scer\GAL4^GMR.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^Eq has visible | adult stage phenotype, enhanceable by NUCB1^KK107501, Scer\GAL4^Eq

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^Eq has visible | adult stage phenotype, enhanceable by NUCB1^{Nesfatin1.UAS}, Scer\GAL4^Eq

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^elav.PU has decreased rate of larval locomotory behavior phenotype, enhanceable by NUCB1^KK107501, Scer\GAL4^elav.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa has decreased size | adult stage phenotype, enhanceable by mnb^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa

(Lee et al., 2020)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa has visible phenotype, enhanceable by mnb^UAS.cHa, Scer\GAL4^GMR.PFa

(Kim et al., 2016)

Hsap\MAPT^UAS.wt, Scer\GAL4^RapGAP1-OK6 has abnormal neuroanatomy phenotype, enhanceable by Hsap\SNCA^UAS.cTa, Scer\GAL4^RapGAP1-OK6

(Roy and Jackson, 2014)

Hsap\MAPT^UAS.wt, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy phenotype, enhanceable by Hsap\SNCA^UAS.cTa, Scer\GAL4^VGlut1-OK371

(Roy and Jackson, 2014)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has increased cell death phenotype, enhanceable by Hsap\SNCA^UAS.cTa, Scer\GAL4^GMR.PS

(Roy and Jackson, 2014)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has abnormal size phenotype, enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has abnormal planar polarity phenotype, enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has abnormal size phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has abnormal planar polarity phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

NOT Enhanced by

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has visible | adult stage phenotype, non-enhanceable by Ero1L^HMC05709, Scer\GAL4^GMR.PS

(Yeewa et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa has decreased size | adult stage phenotype, non-enhanceable by mnb^{D401ter.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Lee et al., 2020)

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by NUCB1^{Nesfatin1.UAS}, Scer\GAL4^GMR.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PU has decreased size | adult stage phenotype, suppressible | partially by NUCB1^{Nesfatin1.UAS}, Scer\GAL4^GMR.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^elav.PU has decreased rate of larval locomotory behavior phenotype, suppressible by NUCB1^{Nesfatin1.UAS}, Scer\GAL4^elav.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^elav.PU has short lived phenotype, suppressible by NUCB1^{Nesfatin1.UAS}, Scer\GAL4^elav.PU

(Yang et al., 2024)

NOT suppressed by

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has visible | adult stage phenotype, non-suppressible by Ero1L^HMC05709, Scer\GAL4^GMR.PS

(Yeewa et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa has decreased size | adult stage phenotype, non-suppressible by mnb^{D401ter.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Lee et al., 2020)

Enhancer of

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^ple.PF is an enhancer of abnormal neuroanatomy phenotype of Hsap\SNCA^UAS.cTa, Scer\GAL4^ple.PF

(Roy and Jackson, 2014)

Suppressor of

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^Eq is a suppressor | partially of increased mortality during development phenotype of NUCB1^{Nesfatin1.UAS}, Scer\GAL4^Eq

(Yang et al., 2024)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by NUCB1^KK107501, Scer\GAL4^GMR.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^Eq has scutum phenotype, enhanceable by NUCB1^KK107501, Scer\GAL4^Eq

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^Eq has scutum phenotype, enhanceable by NUCB1^{Nesfatin1.UAS}, Scer\GAL4^Eq

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^elav.PU has NMJ bouton | larval stage | decreased number phenotype, enhanceable by NUCB1^KK107501, Scer\GAL4^elav.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa has eye phenotype, enhanceable by mnb^UAS.Tag:FLAG, Scer\GAL4^GMR.PFa

(Lee et al., 2020)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa has eye phenotype, enhanceable by mnb^UAS.cHa, Scer\GAL4^GMR.PFa

(Kim et al., 2016)

Hsap\MAPT^UAS.wt, Scer\GAL4^RapGAP1-OK6 has embryonic/larval neuromuscular junction | larval stage phenotype, enhanceable by Hsap\SNCA^UAS.cTa, Scer\GAL4^RapGAP1-OK6

(Roy and Jackson, 2014)

Hsap\MAPT^UAS.wt, Scer\GAL4^RapGAP1-OK6 has NMJ bouton | larval stage phenotype, enhanceable by Hsap\SNCA^UAS.cTa, Scer\GAL4^RapGAP1-OK6

(Roy and Jackson, 2014)

Hsap\MAPT^UAS.wt, Scer\GAL4^VGlut1-OK371 has larval segmental nerve | larval stage phenotype, enhanceable by Hsap\SNCA^UAS.cTa, Scer\GAL4^VGlut1-OK371

(Roy and Jackson, 2014)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has ommatidium phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has eye phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has rhabdomere phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has ommatidium phenotype, enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has eye phenotype, enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has rhabdomere phenotype, enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

NOT Enhanced by

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has eye phenotype, non-enhanceable by Ero1L^HMC05709, Scer\GAL4^GMR.PS

(Yeewa et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa has eye phenotype, non-enhanceable by mnb^{D401ter.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Lee et al., 2020)

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by NUCB1^{Nesfatin1.UAS}, Scer\GAL4^GMR.PU

(Yang et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^elav.PU has NMJ bouton | larval stage | decreased number phenotype, suppressible by NUCB1^{Nesfatin1.UAS}, Scer\GAL4^elav.PU

(Yang et al., 2024)

NOT suppressed by

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by Ero1L^HMC05709, Scer\GAL4^GMR.PS

(Yeewa et al., 2024)

Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa has eye phenotype, non-suppressible by mnb^{D401ter.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Lee et al., 2020)

Enhancer of

Statement

Reference

Hsap\MAPT^UAS.wt, Scer\GAL4^ple.PF is an enhancer of dopaminergic neuron phenotype of Hsap\SNCA^UAS.cTa, Scer\GAL4^ple.PF

(Roy and Jackson, 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of mnb^Scer\UAS.cHa enhances the severity of the small rough eye phenotype seen in flies with expression of Hsap\MAPT^Scer\UAS.wt driven by Scer\GAL4^GMR.PFa.

(Kim et al., 2016)

Expression of Hsap\SNCA^Scer\UAS.cTa enhances the appreciable increase in cell death (cleaved caspase activity) posterior to the morphogenetic furrow seen in flies expressing Hsap\MAPT^Scer\UAS.wt under the control of Scer\GAL4^GMR.PS.

Expression of Hsap\SNCA^Scer\UAS.cTa enhances the severe motor deficits seen in flies expressing Hsap\MAPT^Scer\UAS.wt in post-mitotic neurons under the control of Scer\GAL4^elav-C155.

Expression of Hsap\SNCA^Scer\UAS.cTa enhances the proportion of abnormal boutons in the neuromuscular junctions of larvae expressing Hsap\MAPT^Scer\UAS.wt under the control of Scer\GAL4^RapGAP1-OK6.

Expression of Hsap\SNCA^Scer\UAS.cTa enhances the increase in segmental nerve axonal aggregates seen in larvae expressing Hsap\MAPT^Scer\UAS.wt under the control of Scer\GAL4^VGlut-OK371.

Expression of Hsap\MAPT^Scer\UAS.wt enhances the dopaminergic cell loss seen in the dorsolateral cluster of flies expressing Hsap\SNCA^Scer\UAS.cTa under the control of Scer\GAL4^ple.PF results in dopaminergic cell loss in the dorsolateral cluster.

(Roy and Jackson, 2014)

The rough-eye phenotype produced by Scer\GAL4^GMR.PS-mediated expression of Hsap\MAPT^Scer\UAS.wt is stronger when par-1^Scer\UAS.cSa is co-expressed; the mild disorganization of the retina observed in Scer\GAL4^GMR.PS, Hsap\MAPT^Scer\UAS.wt eyes is dramatically increased, resulting in smaller eyes with more missing bristles and fused ommatidia. Co-expression of par-1^Scer\UAS.cSa also increases the severity of the ommatidial polarity and rhabdomere loss phenotypes seen in Scer\GAL4^GMR.PS, Hsap\MAPT^Scer\UAS.wt retinas. These effects may be additive rather than synergistic.

Co-expression of sgg^Scer\UAS.cSa markedly enhances the Hsap\MAPT^Scer\UAS.wt, Scer\GAL4^GMR.PS eye phenotype, results in smaller eye with abnormally fused ommatidia and complete loss of bristles. The internal retinal architecture of the double transgenics is severely disrupted.

(Chatterjee et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

51363

w¹¹¹⁸; P{UAS-Tau.wt}7B

51362

w¹¹¹⁸; P{UAS-Tau.wt}1.13