FlyBase Allele Report: Dmel\MTF-1[αTub84B.PS]

General Information

Symbol

Dmel\MTF-1^αTub84B.PS

Species

D. melanogaster

Name

FlyBase ID

FBal0282052

Feature type

allele

Associated gene

Dmel\MTF-1

Associated Insertion(s)

Carried in Construct

P{tub-MTF-1.S}

Key Links

Transgenic product class

wild_type

(Saini et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Saini et al., 2011)

Mutagen

in vitro construct

(Saini et al., 2011)

Progenitor genotype

Carried in construct

P{tub-MTF-1.S}

Cytology

Description

αTub84B promoter sequences drive expression of full-length MTF-1 coding sequences.

(Saini et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

αTub84B

Encoded product / tool

MTF-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by park²⁵

(Saini et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of MTF-1^αTub84B.PS does not increase normal life span. Elevated MTF-1 expression not only prolongs the life span of park²⁵ mutant flies but also enhances survival during development.

(Saini et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

MTF-1^αTub84B.PS is a suppressor of lethal | pupal stage phenotype of MTF-1^140-1R, park²⁵

(Saini et al., 2011)

MTF-1^αTub84B.PS is a suppressor of short lived phenotype of park²⁵

(Saini et al., 2011)

MTF-1^αTub84B.PS is a suppressor of female sterile phenotype of park²⁵

(Saini et al., 2011)

MTF-1^αTub84B.PS is a suppressor of abnormal locomotor rhythm phenotype of park²⁵

(Saini et al., 2011)

MTF-1^αTub84B.PS is a suppressor of decreased fecundity | female phenotype of park²⁵

(Saini et al., 2011)

NOT Suppressor of

Statement

Reference

MTF-1^αTub84B.PS is a non-suppressor of male sterile phenotype of park²⁵

(Saini et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

MTF-1^αTub84B.PS is a suppressor of ovary phenotype of park²⁵

(Saini et al., 2011)

MTF-1^αTub84B.PS is a suppressor of oocyte phenotype of park²⁵

(Saini et al., 2011)

NOT Suppressor of

Statement

Reference

MTF-1^αTub84B.PS is a non-suppressor of individualization stage spermatid phenotype of park²⁵

(Saini et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of MTF-1^αTub84B.PS rescues the synthetic lethality found in park²⁵/MTF-1^140-1R double mutants.

Elevated expression of MTF-1, through expression of MTF-1^αTub84B.PS prolongs the life span of park²⁵ mutants significantly, from a median of 7 days for the mutants alone to 21 days. The maximal life span was extended from 12 to 41 days by the MTF-1^αTub84B.PS transgene. Elevated MTF-1 expression not only prolongs the life span of park²⁵ mutant flies but also enhances survival during development.

MTF-1^αTub84B.PS expression restores the basal levels of zinc, copper and iron found in park²⁵ homozygotes.

Strong MTF-1^αTub84B.PS expression dramatically improves the climbing ability of park²⁵ mutant flies. Furthermore, these flies generally move around very fast, and respond by running away when physically perturbed, jumping and occasionally displaying short flight episodes.

Female fecundity is completely rescued by MTF-1. When crossed with wild-type males, park²⁵ mutant females with MTF-1^αTub84B.PS reproduce the same number of progeny as a cross of wild-type males and females. Dissected ovaries from these females show a normalised structure with follicles formed in the germarium and mature stages in the posterior regions of the ovariole, with several oocytes ready for fertilisation. In contrast, the sterility phenotype of park²⁵ mutant males, which is due to defective spermatogenesis at the individualization step, is not rescued, indicated a role for park in male fertility.

(Saini et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

MTF-1^αTub84B.PS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close