FlyBase Allele Report: Scer\GAL4[ple.TH-D']

General Information

Symbol

Scer\GAL4^ple.TH-D'

Species

S. cerevisiae

Name

FlyBase ID

FBal0282526

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{ple-GAL4.TH-D'}

Also Known As

TH-D'-Gal4, THD'GAL4, D'-Gal4, TH-D', ΔTH-D'-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Liu et al., 2012)

Progenitor genotype

Scer\GAL4^ple.PF

(Liu et al., 2012)

Carried in construct

P{ple-GAL4.TH-D'}

Cytology

Description

Deletion of part of the ple sequence.

(Liu et al., 2012)

Allele components

Component

Use(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval dopaminergic DL2 neuron | subset

(Bjordal et al., 2014)

larval dopaminergic DL1 neuron | anterior | subset

(Bjordal et al., 2014)

adult stage

dopaminergic PAL neuron | subset

(Liu et al., 2012)

dopaminergic PPM2 neuron | subset

(Liu et al., 2012)

dopaminergic PPM3 neuron

(Liu et al., 2012)

dopaminergic PPL1 neuron | subset

(Pathak et al., 2015, Liu et al., 2012)

dopaminergic PPM3 neuron | subset

(Pathak et al., 2015)

dopaminergic neuron | subset

(Pathak et al., 2015)

Additional Information

Statement

Reference

ScerGAL4^ple.TH-D' drives expression in a subset of ple-positive dopaminergic neurons in the adult brain as well as in the thorax and abdomen.

(Pathak et al., 2015)

ScerGAL4^ple.TH-D' drives expression in two cells of the larval dopaminergic DL2 cluster, and three anterior cells of the larval dopaminergic DL1 cluster.

(Bjordal et al., 2014)

ScerGAL4^ple.TH-D' drives expression in a subset of the dopaminergic neurons in the adult brain labelled by ScerGAL4^ple.PF : around 1 PAL neuron, 1 PPM1 neuron, 2 PPM2 neurons, 6 PPM3 neurons and 10 PPL1 neurons. There was also some non-systematic expression in less than 1 neuron in the PAM cluster.

(Liu et al., 2012)

Marker for

Reflects expression of

ple

(Bjordal et al., 2014, Liu et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | larval stage, with slif^UY681

(Bjordal et al., 2014)

abnormal feeding behavior | adult stage, with Hsap\LRRK2^{G2019S.K1906M.UAS.Tag:FLAG}

(Cording et al., 2017)

abnormal feeding behavior | adult stage, with Hsap\LRRK2^{G2019S.UAS.cLa.Tag:FLAG}

(Cording et al., 2017)

abnormal feeding behavior | adult stage, with Hsap\LRRK2^I2020T.UAS

(Cording et al., 2017)

abnormal feeding behavior | larval stage, with slif^UY681

(Bjordal et al., 2014)

abnormal feeding behavior | larval stage | nutrition conditional, with Gcn2^RNAi.UAS.cUa

(Bjordal et al., 2014)

abnormal locomotor behavior | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Sun et al., 2018)

abnormal memory | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Galili et al., 2014)

abnormal memory | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Boto et al., 2019)

abnormal sleep | heat sensitive, with TrpA1^UAS.(B).cKa

(Liu et al., 2012)

flightless, with Orai^E180A.UAS

(Pathak et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^ple.TH-D' cells in adults in combination with a paired odor, results in a significant increase in short-term associative aversive olfactory memory, compared to controls.

(Boto et al., 2019)

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^ple.TH-D' cells in adults does not affect startle-induced climbing behavior, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^ple.TH-D' cells in adults significantly reduces startle-induced climbing behavior, compared to controls.

(Sun et al., 2018)

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^ple.TH-D' cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, significantly reduces aversive shock or temperature olfactory memory, compared to controls, with no effect on temperature avoidance.

(Galili et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93704

w^*; P{ple-GAL4.TH-D'}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^ple.TH-D'

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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