FlyBase Allele Report: Scer\GAL4[VP16.osk]

FB2025_02 , released April 17, 2025

FB2025_02 , released April 17, 2025

Allele: Scer\GAL4^VP16.osk

General Information

Symbol

Scer\GAL4^VP16.osk

Species

S. cerevisiae

Name

FlyBase ID

FBal0282702

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{osk-GAL4::VP16}

Also Known As

osk-GAL4, oskGal4, Osk>Gal4::VP16, oskar-Gal4, oskar GAL4, oskarGal4, osk Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Telley et al., 2012)

Progenitor genotype

Carried in construct

P{osk-GAL4::VP16}

Cytology

Description

A 1786bp osk fragment, which includes 1743bp upstream of the transcription start, the 15bp 5' UTR and 24bp of osk coding sequence is fused upstream of sequence encoding a GAL4::VP16 driver. The 3' UTR of αTub84B and Hsp70 is also present downstream of the coding sequence.

(Telley et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

(Wang et al., 2019)

female germline cell

(Jouette et al., 2019, Moschall et al., 2019)

adult stage | female oogenesis stage S9 -- S10

female germline cell

(Spracklen et al., 2014)

adult stage | female oogenesis stage S10B -- S13

(Groen et al., 2015)

female germline cell

(Leibfried et al., 2013)

Additional Information

Statement

Reference

Drives expression in female germline stem cells at mid-oogenesis.

(FlyBase Curators, 2017)

ScerGAL4^VP16.osk drives strong expression in female germline stem cells at mid-oogenesis. Authors note they specifically used the third chromosome insertion, P{osk-GAL4::VP16}F.

(Spracklen et al., 2014)

ScerGAL4^VP16.osk drives expression in the germline.

(Leibfried et al., 2013)

Marker for

Reflects expression of

(Jouette et al., 2019, Moschall et al., 2019, Bloomington Drosophila Stock Center, 2017, Groen et al., 2015, Spracklen et al., 2014, Leibfried et al., 2013)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell, with Ar1^HMS05657

(Mellentine et al., 2023)

border follicle cell, with cPges^GL01292

(Mellentine et al., 2023)

border follicle cell, with cPges^HMJ24151

(Mellentine et al., 2023)

egg chamber | oogenesis stage S9, with Avic\GFP^{EGFP.UASp.Lifeact}

(Spracklen et al., 2014)

egg chamber | oogenesis stage S9, with Disc\RFP^{tdT.UASp.F-Tractin}

(Spracklen et al., 2014)

egg chamber | oogenesis stage S10, with Hsap\UTRN^{ABD.UASp.EGFP}

(Spracklen et al., 2014)

egg chamber | oogenesis stage S10B, with Avic\GFP^{EGFP.UASp.Lifeact}

(Spracklen et al., 2014)

embryo | conditional, with RhoGEF2^{DHPH.UASp.CRY2(PHR),mCherry}

(Deneke et al., 2019)

filamentous actin, with Disc\RFP^{tdT.UASp.F-Tractin}

(Spracklen et al., 2014)

filamentous actin | oogenesis stage S9, with Avic\GFP^{EGFP.UASp.Lifeact}

(Spracklen et al., 2014)

filamentous actin | oogenesis stage S10, with Hsap\UTRN^{ABD.UASp.EGFP}

(Spracklen et al., 2014)

filamentous actin | oogenesis stage S10B, with Avic\GFP^{EGFP.UASp.Lifeact}

(Spracklen et al., 2014)

nucleolus | oogenesis, with Act5C^{UAS.Tag:FLAG,Tag:NLS(Unk)}

(Talbot et al., 2023)

nurse cell, with Act5C^{UAS.Tag:FLAG,Tag:NLS(Unk)}

(Talbot et al., 2023)

nurse cell, with Avic\GFP^{EGFP.UASp.Lifeact}

(Spracklen et al., 2014)

nurse cell, with Cdc42^RNAi.UASp

(Leibfried et al., 2013)

oocyte, with Avic\GFP^{EGFP.UASp.Lifeact}

(Spracklen et al., 2014)

ring canal, with Avic\GFP^{EGFP.UASp.Lifeact}

(Spracklen et al., 2014)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Atha\CIB1^{CIBN(ΔNLS).UASp.EGFP.Tag:PM(hKRAS)}, RhoGEF2^{DHPH.UASp.CRY2(PHR),mCherry}, Scer\GAL4^VP16.osk has actomyosin contractile ring | embryonic stage 5 phenotype, non-enhanceable by Df(3R)Exel6218/Df(3R)Exel6218

(Krueger et al., 2019)

Atha\CIB1^{CIBN(ΔNLS).UASp.EGFP.Tag:PM(hKRAS)}, RhoGEF2^{DHPH.UASp.CRY2(PHR),mCherry}, Scer\GAL4^VP16.osk has embryonic epidermis | embryonic stage 5 phenotype, non-enhanceable by Df(3R)Exel6218/Df(3R)Exel6218

(Krueger et al., 2019)

NOT suppressed by

Statement

Reference

Atha\CIB1^{CIBN(ΔNLS).UASp.EGFP.Tag:PM(hKRAS)}, RhoGEF2^{DHPH.UASp.CRY2(PHR),mCherry}, Scer\GAL4^VP16.osk has actomyosin contractile ring | embryonic stage 5 phenotype, non-suppressible by Df(3R)Exel6218/Df(3R)Exel6218

(Krueger et al., 2019)

Atha\CIB1^{CIBN(ΔNLS).UASp.EGFP.Tag:PM(hKRAS)}, RhoGEF2^{DHPH.UASp.CRY2(PHR),mCherry}, Scer\GAL4^VP16.osk has embryonic epidermis | embryonic stage 5 phenotype, non-suppressible by Df(3R)Exel6218/Df(3R)Exel6218

(Krueger et al., 2019)

Suppressor of

Statement

Reference

Scer\GAL4^VP16.osk/Khc^{401.UASp.Tag:MCP} is a suppressor of oocyte phenotype of Tm1^eg9/Tm1^eg1

(Gáspár et al., 2017)

sn^UASp.EGFP/Scer\GAL4^VP16.osk is a suppressor of nurse cell | oogenesis phenotype of Pxt^f01000

(Groen et al., 2015)

sn^UASp.EGFP/Scer\GAL4^VP16.osk is a suppressor of actin filament bundle | oogenesis phenotype of Pxt^f01000

(Groen et al., 2015)

sn^UASp.EGFP/Scer\GAL4^VP16.osk is a suppressor of nucleolus | oogenesis phenotype of Pxt^f01000

(Groen et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^VP16.osk/Tm1^{UASp.I.Emerald} rescues Tm1^eg9/Tm1^eg1

(Gáspár et al., 2017)

sn^UASp.EGFP/Scer\GAL4^VP16.osk rescues sn²⁸

(Groen et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

44241

w¹¹¹⁸; P{osk-GAL4::VP16}A11/CyO

44242

w¹¹¹⁸; P{osk-GAL4::VP16}F/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^VP16.osk

Scer\GAL4^{osk.T:Hsim\VP16}

Name Synonyms

Secondary FlyBase IDs

References (29)

Report Sections