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General Information
Symbol
Dmel\Pak3del
Species
D. melanogaster
Name
FlyBase ID
FBal0283074
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Recombination between the two progenitor insertions has deleted the genomic sequence between them, removing the entire Pak3 coding sequence.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Recombination between the two progenitor insertions has deleted the genomic sequence between them, removing the entire Pak3 coding sequence.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous embryos show a myoblast fusion defect. The average number of eve-positive nuclei per DA1 muscle is only 5.6 +/- 1.2, compared to the wild-type number of 10.7 +/- 1.6. Muscle cell fate specification, fusion competent myoblast (FCMs) migration and muscle cell adhesion appear normal in the mutant embryos.

The myoblast fusion defect seen in homozygous embryos is only slightly exacerbated if the embryos are derived from homozygous female germline clones (and thus lack maternal function in addition to lacking zygotic function).

A large number of F-actin foci per hemisegment remain in late stage 14 zygotically mutant embryos, in contrast to wild type, where the number of foci have significantly decreased by stage 15. The F-actin foci are generated in the FCMs (as in wild type), but have a more dispersed morphology than normal. The F-actin rich area in the mutant FCM extends further back into the cytoplasm compared to wild type (where it is restricted to the protrusive tip) and is decorated with an increased number of ribosomes, indicating the presence of loosely packed actin filaments. Most of the F-actin foci in late stage 14 mutant embryos are not associated with any inward curvature of the apposing founder cell/myotube membrane, suggesting that they fail to invade these cells (as occurs in wild type). A GFP diffusion assay and EM analysis suggest that fusion pore formation is blocked in the mutant embryos.

Expression of Pak3K322A.Scer\UAS.C.T:SV5\V5 under the control of Scer\GAL4twi.PG in a Pak3del background results in an almost complete failure of myoblast fusion in embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Pak3del is an enhancer of embryonic myoblast phenotype of SCARΔ37

Additional Comments
Genetic Interactions
Statement
Reference

Pakunspecified Pak3del double zygotic embryos show a similar myoblast fusion defect to that of Pak3del single zygotic embryos. Embryos both maternally and zygotically mutant for both Pakunspecified and Pak3del show a complete lack of myoblast fusion. Embryos maternally mutant for Pakunspecified and both maternally and zygotically mutant for Pak3del show an intermediate myoblast fusion defect between that of the double maternal+zygotic mutants and the double zygotic mutants.

Embryos that are maternally mutant for Pak3del and both maternally and zygotically mutant for Pakunspecified show a minor myoblast fusion defect.

Expression of PakScer\UAS.T:SV5\V5 under the control of Scer\GAL4twi.PG slightly, but significantly, rescues the myoblast fusion defect seen in zygotically mutant Pak3del embryos.

Pak3del WASp3 double mutant embryos have a more severe myoblast fusion defect than either single mutant.

Pak3del SCARΔ37 double mutant embryos have a more severe myoblast fusion defect than either single mutant.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Pak3Scer\UAS.T:SV5\V5 under the control of either Scer\GAL4twi.PG or Scer\GAL4sns.PK rescues the myoblast fusion defect seen in zygotically mutant Pak3del embryos.

Expression of Pak3Scer\UAS.T:SV5\V5 under the control of Scer\GAL4kirre-rP298 does not rescue the myoblast fusion defect seen in zygotically mutant Pak3del embryos.

Expression of Pak3H29L.H31L.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4twi.PG fails to rescue the myoblast fusion defect seen in zygotically mutant Pak3del embryos.

Expression of Pak3K322A.Scer\UAS.N.T:SV5\V5 under the control of Scer\GAL4twi.PG at 25[o]C fails to rescue the myoblast fusion defect seen in zygotically mutant Pak3del embryos. Expression of Pak3K322A.Scer\UAS.N.T:SV5\V5 under the control of Scer\GAL4twi.PG at 30[o]C, enhances the myoblast fusion defect seen in zygotically mutant Pak3del embryos.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Pak3del
Name Synonyms
Secondary FlyBase IDs
    References (1)