FB2025_01 , released February 20, 2025
Allele: Scer\GAL4Rab9.ATG
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General Information
Symbol
Scer\GAL4Rab9.ATG
Species
S. cerevisiae
Name
FlyBase ID
FBal0283199
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
PBac{I-SceI(FRT.Rab9-GAL4.ATG(loxP.3xP3-RFP))}
Also Known As
rab9-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Chan et al., 2011)
Progenitor genotype
Carried in construct
PBac{I-SceI(FRT.Rab9-GAL4.ATG(loxP.3xP3-RFP))}
Cytology
Description

Contains a genomic fragment centred on Rab9 in which part of the Rab9 open reading frame (extending from the ATG to the end of the ATG-containing exon) has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.

(Chan et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
Rab9
Encoded product / tool
GAL4
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      neuron | subset of larval brain
      (Chan et al., 2011)
      glial cell | subset of larval brain | intense
      (Chan et al., 2011)
      embryonic/larval salivary gland | restricted
      (Jin et al., 2012, Chan et al., 2011)
      neuron | subset of larval ventral nerve cord
      (Chan et al., 2011)
      eye disc | restricted
      (Jin et al., 2012, Chan et al., 2011)
      neuropil glial sheath | intense
      (Jin et al., 2012)
      Bolwig organ | restricted
      (Jin et al., 2012)
      embryonic/larval mushroom body | faint
      (Jin et al., 2012)
      embryonic/larval optic stalk surface glial cell
      (Jin et al., 2012)
      leg disc | restricted
      (Jin et al., 2012)
      pupal stage P6 -- P7
      neuron | subset of pupal brain

      Comment: 30-40h APF

      (Chan et al., 2011)
      glial cell | subset of pupal brain | intense

      Comment: 30-40h APF

      (Chan et al., 2011)
      neuropil glial sheath | intense

      Comment: 30-40h APF

      (Jin et al., 2012)
      neuron of adult optic lobe | faint

      Comment: 30-40h APF

      (Jin et al., 2012)
      eye photoreceptor cell | intense

      Comment: 30-40h APF

      (Jin et al., 2012)
      adult stage
      neuron | subset of adult brain
      (Chan et al., 2011)
      glial cell | subset of adult brain
      (Chan et al., 2011)
      adult ensheathing glial cell | intense
      (Jin et al., 2012)
      adult antennal lobe | faint
      (Jin et al., 2012)
      adult insulin secreting cell of pars intercerebralis | intense
      (Jin et al., 2012)
      subperineurial glial cell
      (Artiushin et al., 2018)
      Additional Information
      Statement
      Reference

      Scer\GAL4Rab9.ATG drives expression in the glial cells of the blood-brain barrier (BBB).

      (Artiushin et al., 2018)

      ScerGAL4Rab9.ATG drives expression in some neurons and glia in the larval brain, as well as in the developing photoreceptors in the eye disc. In the pupal and adult brain, expression in confined to glial cells and some neurons of the olfactory system.

      (Chan et al., 2011)
       
      Marker for
      Reflects expression of
      Rab9
      (Artiushin et al., 2018, Jin et al., 2012, Chan et al., 2011)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The PBac{I-SceI(FRT.Rab9-GAL4.ATG(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the Rab9 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous Rab9 locus.

      (Chan et al., 2011)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Rab9.ATG
      Name Synonyms
      Secondary FlyBase IDs
        References (5)