FlyBase Allele Report: Scer\GAL4[Crz.391]

General Information

Symbol

Scer\GAL4^Crz.391

Species

S. cerevisiae

Name

FlyBase ID

FBal0283229

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Crz-GAL4.391}

Also Known As

crz-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Tayler et al., 2012)

Progenitor genotype

Carried in construct

P{Crz-GAL4.391}

Cytology

Description

A 391bp fragment from upstream of the Crz putative transcriptional start site is fused upstream of sequence encoding a GAL4 driver.

(Tayler et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

Crz

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

larval corazonin neuron

(Hückesfeld et al., 2021)

adult stage | male

interneuron | subset of adult abdominal neuromere

(Tayler et al., 2012)

adult stage

adult optic lobe | restricted

(Tayler et al., 2012)

neuron of adult brain

(Tayler et al., 2012)

adult corazonin neuron | subset

(Davis et al., 2020)

Additional Information

Statement

Reference

Drives expression in corazonin neurons.

(FlyBase Curators, 2017)

ScerGAL4^Crz.391 drives expression in 2 pairs of interneurons in the abdominal ganglion of males and in non-dimorphic neurons in the optic lobe and central brain. The interneurons in the abdominal ganglion are also labelled with Crz and fru proteins. The central brain neurons do not co-express fru.

(Tayler et al., 2012)

Marker for

Reflects expression of

Crz

(Hückesfeld et al., 2021, Tayler et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Zer-Krispil et al., 2018)

abnormal mating | male limited | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Zer-Krispil et al., 2018)

abnormal memory | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Zer-Krispil et al., 2018)

abnormal phototaxis | adult stage | male limited | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Zer-Krispil et al., 2018)

increased body size | larval stage, with Crz^GD4673

(Imura et al., 2020)

increased body size | P-stage, with Crz^GD4673

(Imura et al., 2020)

increased body size | P-stage, with Crz^KK110968

(Imura et al., 2020)

increased body size | P-stage, with Oamb^JF01673

(Imura et al., 2020)

increased body size | P-stage, with Oamb^JF01732

(Imura et al., 2020)

male sterile, with Hsap\KCNJ2^UAS.EGFP

(Tayler et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 650 nm (red) light) of Scer\GAL4^Crz.391 cells induces ejaculation in males and results in a significant preference for an illuminated zone over non-illuminated areas, which is not seen in controls or in females; there is no effect on walking velocity, compared to controls; activation in combination with a paired odor induces a significant attractive olfactory memory that is not seen in controls; repeated activation over a few days results in a significant reduction in preference for ethanol-containing food, compared to controls.

(Zer-Krispil et al., 2018)

Activation of Scer\GAL4^Crz.391 neurons triggers ejaculation behavior; depending on the combination of UAS and light used, different numbers of flies show this behavior: around 20% with blue light activating Crei\ChR2^Scer\UAS.cSa; 0% with blue light activation of Crei\ChR2^{H134R.Scer\UAS.2A.T:Avic\GFP-EYFP} or Crei\ChR2^{C128T.Scer\UAS.T:Avic\GFP-YFP}; 20% with red (0% with green light) activating Crei\ChR1[C1V1.T-T.Scer\UAS. T:Avic \GFP-EYFP]; 100% with red or green light activating Crei\ChR1^{ReaChR.Scer\UAS.T:Crei\ChR2,T:Avic\GFP-mCitrine}.

(Inagaki et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

51976

w¹¹¹⁸; P{Crz-GAL4.391}3M

51977

w¹¹¹⁸; P{Crz-GAL4.391}4M

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^Crz.391

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

Open Close