FB2025_02 , released April 17, 2025
Allele: Dmel\NijAUAS.cBa
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General Information
Symbol
Dmel\NijAUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Broderick
FlyBase ID
FBal0283249
Feature type
allele
Associated gene
Dmel\NijA
Associated Insertion(s)
Carried in Construct
P{UAS-NijA.B}
Key Links
Transgenic product class
cDNA
(Broderick et al., 2012)
wild_type
(Broderick et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Broderick et al., 2012)
wild_type
(Broderick et al., 2012)
Mutagen
in vitro construct
(Broderick et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-NijA.B}
Cytology
Description

UASt regulatory sequences drive expression of the NijA RE5744 cDNA clone (this corresponds to the NijA-RA isoform).

(Broderick et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
NijA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of NijAScer\UAS.cBa under the control of either Scer\GAL4tub.PU or Scer\GAL4Act.PU results in embryos dying with morphological abnormalities around the time of cellularisation.

      Larvae expressing NijAScer\UAS.cBa under the control of Scer\GAL4Hml.Δ lack nearly all Hml-expressing hemocytes, both in circulation and in the lymph gland. The lymph glands stain with acridine orange, indicating cell death. TUNEL labelling indicates that the cell death is not due to apoptosis.

      (Broderick et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Phenotype Manifest In
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of BacA\p35Scer\UAS.cUa does not suppress the cell death seen in the lymph glands of larvae expressing NijAScer\UAS.cBa under the control of Scer\GAL4Hml.Δ.

      (Broderick et al., 2012)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      NijAScer\UAS.cBa
      NijAUAS.cBa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Broderick
      Secondary FlyBase IDs
        References (1)