FB2025_01 , released February 20, 2025
Allele: Dmel\HmgDUASp.cCa
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General Information
Symbol
Dmel\HmgDUASp.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Chen
FlyBase ID
FBal0283822
Feature type
allele
Associated gene
Dmel\HmgD
Associated Insertion(s)
Carried in Construct
P{UASp-HmgD.C}
Key Links
Transgenic product class
wild_type
(Chen et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Chen et al., 2008)
Mutagen
in vitro construct
(Chen et al., 2008)
Progenitor genotype
Carried in construct
P{UASp-HmgD.C}
Cytology
Description

UASp regulatory sequences drive expression of the HmgD coding region.

(Chen et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
HmgD
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing HmgDScer\UAS.P\T.cCa under the control of Scer\GAL4nos.PG show reduced viability compared to controls.

      Expression of HmgDScer\UAS.P\T.cCa under the control of Scer\GAL4Act.PU results in lethality. The larvae develop normally at the first and second instar larval stages. However the third instar larvae crawl to the vial walls and fail to pupariate properly. Most larvae die within a few days, but the few malformed pupae that do appear form five days after larval hatching. Some of these pupae are slightly tanned, but most body parts resemble larvae. Unlike in wild type, these abnormal bodies do not shorten at the beginning of pupariation. A few pupae are normally tanned, but the heads are still connected to the larval spicules and so do not evert at all, and the larval hooks are not ejected. Feeding ecdysone to the larvae does is unable to suppress this lethality.

      (Chen et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      HmgDScer\UAS.P\T.cCa
      HmgDUASp.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Chen
      Secondary FlyBase IDs
        References (1)