Blocking the output (using shi1.UAS at 33[o]C) of Scer\GAL4LpR1-0104-G4 neurons shortly before and during training significantly impairs long-term olfactory memory in starved flies trained with sucrose, compared to controls; when Scer\GAL80R48B04 is added to this combination to reduce the the affected neurons to a subset, long-term memory is still significantly impaired in starved flies trained with sucrose and short term-memory is also significantly impaired in starved flies trained with arabinose, compared to controls.
Acute thermogenetic activation (using TrpA1UAS.(B).cKa at 33[o]C) of Scer\GAL4LpR1-0104-G4 neurons results in a significant long-term preference for a paired odor (artificial memory) in starved flies, but not flies that are fed before or after training, compared to controls.
Blocking synaptic transmission (using shi1.Scer\UAS at restrictive temperatures) from Scer\GAL4LpR1-0104-G4 neurons during acquisition significantly impairs learning in a differential aversive conditioning paradigm (in the Y[] vs Z[] relative condition but not the X[] vs Y[] absolute condition). Flies undergo conditioning at a restrictive temperature (33[o]C), whereas testing is at a permissive temperature (23[o]C); there are no significant differences when training and testing is at permissive temperatures.