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General Information
Symbol
Scer\GAL4LpR1-0104-G4
Species
S. cerevisiae
Name
FlyBase ID
FBal0283997
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
0104-GAL4
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    PBac{IT.GAL4}LpR10104-G4
    Product class / Tool use(s)
    Expression Data
    Reporter Expression
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Information
    Statement
    Reference

    Scer\GAL4LpR1-0104-G4 labels ~55 mostly dopaminergic PAM neurons. A FLP/FRT intersectional strategy shows expression in 10-20 dopaminergic neurons, innervating mushroom body beta' lobe slice 2, mushroom body gamma lobe slice 4, and TH negative neuron associated with PAM clusters that connect mushroom body gamma lobe slices 1, 2 & 4 when combined with Ecol\lexAGMR48B04. This intersection does not label neurons innervating mushroom body gamma lobe slice 5.

    Using GFP reconstitution across synaptic partners (GRASP) in a subset of dopaminergic neurons labelled by Scer\GAL4LpR1-0104-G4 and octopaminergic neurons labelled by Scer\GAL4Tdc2.PC, reveals expression of GFP in the anterior medial protocerebrum.

     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Blocking the output (using shi1.UAS at 33[o]C) of Scer\GAL4LpR1-0104-G4 neurons shortly before and during training significantly impairs long-term olfactory memory in starved flies trained with sucrose, compared to controls; when Scer\GAL80R48B04 is added to this combination to reduce the the affected neurons to a subset, long-term memory is still significantly impaired in starved flies trained with sucrose and short term-memory is also significantly impaired in starved flies trained with arabinose, compared to controls.

    Acute thermogenetic activation (using TrpA1UAS.(B).cKa at 33[o]C) of Scer\GAL4LpR1-0104-G4 neurons results in a significant long-term preference for a paired odor (artificial memory) in starved flies, but not flies that are fed before or after training, compared to controls.

    Blocking synaptic transmission (using shi1.Scer\UAS at restrictive temperatures) from Scer\GAL4LpR1-0104-G4 neurons during acquisition significantly impairs learning in a differential aversive conditioning paradigm (in the Y[[60]] vs Z[[30]] relative condition but not the X[[0]] vs Y[[60]] absolute condition). Flies undergo conditioning at a restrictive temperature (33[o]C), whereas testing is at a permissive temperature (23[o]C); there are no significant differences when training and testing is at permissive temperatures.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    Scer\GAL40104
    Scer\GAL4LpR1-0104-G4
    Name Synonyms
    Secondary FlyBase IDs
      References (6)