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General Information
Symbol
Dmel\Hr51miRNA.UAS.a
Species
D. melanogaster
Name
FlyBase ID
FBal0284091
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-miR-unf
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS drives expression of miRNAs that target two sites in the Hr51 coding sequence, one at 289-310 nt and the other at 1130-1151 nt.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In adults previously entrained under 12h:12h light:dark cycles, the constitutive expression of Hr51miRNA.Scer\UAS.a driven by Scer\GAL4Pdf.PU, or adulthood-only expression driven by Scer\GAL41118 and temporally-controlled by Gal80[ts], leads to a severe decrease in the proportion of circadian rhythmic individuals, despite of insignificant changes in the period, upon shift to constant darkness conditions, as compared to controls.

Flies expressing Hr51miRNA.Scer\UAS.a ubiquitously under the control of Scer\GAL4αTub84B.PL display abnormal activity patterns in LD conditions (12 hours light: 12 hours dark), and a large majority (94.3%) are arrhythmic in DD conditions (constant darkness). When Hr51miRNA.Scer\UAS.a is expressed in the LNvs (ventral Lateral Neurons) under the control of either Scer\GAL4P2.4.Pdf or Scer\GAL41118 the flies display normal activity patterns in LD, but more than 60% of flies are arrhythmic and 10-20% show complex rhythms. A similar effect is seen when Hr51miRNA.Scer\UAS.a is expressed in all clock neurons under the control of Scer\GAL4tim.PU. Expression of Hr51miRNA.Scer\UAS.a in the majority of mushroom body neurons under the control of Scer\GAL4ey-OK107 results in only slight phase delays in LD, but otherwise has no major effects in circadian locomotor rhythms in either LD or DD.

Expression of Hr51miRNA.Scer\UAS.a in LNvs only during adulthood (by restricting expression of Scer\GAL4P2.4.Pdf during development using Scer\GAL80ts.αTub84B) results in normal rhythmic behavior in both LD and DD, but the average period of the free-running rhythms is slightly longer than in controls. Flies expressing Hr51miRNA.Scer\UAS.a only during development show normal behavior in LD. The majority of these flies are arrhythmic in DD.

Expression using Scer\GAL4ey-OK107 results in 100% of mushroom body lobes stopping shortly at the end of peduncle.

Scer\GAL4ey-OK107-mediated expression of Hr51miRNA.Scer\UAS.a results in loss of the five adult mushroom body-characteristic axon lobes in all cases. Instead, a ball-like structure is present around the end of the peduncle. The mushroom bodies were otherwise grossly normal. Scer\GAL4ey-OK107-mediated expression of Hr51miRNA.Scer\UAS.a in a Df(2R)ED2426/+ background does not affect the severity of this mushroom body axonal lobe defect.

Most mushroom bodies of Scer\GAL4ey-OK107 Hr51miRNA.Scer\UAS.a mid-third instar larvae are grossly normal, although 12% have thinner dorsal lobes.

Scer\GAL4ey-OK107 Hr51miRNA.Scer\UAS.a mushroom bodies display no obvious morphological defect at 6 hr APF. By contrast, at 18 hr APF when the larval gamma lobes are largely pruned, alpha'/beta' lobes do not exist. At 24 hr APF, no axon extends beyond the peduncle end, indicating defects in both gamma axon re-extension and alpha/beta bundle formation. By 48 hr APF, instead of seeing all five mushroom body lobes, a ball-like structure bulges around the terminus of the peduncle - axons elaborate locally but fail to project into discrete domains.

Expression of Hr51miRNA.Scer\UAS.a in mature larval gamma neurons using Scer\GAL4Tab2-201Y results in missing adult gamma lobes despite presence of other mushroom body lobes. Pruning of the larval-specific gamma lobes occurs normally.

Expression of Hr51miRNA.Scer\UAS.a in most, if not all, post-mitotic mushroom body neurons using Scer\GAL4Mef2.247 results in selective loss of the adult gamma lobe.

Expression of Hr51miRNA.Scer\UAS.a in newborn neurons using Scer\GAL4ase.neuro does not affect larval gamma axon projections but drastically arrests the nascent alpha'/beta' or alpha/beta axon bundles around the peduncle.

Scer\GAL4ey-OK107-mediated induction of Hr51miRNA.Scer\UAS.a from mid-3rd instar and prior to birth of most alpha'/beta' neurons (using the Scer\GAL80[ts] system) disrupts all mushroom body lobes. In contrast, inducing expression after adult eclosion leaves the gross mushroom body morphology intact. When induced around puparium formation after the production of alpha'/beta' neurons, the alpha'/beta' lobes are grossly normal but the Fas2-positive alpha/beta lobes are malformed.

The axonal processes of flip-out clones in Scer\GAL4ey-OK107 Hr51miRNA.Scer\UAS.a mushroom bodies wander around in the ball-like truncated lobes, often making unusual back turns or loops.

Single Scer\GAL4ey-OK107 Hr51miRNA.Scer\UAS.a gamma neurons (generated by MARCM) exhibit abnormal axon trajectories within grossly normal mushroom bodies. These misguided axons are not stalled at the peduncle end.

About 10% of the strongest Scer\GAL4ey-OK107 Hr51miRNA.Scer\UAS.a mushroom bodies show ectopic Fas2-positive axon bundles extending through the calyx into abnormal targets rather than migrating along the peduncle.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Adulthood co-expression of Hr51miRNA.Scer\UAS.a and RnbGD12299, driven by Scer\GAL41118 and temporally-controlled by Gal80[ts], enhances the increase in circadian period observed upon the adulthood expression of either Hr51miRNA.Scer\UAS.a alone or RnbGD12299 alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hr51miRNA.Scer\UAS.a
Hr51miRNA.UAS.a
Name Synonyms
Secondary FlyBase IDs
    References (4)