FlyBase Allele Report: Dmel\sicily[E]

General Information

Symbol

Dmel\sicily^E

Species

D. melanogaster

Name

FlyBase ID

FBal0284113

Feature type

allele

Associated gene

Dmel\sicily

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Yamamoto et al., 2013-, Zhang et al., 2013)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q133term.

(Zhang et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,892,829..11,892,829 [+]

Nucleotide change:

C11892829T

Amino acid change:

Q133term | sicily-PA; Q133term | sicily-PB

Reported amino acid change:

Q133term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zhang et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Leigh disease

CEA

(Zhang et al., 2013)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | progressive | somatic clone

(Liu et al., 2017)

abnormal neurophysiology | progressive | somatic clone

(Zhang et al., 2013)

lethal - all die during pupal stage

(Zhang et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Whole-eye sicily^E mutant clones in adult flies exhibit severe lipid droplet accumulations in glial cells and photoreceptor neurodegeneration.

(Liu et al., 2017)

sicily^E mutants exhibit abundant lipid droplet accumulations in pigment and epithelial glia. In addition to the retina, lipid droplets also accumulate in the epithelial glia of the lamina.

sicily^E mutant visual system clones (generated with hid^GMR.PG) exhibit lipid droplet accumulation in the glia.

The morphology of sicily^E mutant photoreceptors is mostly normal or only mildly affected at day 1. They begin to degenerate several days after eclosion, with elongated or diffuse rhabdomeres. One-day old sicily^E clones exhibit approximately 10 lipid droplets per ommatidium. Upon aging, these clones display a significant decrease in the number of lipid droplets.

(Liu et al., 2015)

Mutant flies containing homozygous eyes (generated using the eyFLP method) show degenerative electroretinogram (ERG) defects which worsen with age. The amplitude of the ERG is reduced in 1 day old flies and is almost completely abolished after 24 days. Ommatidia contain the normal number of rhabdomeres and have a normal trapezoidal organisation in newly eclosed flies containing homozygous eyes. However, the pigment cells are vastly expanded and have highly aberrant morphology. Some rhabdomeres are missing in 10 day old flies, and many photoreceptors appear rough and/or vacuolated. At 24 days, the rhabdomeres are barely recognisable or are missing. Mitochondria are enlarged in the retinas of newly eclosed flies, by day 10, the mutant mitochondria are swollen and vacuolated with dissociated cristae and by day 24 they are barely recognisable.

(Zhang et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Sln^GD1940/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Sln^KK104306/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Fatp1^k10307/Fatp[+]

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by GLaz^GD4806/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by GLaz^KK106377/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by NLaz[+]/NLaz^NW5

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by out^GD3448/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by out^KK104187/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Sln^D1/Sln[+]

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Bsg^SH1217/Bsg[+]

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Fatp1^KK104809/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Fatp1^HMC03111/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Fatp1^HMC03960/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Fatp1^HMC04206/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has abnormal neurophysiology | progressive phenotype, suppressible by Hsap\NDUFAF6^UAS.cZa/Scer\GAL4^da.G32

(Zhang et al., 2013)

sicily^E has lethal phenotype, suppressible by Hsap\NDUFAF6^UAS.cZa/Scer\GAL4^da.G32

(Zhang et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by Sln^GD1940/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible by Sln^GD1940/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Sln^GD1940/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by Sln^KK104306/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible by Sln^KK104306/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Sln^KK104306/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by out^GD3448/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible by out^GD3448/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by out^GD3448/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by out^KK104187/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible by out^KK104187/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by out^KK104187/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible | partially by Sln^D1/Sln[+]

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible | partially by Sln^D1/Sln[+]

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Sln^D1/Sln[+]

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible | partially by Bsg^SH1217/Bsg[+]

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible | partially by Bsg^SH1217/Bsg[+]

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Bsg^SH1217/Bsg[+]

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by Fatp1^KK104809/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible by Fatp1^KK104809/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Fatp1^KK104809/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by Fatp1^HMC03111/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible by Fatp1^HMC03111/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Fatp1^HMC03111/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by Fatp1^HMC03960/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible by Fatp1^HMC03960/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Fatp1^HMC03960/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by Fatp1^HMC04206/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has lipid droplet | somatic clone | adult stage phenotype, suppressible by Fatp1^HMC04206/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Fatp1^HMC04206/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by Fatp1^k10307/Fatp[+]

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by GLaz^GD4806/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has adult optic lobe glial cell | somatic clone phenotype, suppressible by GLaz^KK106377/Scer\GAL4^ey.PU

(Liu et al., 2017)

sicily^E has photoreceptor | somatic clone | adult stage | progressive phenotype, suppressible by NLaz[+]/NLaz^NW5

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of any of the following: Sln^GD1940, Sln^KK104306, out^GD3448, out^KK104187, Fatp^KK104809, Fatp^HMC03111, Fatp^HMC03960 Fatp^HMC04206, GLaz^GD4806 or GLaz^KK106377 in the ey>flip whole-eye clones mutant for sicily^E ameliorates the lipid droplet accumulation in retinal glial cells in adult flies and also improves the photoreceptor degeneration phenotype. Significant amelioration of both phenotypes is also observed upon combination with one copy of either Sln^D1 or Bsg^SH1217 and the neurodegeneration phenotype is improved also by Fatp^k10307 or NLaz^NW5 heterozygosity.

(Liu et al., 2017)

A bsk¹ heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in sicily^E mutants.

A SREBP¹⁸⁹ heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in sicily^E mutants.

Visual system knockdown of bsk, through expression of bsk^GD10555 under the control of Scer\GAL4^ey.PH in a sicily^Emutant background significantly reduces lipid droplet accumulation.

Expression of Lip4^Scer\UAS.cLa in sicily^E mutant clones partially restores the shape, density and number of identifiable rhabdomeres, indicating that reducing lipid droplet accumulation partially alleviates neurodegeneration.

Expression of bmm^Scer\UAS.cGa in sicily^E mutant clones partially restores the shape, density and number of identifiable rhabdomeres, indicating that reducing lipid droplet accumulation partially alleviates neurodegeneration.

(Liu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^da.G32 in sicily^E mutant larvae reduces the overall level of reactive oxygen species and considerably suppresses the lipid droplet accumulation seen in these flies.

(Liu et al., 2015)

Expression of Hsap\NDUFAF6^Scer\UAS.cZa under the control of Scer\GAL4^da.G32 rescues the lethality and progressive electroretinogram caused by sicily^E.

(Zhang et al., 2013)

Complementation and Rescue Data

Fails to complement

sicily^EY02706

(Zhang et al., 2013)

Rescued by

sicily^E is rescued by sicily^+tgRes

(Zhang et al., 2013)

sicily^E is rescued by Scer\GAL4^da.G32/sicily^UAS.cZa

(Zhang et al., 2013)

sicily^E is rescued by sicily^mCherry

(Zhang et al., 2013)

Partially rescued by

sicily^E is partially rescued by sicily^-MTS.UAS/Scer\GAL4^da.G32

(Zhang et al., 2013)

Comments

sicily^+tgRes rescues the progressive degenerative defects seen in flies containing homozygous sicily^E eyes (generated using the eyFLP method).

sicily^{T:Disc\RFP-mCherry} rescues the lethality and electroretinogram defects of sicily^E.

Expression of sicily^{-MTS.Scer\UAS} under the control of Scer\GAL4^da.G32 rescues the lethality of sicily^E animals. The rescued animals show subtle defects: a partial loss of the 'off' transient in the electroretinogram, enlarged mitochondria at day 1 and some loss of photoreceptors at day 10.

(Zhang et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

52394

y¹ w^* sicily^E P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

XE07E

(Zhang et al., 2013)

sicily^E

(Liu et al., 2017, Liu et al., 2015, Yamamoto et al., 2013-)

Name Synonyms

Secondary FlyBase IDs

References (4)

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