UAS regulatory sequences drive expression of the bigmax coding region, which is tagged at the C-terminal end with Tag:FLAG.
bigmaxUAS.Tag:FLAG/Scer\GAL4αTub84B.PL rescues bigmax1
bigmaxUAS.Tag:FLAG/Scer\GAL4ppl.PP rescues bigmax1
bigmaxUAS.Tag:FLAG/Scer\GAL4r4 rescues bigmax1
bigmaxUAS.Tag:FLAG/Scer\GAL4elav.PLu fails to rescue bigmax1
bigmaxUAS.Tag:FLAG/Scer\GAL4Mef2.PR fails to rescue bigmax1
Expression of bigmaxScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4αTub84B.PL, Scer\GAL4ppl.PP or Scer\GAL4r4 rescues the sugar intolerance and pupal lethality of bigmax1 mutants.
Expression of bigmaxScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4r4, but not Scer\GAL4Mef2.PR, is sufficient to normalise circulating glucose levels in bigmax1 larvae.
Expression of bigmaxScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4elav.PLu or Scer\GAL4Mef2.PR does not significantly improve the sugar tolerance or survival of bigmax1 mutants.