FB2025_01 , released February 20, 2025
Allele: Hsap\MAPTUAS.1N4R
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General Information
Symbol
Hsap\MAPTUAS.1N4R
Species
H. sapiens
Name
FlyBase ID
FBal0284417
Feature type
allele
Associated gene
Hsap\MAPT
Associated Insertion(s)
Carried in Construct
P{UAS-tau.1N4R}
Key Links
Transgenic product class
wild_type
(Folwell et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Folwell et al., 2010)
Mutagen
in vitro construct
(Folwell et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-tau.1N4R}
Cytology
Description

UAS regulatory sequences drive expression of a 1N4R Hsap\MAPT-isoform.

(Folwell et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\MAPT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  tauopathy
      CEA
      (Folwell et al., 2010)
      model of  Alzheimer's disease
      CEA with Hsap\APPAβ42.UAS.cUa
      (Folwell et al., 2010)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  tauopathy
      is ameliorated by dshUAS.Tag:MYC
      (Folwell et al., 2010)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Motor neurons of larvae expressing Hsap\MAPTScer\UAS.1N4R under the control of Scer\GAL4D42 display axonal transport defects.

      Expression of Hsap\MAPTScer\UAS.1N4R under the control of Scer\GAL4D42 leads to subtle morphological aberrations of larval neuromuscular junctions (NMJs) which includes the appearance of "satellite" boutons and thinning of inter-bouton regions.

      Adult flies expressing Hsap\MAPTScer\UAS.1N4R under the control of Scer\GAL4unspecified exhibit locomotor impairments and reduced lifespan that becomes more severe with age.

      For the first 5 days post-eclosion, the climbing ability of flies expressing Scer\GAL4unspecified>Hsap\MAPTScer\UAS.1N4R does not differ from that of control flies. However, at every time point after this period, significantly fewer Hsap\MAPTScer\UAS.1N4R-expressing flies show normal climbing ability when compared with age-matched controls.

      (Folwell et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      o-expression of Hsap\APPAβ42.Scer\UAS.cUa with Hsap\MAPTScer\UAS.1N4R under the control of Scer\GAL4Toll-6-D42 enhances the axonal transport phenotype resulting from the expression of Hsap\MAPTScer\UAS.1N4R alone.

      Co-expression of Hsap\APPAβ42.Scer\UAS.cUa with Hsap\MAPTScer\UAS.1N4R under the control of Scer\GAL4Toll-6-D42 exacerbates the neuromuscular junction (NMJ) aberrations seen in mutants expressing any one of the transgenes alone, with more evidence of both "satellite" boutons and thinning of the inter-bouton regions.

      Co-expression of Hsap\MAPTScer\UAS.1N4R and Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4unspecified enhances the locomotor impairment of flies expressing Scer\GAL4unspecified>Hsap\MAPTScer\UAS.1N4R alone. As the double transgenic flies age, their climbing abilities steadily deteriorate.

      Co-expression of Hsap\MAPTScer\UAS.1N4R and Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4unspecified enhances the longevity phenotypes that are seen in flies expressing any of the two transgenes alone. LiCl treatment ameliorates the survival defects.

      Co-expression of dshScer\UAS.T:Hsap\MYC with Hsap\MAPTScer\UAS.1N4R under the control of Scer\GAL4unspecified suppresses the axonal transport defects and locomotor impairments induced by the expression of Hsap\MAPTScer\UAS.1N4R alone.

      (Folwell et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\MAPTScer\UAS.1N4R
      Hsap\MAPTUAS.1N4R
      Name Synonyms
      Secondary FlyBase IDs
        References (1)