FlyBase Allele Report: Dmel\Nmdar1[N631Q.UAS]

General Information

Symbol

Dmel\Nmdar1^N631Q.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0284429

Feature type

allele

Associated gene

Dmel\Nmdar1

Associated Insertion(s)

Carried in Construct

P{UAS-Nmdar1.N631Q}

Key Links

Transgenic product class

missense_variant

(Miyashita et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Miyashita et al., 2012)

Mutagen

in vitro construct

(Miyashita et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Nmdar1.N631Q}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of Nmdar1, which contains the amino acid replacement N631Q.

(Miyashita et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nmdar1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^C42

(Miyashita et al., 2012)

abnormal memory, with Scer\GAL4^c309

(Miyashita et al., 2012)

abnormal memory, with Scer\GAL4^elav.PU

(Miyashita et al., 2012)

abnormal memory, with Scer\GAL4^ey-OK107

(Miyashita et al., 2012)

abnormal memory, with Scer\GAL4^Feb170

(Miyashita et al., 2012)

abnormal memory | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Miyashita et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing Nmdar1^{N631Q.Scer\UAS} under the control of Scer\GAL4^elav.PU show normal learning (memory measured immediately after conditioning) and short-term memory (assayed 1 hour after training) in an aversive olfactory conditioning assay. One-day memory after spaced training (long-term memory) is reduced compared to wild type, while one-day memory after massed training (anesthesia-resistant memory) is normal.

Acute expression of Nmdar1^{N631Q.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} (by feeding 1mM RU486 starting one day prior to training) disrupts one-day long term memory in an aversive olfactory conditioning assay, while anesthesia-resistant memory is unaffected.

Expression of Nmdar1^{N631Q.Scer\UAS} under the control of either Scer\GAL4^Feb170, Scer\GAL4^ey-OK107, Scer\GAL4^c309 or Scer\GAL4^C42 disrupts long-term memory in an aversive olfactory conditioning assay, while anesthesia-resistant memory is unaffected.

(Miyashita et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/Nmdar1^N631Q.UAS rescues Nmdar1^EP331

(Miyashita et al., 2012)

Comments

Expression of Nmdar1^{N631Q.Scer\UAS} under the control of Scer\GAL4^elav.PU rescues the learning defects seen in Nmdar1^EP331 flies.

(Miyashita et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nmdar1^{N631Q.Scer\UAS}

Nmdar1^N631Q.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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