FlyBase Allele Report: Dmel\Dmtn[UAS.cHa]

General Information

Symbol

Dmel\Dmtn^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hopkins

FlyBase ID

FBal0284517

Feature type

allele

Associated gene

Dmel\Dmtn

Associated Insertion(s)

Carried in Construct

P{UAS-Dmtn.H}

Key Links

Transgenic product class

cDNA

(Hopkins, 2013)

wild_type

(Hopkins, 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hopkins, 2013)

wild_type

(Hopkins, 2013)

Mutagen

in vitro construct

(Hopkins, 2013)

Progenitor genotype

Carried in construct

P{UAS-Dmtn.H}

Cytology

Description

UASt regulatory sequences drive expression of Dmtn cDNA (from clone RE27645).

(Hopkins, 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dmtn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Dmtn^e01970), with Scer\GAL4^repo

(Hopkins, 2013)

short lived (with Dmtn^e01970), with Scer\GAL4^repo

(Hopkins, 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

10 day old Dmtn^e01970 mutant flies in which Dmtn expression has been rescued in glia (through expression of Dmtn^Scer\UAS.cHa under the control of Scer\GAL4^repo) show evidence of neurodegeneration in the form of vacuoles. These vacuoles are rarely seen in 10 day old flies expressing Dmtn^Scer\UAS.cHa in neurons under the control of Scer\GAL4^elav-C155, or expressing Dmtn^Scer\UAS.cHa in both neurons and glia. Vacuoles are not seen in any of the rescued flies at one day old.

Dmtn^e01970 mutant flies expressing Dmtn^Scer\UAS.cHa in glia under the control of Scer\GAL4^repo have a severely reduced lifespan compared to those expressing Dmtn^Scer\UAS.cHa in neurons under the control of Scer\GAL4^elav-C155, or in both neurons or glia.

(Hopkins, 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Dmtn^UAS.cHa, Scer\GAL4^elav-C155 is a suppressor of partially lethal - majority die phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Hopkins, 2013)

Dmtn^UAS.cHa, Scer\GAL4^elav-C155 is a suppressor of visible phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Hopkins, 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dmtn^UAS.cHa, Scer\GAL4^elav-C155 is a suppressor of wing phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Hopkins, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Dmtn^Scer\UAS.cHa suppresses the predominantly male semi-lethality and wing expansion defects seen when Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} is expressed in neurons under the control of Scer\GAL4^elav-C155.

(Hopkins, 2013)

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/Dmtn^UAS.cHa rescues Dmtn^e01970

(Hopkins, 2013)

Scer\GAL4^elav-C155, Dmtn^UAS.cHa, Scer\GAL4^repo rescues Dmtn^e01970

(Hopkins, 2013)

Partially rescues

Dmtn^UAS.cHa/Scer\GAL4^repo partially rescues Dmtn^e01970

(Hopkins, 2013)

Comments

Expression of Dmtn^Scer\UAS.cHa under the control of either Scer\GAL4^elav-C155 (neurons), Scer\GAL4^repo (glia) or both, rescues the lethality associated with Dmtn^e01970. The optic lobes of all Dmtn^e01970 flies rescued by expression of Dmtn^Scer\UAS.cHa in either just neurons or both neurons and glia are normal in appearance. The optic lobes of flies rescued in the glia only are less developed, as shown by a wider angle between the lamina and medulla neuropils, due to partial rotation of the medulla neuropil and by aberrant targeting of retinal axons.

(Hopkins, 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dmtn^Scer\UAS.cHa

Dmtn^UAS.cHa

Tmcc^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hopkins

Secondary FlyBase IDs

References (1)

Report Sections

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