FlyBase Allele Report: Dmel\InR[UAS.cUa]

General Information

Symbol

Dmel\InR^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0284595

Feature type

allele

Associated gene

Dmel\InR

Associated Insertion(s)

Carried in Construct

P{UAS-InR.U}

Also Known As

UAS-InR, UAS-InR^wt, UAS INR, UAS-dInR

Key Links

Transgenic product class

wild_type

(Wu et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wu et al., 2011)

Mutagen

in vitro construct

(Wu et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-InR.U}

Cytology

Description

UAS regulatory sequences drive expression of InR.

(Wu et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

InR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cervical cancer

CEA with HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa

(Padash Barmchi et al., 2016)

model of high grade glioma

CEA

(Witte et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval optic stalk, with Scer\GAL4^repo

(Witte et al., 2009)

eye, with Scer\GAL4^GMR.PF

(Buszard et al., 2013, Wu et al., 2011)

gamma Kenyon cell, with Scer\GAL4^ey-OK107

(Lin et al., 2013)

larval glial cell, with Scer\GAL4^repo

(Witte et al., 2009)

midgut | adult stage, with Scer\GAL4^NP5130

(Wang et al., 2014)

mushroom body, with Scer\GAL4^ey-OK107

(Lin et al., 2013)

Detailed Description

Statement

Reference

Expression of InR^UAS.cUa under the control of the split-GAL4 pair of Scer\GAL4^DBD.AkhR and Hsap\RELA^AD.nSyb in adult females results in a delay in starvation-induced hyperactivity, compared to controls.

(Yu et al., 2016)

The temporally regulated expression (by Gal80[ts]) of InR^UAS.cUa, for 4 or 14 days under the control of Scer\GAL4^NP5130, leads to a strong increase in the proportion of mitotic cells in the adult midgut, as compared to controls.

(Wang et al., 2014)

Expression of InR^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF results in substantial overgrowth and disorganisation in the eye, as reflected by loss of ommatidial structure and bristle pattern.

(Buszard et al., 2013)

Overexpression of InR^Scer\UAS.cUa in the mushroom bodies, under the control of Scer\GAL4^ey-OK107 increases the number of mushroom body neurons by 33%. There is a disproportional enlargement of the γ lobe, suggesting InR^Scer\UAS.cUa overexpression causes enhanced cycling in the early larval instar stage, when proliferation rates are normally low.

(Lin et al., 2013)

Expression of InR^Scer\UAS.cUa under the control of Scer\GAL4^kirre.PU does not affect somatic muscle pattern in embryos. The size of the segmental border muscle and the DA1 muscle and also the number of nuclei present in these muscles are normal.

(Tixier et al., 2013)

Expression of InR^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF results in severe overgrowth of the eye.

(Wu et al., 2011)

Expression of InR^UAS.cUa in larvae under the control of Scer\GAL4^repo results in a thickened optic stalk due to increased numbers of glial cells; there is little or no overmigration of glial cells along the Bolwig nerve, compared to controls.

(Witte et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

InR^UAS.cUa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Ptp61F^{UAS.n.Tag:MYC}, Scer\GAL4^GMR.PF

(Buszard et al., 2013)

InR^UAS.cUa, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Ptp61F^{UAS.m.Tag:MYC}, Scer\GAL4^GMR.PF

(Buszard et al., 2013, Wu et al., 2011)

InR^UAS.cUa, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Ptp61F^{UAS.m.Tag:MYC}/dock^UAS.cRa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

NOT suppressed by

Statement

Reference

InR^UAS.cUa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by dock^UAS.cRa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PU, InR^UAS.cUa is an enhancer of visible | adult stage phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

InR^UAS.cUa, Scer\GAL4^elav.PU is an enhancer of abnormal memory | adult stage | progressive phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^elav.PU

(Wang et al., 2012)

Suppressor of

Statement

Reference

InR^UAS.cUa/Scer\GAL4^esg.PU is a suppressor | partially of abnormal developmental rate phenotype of DENR^KO

(Schleich et al., 2014)

Other

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, InR^UAS.cUa, Scer\GAL4^GMR.PU has neoplasia phenotype

(Padash Barmchi et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

InR^UAS.cUa, Scer\GAL4^NP5130 has midgut | adult stage phenotype, suppressible | partially by Xbp1^UAS.RB, Scer\GAL4^NP5130

(Wang et al., 2014)

InR^UAS.cUa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Ptp61F^{UAS.n.Tag:MYC}, Scer\GAL4^GMR.PF

(Buszard et al., 2013)

InR^UAS.cUa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Ptp61F^{UAS.m.Tag:MYC}, Scer\GAL4^GMR.PF

(Buszard et al., 2013, Wu et al., 2011)

InR^UAS.cUa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Ptp61F^{UAS.m.Tag:MYC}/dock^UAS.cRa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

NOT suppressed by

Statement

Reference

InR^UAS.cUa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by dock^UAS.cRa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PU, InR^UAS.cUa is an enhancer of eye phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Suppressor of

Statement

Reference

InR^UAS.cUa/Scer\GAL4^esg.PU is a suppressor | partially of histoblast nest phenotype of DENR^KO

(Schleich et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The increased proportion of mitotic adult midgut cells resulting from the temporally regulated expression (by Gal80[ts]) of InR^UAS.cUa, for 4 or 14 days under the control of Scer\GAL4^NP5130, is partially suppressed by the co-expression of Xbp1^UAS.RB.

(Wang et al., 2014)

Co-expression of Ptp61F^{Scer\UAS.m.T:Hsap\MYC} partially suppresses the eye overgrowth phenotype caused by expression of InR^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

Co-expression of Ptp61F^{Scer\UAS.n.T:Hsap\MYC} completely suppresses the eye overgrowth phenotype caused by expression of InR^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

(Buszard et al., 2013)

Co-expression of Ptp61F^{Scer\UAS.m.T:Hsap\MYC} partially suppresses the eye overgrowth phenotype caused by expression of InR^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

Co-expression of dock^Scer\UAS.cRa does not suppress the eye overgrowth phenotype caused by expression of InR^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

Co-expression of dock^Scer\UAS.cRa results in a further reduction in eye overgrowth in flies co-expressing both Ptp61F^{Scer\UAS.m.T:Hsap\MYC} and InR^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

(Wu et al., 2011)

Xenogenetic Interactions

Statement

Reference

The additional co-expression of InR^Scer\UAS.cUa enhances the fully penetrant rough eye phenotype that results from the co-expression of HPV18\E6^{Scer\UAS.T:Hsap\MYC} and Hsap\UBE3A^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PU, leading to overgrowth and out-pocketing of the eye tissue.

(Padash Barmchi et al., 2016)

The age-progressive memory induced by expression of Hsap\APP^{Aβ1-42.Scer\UAS.cIa} under the control of Scer\GAL4^elav.PU is enhanced by co-expression of InR^Scer\UAS.cUa : the memory defect are detectable even in young 5-day-old flies that perform normally in the aversive olfactory memory assay when expressing Hsap\APP^{Aβ1-42.Scer\UAS.cIa} alone.

(Wang et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

InR^Scer\UAS.cUa

InR^UAS.cUa

InR^wt

(Tixier et al., 2013)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (19)

Report Sections

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