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General Information
Symbol
Dmel\PRL-1UAS.cPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Pagarigan
FlyBase ID
FBal0284665
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a full-length PRL-1 cDNA (LD12894).
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
In adult mechanosensory neurons expressing PRL-1UAS.cPa under the control of Scer\GAL4pnr-MD237, axon terminals appear less mature than controls but do not show significant changes in terminal arbor territory; axons show small protrusions on the main axon branch that show significantly more synapses (Syt1 puncta), and even longer filopodium-like protrusions in terminal region, which are not detected in controls. Posterior dorsocentral bristle neurons of individuals that are both PRL-12/PRL-14 transheterozygous and express PRL-1UAS.cPa under the control of Scer\GAL4pnr-MD237 do not show significant projection defects (terminal arbor territory or number of synapses/ Syt1-positive puncta) in the contralateral branch, as compared to controls.
Expression of PRL-1Scer\UAS.cPa in the posterior compartment of the wing disc under the control of Scer\GAL4en-e16E reduces the surface area of the adult wing by 20%. Expression in the dorsal compartment using Scer\GAL4ap-md544 leads to an upwards curvature, which is also indicative of a decrease in surface area. Expression of PRL-1Scer\UAS.cPa in the developing eye (using Scer\FLP1ey.4x.Exel under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) leads to a smaller eye and head capsule. Wing disc clones expressing PRL-1Scer\UAS.cPa (under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) contain fewer cells than controls. Ubiquitous expression of PRL-1Scer\UAS.cPa under the control of Scer\GAL4Act.PU prevents larval growth; although the larvae consume food, most stall in the first instar larval stages of development for 2-6 days before dying. The larvae do not exhibit any obvious morphological defects. Flies co-expressing PRL-1Scer\UAS.cPa and PRL-1Scer\UAS.NC in the dorsal compartment of the wing disc under the control of Scer\GAL4ap-md544 do not display any wing curvature.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Expression of PRL-1Scer\UAS.cPa partially suppresses the pupal lethality seen when Src64BUY1332 is expressed in the dorsal compartment of the wing disc under the control of Scer\GAL4ap-md544, enabling 45% of the expected adults to eclose. The overgrowth and apoptosis seen in the larval wing discs are also suppressed by PRL-1Scer\UAS.cPa. Expression of PRL-1Scer\UAS.cPa enhances the lethality seen when Ras85DV12.Scer\UAS is expressed in the dorsal compartment of the wing disc under the control of Scer\GAL4ap-md544. Fewer than 10% of mutants reach the prepupal stage, compared to 90% when Ras85DV12.Scer\UAS is expressed alone. The larvae appear lethargic.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PRL-1Scer\UAS.cPa
PRL-1UAS.cPa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Pagarigan
Secondary FlyBase IDs
    References (2)