Open Close
General Information
Symbol
Dmel\AcfUAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0284718
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of Acf tagged with Tag:FLAG.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Constitutive expression of Acf1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4αTub84B.PL is lethal. Overexpression of Acf1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey.PH results in gross abnormalities in the adult eye, ranging from areas that lack ommatidia, the outgrowth of antenna-like structures from the eye field, oversized or deformed eyes and antannal duplication. These phenotypes are highly penetrant regardless of the transgene insertion site, as they are observed 65-77% of the adult flies analysed. About half of the eye imaginal discs show obvious morphological abnormalities and irregularities in photoreceptor cell arrangement upon expression of Acf1Scer\UAS.T:Zzzz\FLAG with the Scer\GAL4ey.PH driver. Overexpression of Acf1Scer\UAS.T:Zzzz\FLAG using Scer\GAL4en.PU or Scer\GAL4da.G32 drivers results in normal adult fly structures. No abnormalities result from the overexpression of Acf1Scer\UAS.T:Zzzz\FLAG in post-mitotic photoreceptor cells posterior to the morphogenetic furrow using the Scer\GAL4GMR.PF driver. Scer\GAL4ey.PH>Acf1Scer\UAS.T:Zzzz\FLAG-expression leads to a delay asynchrony of S phase onset along the morphogenetic furrow. The distribution of apoptotic cells in eye discs expressing Scer\GAL4ey.PH>Acf1Scer\UAS.T:Zzzz\FLAG is not profoundly different from that of controls. However, in some Scer\GAL4ey.PH>Acf1Scer\UAS.T:Zzzz\FLAG discs, but never in controls, apoptotic cells are noticed which are aligned parallel to the morphogenetic furrow. Expression of Acf1Scer\UAS.T:Zzzz\FLAG in salivary gland cells under the control of Scer\GAL42314 probably interferes with heterochromatin formation, because the banding pattern and size of nuclei with polytene chromosomes appear abnormal. Although a certain degree of variability is observed, the nuclei where Acf1Scer\UAS.T:Zzzz\FLAG is expressed appear to be generally larger than in controls.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Acf1Scer\UAS.T:Zzzz\FLAG
AcfScer\UAS.T:Zzzz\FLAG
AcfUAS.Tag:FLAG
Name Synonyms
Secondary FlyBase IDs
    References (1)