FB2025_01 , released February 20, 2025
Allele: Dmel\Vha44UAS.Tag:HA
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General Information
Symbol
Dmel\Vha44UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0284998
Feature type
allele
Associated gene
Dmel\Vha44
Associated Insertion(s)
Carried in Construct
P{UAS-Vha44-HA}
Key Links
Transgenic product class
wild_type
(Petzoldt et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Petzoldt et al., 2013)
Mutagen
in vitro construct
(Petzoldt et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-Vha44-HA}
Cytology
Description

UAS regulatory sequences drive expression of Vha44 which is tagged with a Tag:HA epitope.

(Petzoldt et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Vha44
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Petzoldt et al., 2013)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Vha44Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ptc-559.1 in the wing disc in an anterior stripe at the boundary with the posterior compartment results in a unidirectional migration of these cells into the posterior compartment. It appears that cells close to the boundary are extruded basally and then move towards the posterior compartment. A significant proportion of the cells are positive for apoptotic markers.

      Expression of Vha44Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ap-md544 results in overgrowth of the dorsal compartment of the wing disc.

      Expression of Vha44Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nub.PU results in overgrowth of the wing pouch of the wing disc.

      (Petzoldt et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Ras85DV12.cUa.Scer\UAS increases the extent of migration into the posterior compartment of anterior cells expressing Vha44Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ptc-559.1 in the wing disc.

      Co-expression of Ras85DV12.cUa.Scer\UAS enhances the wing pouch overgrowth caused by expression of Vha44Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nub.PU.

      Co-expression of pucScer\UAS.cMa suppresses the cell invasion phenotype (migration into the posterior compartment) seen in anterior cells in the wing disc expressing Vha44Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ptc-559.1.

      Co-expression of bskDN.Scer\UAS suppresses the wing disc overgrowth caused by expression of Vha44Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ap-md544.

      (Petzoldt et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Vha44Scer\UAS.T:Ivir\HA1
      Vha44UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)