FlyBase Allele Report: Dmel\p53[UAS.Dp53]

General Information

Symbol

Dmel\p53^UAS.Dp53

Species

D. melanogaster

Name

FlyBase ID

FBal0285117

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

PBac{UAS-p53.Dp53}

Also Known As

UAS-Dp53

Key Links

Transgenic product class

wild_type

(Dichtel-Danjoy et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Dichtel-Danjoy et al., 2013)

Mutagen

in vitro construct

(Dichtel-Danjoy et al., 2013)

Progenitor genotype

Carried in construct

PBac{UAS-p53.Dp53}

Cytology

Description

UASt regulatory sequences drive expression of the Dp53 isoform of p53.

(Dichtel-Danjoy et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Scer\GAL4^ninaE.PT

(Robin et al., 2019)

increased cell death, with Scer\GAL4^Bx-MS1096

(Dichtel-Danjoy et al., 2013)

increased cell death, with Scer\GAL4^Bx-MS1096, p53^5A-1-4

(Dichtel-Danjoy et al., 2013)

increased occurrence of cell division | larval stage, with Scer\GAL4^Bx-MS1096

(Dichtel-Danjoy et al., 2013)

visible | adult stage, with Scer\GAL4^en.PU

(Dichtel-Danjoy et al., 2013)

Phenotype Manifest In

autophagosome, with Scer\GAL4^ninaE.PT

(Robin et al., 2019)

eye photoreceptor cell, with Scer\GAL4^ninaE.PT

(Robin et al., 2019)

wing, with Scer\GAL4^en.PU

(Dichtel-Danjoy et al., 2013)

wing disc, with Scer\GAL4^Bx-MS1096

(Dichtel-Danjoy et al., 2013)

Detailed Description

Statement

Reference

Expressing p53^UAS.Dp53 under the control of Scer\GAL4^ninaE.PT does not induce apoptosis (cleaved Dcp1) of photoreceptor cells, despite of their loss in the adult retina, as compared to controls.

The expression of p53^UAS.Dp53 under the control of Scer\GAL4^ninaE.PT induces the formation of autophagosomes (Atg8a puncta and TEM) in the photoreceptor cells of the retina, as compared to controls. There are no apparent defects in autophagic flux ( autophagosome:autolysosome ratio, inferred from the yellow:red ratio of mCherry-GFP-Atg8a puncta).

(Robin et al., 2019)

Expression of p53^{Scer\UAS.Dp53} under the control of Scer\GAL4^en.PU leads to the formation of blisters in the adult wings.

Expression of p53^{Scer\UAS.Dp53} under the control of Scer\GAL4^Bx-MS1096 induces apoptosis in wing discs. A small increase in cell proliferation is seen compared to controls, but less than when p53^{Scer\UAS.DΔNp53} is expressed. An increase in apoptosis is also seen when p53^{Scer\UAS.Dp53} is expressed in a p53^5A-1-4 mutant background.

(Dichtel-Danjoy et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ninaE.PT, p53^UAS.Dp53 has decreased cell number | adult stage phenotype, enhanceable | somatic clone by Atg1^Δ3D/Atg1^Δ3D

(Robin et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^ninaE.PT, p53^UAS.Dp53 has decreased cell number | adult stage phenotype, non-suppressible by BacA\p35^GMR.PH

(Robin et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ninaE.PT, p53^UAS.Dp53 has eye photoreceptor cell phenotype, enhanceable | somatic clone by Atg1^Δ3D/Atg1^Δ3D

(Robin et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^ninaE.PT, p53^UAS.Dp53 has eye photoreceptor cell phenotype, non-suppressible by BacA\p35^GMR.PH

(Robin et al., 2019)

Scer\GAL4^ninaE.PT, p53^UAS.Dp53 has autophagosome phenotype, non-suppressible by BacA\p35^UAS.cUa, Scer\GAL4^ninaE.PT

(Robin et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^nanos.PU/p53^UAS.Dp53 partially rescues p53^E8

(Napoletano et al., 2017)

Comments

The expression of p53^{Scer\UAS.Dp53} under the control of Scer\GAL4^nos.PU suppresses the significant proportion of hyperplastic adult testes in p53^E8 homozygotes, as well as the increased necrosis at their apical tip.

(Napoletano et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

p53^{Scer\UAS.Dp53}

p53^UAS.Dp53

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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