UAS regulatory sequences drive expression of Atx2 tagged at the C-terminal end with 3xTag:FLAG.
The expression of Atx2UAS.Tag:FLAG under the control of Scer\GAL4P2.4.Pdf does not seem to have a major effect on circadian behavior.
Atx2UAS.Tag:FLAG, Scer\GAL4P2.4.Pdf is an enhancer of circadian rhythm defective | adult stage phenotype of Hsap\HTTQ103.ex1p.UAS.EGFP, Scer\GAL4P2.4.Pdf
Scer\GAL4cry.PE/Atx2UAS.Tag:FLAG is a non-suppressor of locomotor rhythm defective | adult stage phenotype of Lsm12aΔ6
Atx2UAS.Tag:FLAG, Hsap\HTTQ25.ex1p.UAS.EGFP, Scer\GAL4P2.4.Pdf has circadian rhythm defective | adult stage phenotype
The aberrant long-period circadian locomotor rhythm under free-running constant dark conditions characteristic for Lsm12Δ6 mutant adults cannot be rescued by Scer\GAL4cry.PE-driven expression of Atx2Scer\UAS.T:Zzzz\FLAG.
Co-expressing Hsap\HTTQ25.ex1p.UAS.EGFP and Atx2UAS.Tag:FLAG under the control of Scer\GAL4P2.4.Pdf, severely impairs circadian behavior, as compared to Hsap\HTTQ25.ex1p.UAS.EGFP and wild-type controls.