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General Information
Symbol
Dmel\Ras85DR68Q
Species
D. melanogaster
Name
FlyBase ID
FBal0285348
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ras1R68Q
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G9511933A

Reported nucleotide change:

G641A

Amino acid change:

R68Q | Ras85D-PA

Reported amino acid change:

R68Q

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: R68Q.

Nucleotide substitution: G641A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies mutant for Ras85DR68Q exhibit a number of developmental defects that are characteristic of abnormally elevated RTK/RAS/MAPK signaling, including enhanced resistance to apoptosis, supernumerary R7 cells in the eye and ectopic wing vein formation.

Ras85DR68Q mutants have a reduced basal level of GTPase activity.

Ras85DR68Q mutants exhibit a statistically significant increase in the number of midline glia cells at stage 17 of embryogenesis, compared to controls.

Ras85DR68Q mutants exhibit supernumerary R7 cells in the developing ommatidia. Occasionally, ommatidia are found without outer photoreceptor cells, a phenotypic consequence of elevated MAPK signaling.

Ras85DR68Q flies show abnormalities in adult wing tissues. Homozygous Ras85DR68Q flies have an extra longitudinal 'veinlet' branching off the posterior crossvein. Additionally, an ectopic longitudinal vein is seen beneath the posterior crossvein and there is an ectopic crossvein found between the L4 and L5 wing veins near the hinge.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference

Ras85DR68Q has wing vein | ectopic phenotype, suppressible by rl10a

Ras85DR68Q has eye phenotype, suppressible by rl10a

Ras85DR68Q has ommatidium phenotype, suppressible by rl10a

Ras85DR68Q has wing phenotype, suppressible by rl10a

Ras85DR68Q has crossvein phenotype, suppressible by rl10a

Ras85DR68Q has wing vein L4 phenotype, suppressible by rl10a

Ras85DR68Q has wing vein L5 phenotype, suppressible by rl10a

NOT suppressed by
Statement
Reference

Ras85DR68Q has wing vein | ectopic phenotype, non-suppressible by Egfrf2

Ras85DR68Q has wing vein | ectopic phenotype, non-suppressible by aosΔ7

Ras85DR68Q has eye phenotype, non-suppressible by aosΔ7

Ras85DR68Q has midline glial cell phenotype, non-suppressible by aosΔ7

Ras85DR68Q has ommatidium phenotype, non-suppressible by aosΔ7

Ras85DR68Q has wing phenotype, non-suppressible by aosΔ7

Ras85DR68Q has crossvein phenotype, non-suppressible by aosΔ7

Ras85DR68Q has wing vein L4 phenotype, non-suppressible by aosΔ7

Ras85DR68Q has eye phenotype, non-suppressible by Egfrf2

Ras85DR68Q has wing vein L5 phenotype, non-suppressible by aosΔ7

Ras85DR68Q has midline glial cell phenotype, non-suppressible by Egfrf2

Ras85DR68Q has ommatidium phenotype, non-suppressible by Egfrf2

Ras85DR68Q has wing phenotype, non-suppressible by Egfrf2

Ras85DR68Q has crossvein phenotype, non-suppressible by Egfrf2

Ras85DR68Q has wing vein L4 phenotype, non-suppressible by Egfrf2

Ras85DR68Q has wing vein L5 phenotype, non-suppressible by Egfrf2

Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Ras85DR68Q suppresses WGMR.PG induced cell death in a dosage-dependent manner.

Ras85DR68Q suppresses WAla3.GMR (which lacks 3 of the 5 predicted MAPK phosphorylation sites) induced cell death.

Ras85DR68Q fails to suppress WAla5.GMR (which lacks all of the 5 predicted MAPK phosphorylation sites) induced cell death.

Ras85DR68Q very mildly suppresses grimGMR.PC and rprGMR.PW induced cell death although this is suspected to be due to suppression of endogenous W activity and not grim or rpr activity.

A Ras85DR68Q background can partially suppress the hemocyte death seen upon expression of WScer\UAS.cZa under the control of Scer\GAL4Hml.PG. Hemocytes are clearly visible anteriorly in the lymph glands of 3rd instar larvae. Circulating hemocytes, however, appear to remain susceptible to W-induced cell death and are missing, even in the presence of two copies of Ras85DR68Q.

An Egfrf2 background does not affect the developmental and apoptotic defects found in Ras85DR68Q mutants.

A aosΔ7 background does not affect the developmental and apoptotic defects found in Ras85DR68Q mutants.

A rl10a background strongly ameliorates the developmental and apoptotic defects found in Ras85DR68Q mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Ras85DN17.sev expression in the eye abrogates the suppressive effects of Ras85DR68Q on WGMR.PG-induced apoptosis.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)