FlyBase Allele Report: Dmel\Abl[UAS.cHa]

General Information

Symbol

Dmel\Abl^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hsouna

FlyBase ID

FBal0285360

Feature type

allele

Associated gene

Dmel\Abl

Associated Insertion(s)

Carried in Construct

P{UAS-Abl.H}

Also Known As

UAS-Ablwt

Key Links

Transgenic product class

cDNA

(Hsouna et al., 2003)

wild_type

(Hsouna et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hsouna et al., 2003)

wild_type

(Hsouna et al., 2003)

Mutagen

in vitro construct

(Hsouna et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Abl.H}

Cytology

Description

UASt regulatory sequences drive expression of a wild-type Abl cDNA.

(Hsouna et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Abl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage 16, with Scer\GAL4^elav.PLu

(Hsouna et al., 2003)

abnormal neuroanatomy | embryonic stage 16, with Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

Phenotype Manifest In

larval longitudinal connective | embryonic stage 16, with Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

Detailed Description

Statement

Reference

Commissures in embryos expressing Scer\GAL4^insc-Mz1407>Abl^Scer\UAS.cHa are similar to that of wild-type.

(Dorsten et al., 2010)

Overexpression of Abl^Scer\UAS.cHa by Scer\GAL4^elav.PLu or Scer\GAL4^ftz.ng results in occasional abnormal crossovers in less than 14% of embryos examined.

Co-expression of Abl^Scer\UAS.cHa and Abl^KN.Scer\UAS in the Scer\GAL4^ftz.ng pattern suppresses all abnormal axonal crossovers.

(Hsouna et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/Abl^UAS.cHa is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of fra³/fra⁴

(Dorsten et al., 2010)

Abl^UAS.cHa/Scer\GAL4^ftz.ng is an enhancer of abnormal neuroanatomy | embryonic stage 16 | dominant phenotype of robo1¹

(Hsouna et al., 2003)

Abl^UAS.cHa/Scer\GAL4^elav.PLu is an enhancer of abnormal neuroanatomy | embryonic stage 16 | dominant phenotype of sli¹

(Hsouna et al., 2003)

Suppressor of

Statement

Reference

Abl^UAS.cHa/Scer\GAL4^ftz.ng is a suppressor of abnormal neuroanatomy phenotype of comm¹

(Hsouna et al., 2003)

Abl^UAS.cHa/Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of comm¹

(Hsouna et al., 2003)

Other

Statement

Reference

Abl^UAS.cHa, Scer\GAL4^insc-Mz1407, fra³/fra⁴ has abnormal neuroanatomy | embryonic stage phenotype

(Dorsten et al., 2010)

Abl^UAS.cHa, Scer\GAL4^insc-Mz1407, fra³/fra[+] has abnormal neuroanatomy | embryonic stage phenotype

(Dorsten et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/Abl^UAS.cHa is an enhancer of larval ventral nerve cord commissure phenotype of fra³/fra⁴

(Dorsten et al., 2010)

Abl^UAS.cHa/Scer\GAL4^ftz.ng is an enhancer of larval longitudinal connective | embryonic stage 16 phenotype of robo1¹

(Hsouna et al., 2003)

Abl^UAS.cHa/Scer\GAL4^elav.PLu is an enhancer of larval longitudinal connective | embryonic stage 16 phenotype of sli¹

(Hsouna et al., 2003)

Suppressor of

Statement

Reference

Abl^UAS.cHa/Scer\GAL4^ftz.ng is a suppressor of symmetrical commissure phenotype of comm¹

(Hsouna et al., 2003)

Abl^UAS.cHa/Scer\GAL4^elav.PLu is a suppressor of symmetrical commissure phenotype of comm¹

(Hsouna et al., 2003)

Other

Statement

Reference

Abl^UAS.cHa, Scer\GAL4^insc-Mz1407, fra³/fra⁴ has tract | ectopic | embryonic stage phenotype

(Dorsten et al., 2010)

Abl^UAS.cHa, Scer\GAL4^insc-Mz1407, fra³/fra[+] has larval ventral nerve cord commissure phenotype

(Dorsten et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Over-expression of Abl^Scer\UAS.cHa using Scer\GAL4^insc-Mz1407 enhances the degree of thinning and missing posterior commissures in fra³/fra⁴ embryos, and many anterior commissures disappear. Large bundles of axons are observed ectopically exiting the central nervous system (CNS), often extending beyond the CNS/PNS boundary.

A rare commissure may be malformed in heterozygous fra³ embryos expressing Scer\GAL4^insc-Mz1407>Abl^Scer\UAS.cHa.

Re-expression of fra^Scer\UAS.cKa in fra³/fra⁴ mutants reverts the phenotype back to that seen when Abl^Scer\UAS.cHa is expressed alone under the control of Scer\GAL4^insc-Mz1407 : normal anterior and posterior commissure formation.

Commissure formation is restored in fra³/fra⁴ mutants expressing both fra^{ΔP1.Scer\UAS} and Abl^Scer\UAS.cHa under the control of Scer\GAL4^insc-Mz1407.

Commissure formation is restored in fra³/fra⁴ mutants expressing both fra^{ΔP2.Scer\UAS} and Abl^Scer\UAS.cHa under the control of Scer\GAL4^insc-Mz1407.

Commissure formation is restored in fra³/fra⁴ mutants expressing both fra^{ΔP3.Scer\UAS} and Abl^Scer\UAS.cHa under the control of Scer\GAL4^insc-Mz1407.

Nearly a third of hemi-segments in fra³/fra⁴ embryos expressing Scer\GAL4^insc-Mz1407>Abl^Scer\UAS.cHa exhibit defects characterised by Fas2-positive axons ectopically exiting the central nervous system (CNS).

(Dorsten et al., 2010)

Compared with heterozygous robo¹ mutants alone, overexpression of Abl^Scer\UAS.cHa using Scer\GAL4^ftz.ng enhances the frequency of abnormal axonal crossovers.

Compared with heterozygous sli¹ mutants alone, pan-neural overexpression of Abl^Scer\UAS.cHa using Scer\GAL4^elav.PLu enhances the frequency of abnormal axonal crossovers. Scer\GAL4^elav.PLu>Abl^Scer\UAS.cHa expression causes axons to cross the midline inappropriately in most segments of all embryos in sli¹ heterozygous mutants.

Using Scer\GAL4^ftz.ng, expression of Abl^Scer\UAS.cHa in a subset of neurons in a comm¹ background causes a few thin commissures to reform.

Pan-neural expression of Abl^Scer\UAS.cHa under the control of Scer\GAL4^elav.PLu partially suppresses the comm¹ commissure phenotype.

(Hsouna et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Abl^Scer\UAS.cHa

Abl^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hsouna

Secondary FlyBase IDs

References (2)

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