FlyBase Allele Report: Dmel\robo1::robo2[R2I1+2.UAS.Tag:HA,Tag:SS(wg)]

General Information

Symbol

Dmel\robo1::robo2^{R2I1+2.UAS.Tag:HA,Tag:SS(wg)}

Species

D. melanogaster

Name

FlyBase ID

FBal0285367

Feature type

allele

Associated gene

robo1::robo2

Associated Insertion(s)

Carried in Construct

P{UAS-robo1::robo2.R2I1+2.HA}

Also Known As

UAS-Robo1^R2I1+2

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Evans and Bashaw, 2010)

Progenitor genotype

Carried in construct

P{UAS-robo1::robo2.R2I1+2.HA}

Cytology

Description

UASt regulatory sequences drive expression of a chimeric robo1::robo2 protein (composed of robo2 amino-acid residues 84-280 and robo1 amino acid residues 253-1395) fused with a Tag:SS(wg) signal sequence and an N-terminal Tag:HA-tag.

(Evans and Bashaw, 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PLu

(Evans and Bashaw, 2010)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^unspecified

(Evans and Bashaw, 2010)

Phenotype Manifest In

larval longitudinal connective, with Scer\GAL4^ap-md544

(Evans and Bashaw, 2010)

larval longitudinal connective, with Scer\GAL4^elav.PLu

(Evans and Bashaw, 2010)

larval longitudinal connective, with Scer\GAL4^unspecified

(Evans and Bashaw, 2010)

tract | ectopic | embryonic stage, with Scer\GAL4^elav.PLu

(Evans and Bashaw, 2010)

Detailed Description

Statement

Reference

Mis-expression of robo::lea^{R2I1+2.Scer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu results in thickened commissures and promotes ectopic midline crossing of Fas2-positive axon tracts.

(Evans and Bashaw, 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

robo1::robo2^{R2I1+2.UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of comm^Δe39

(Evans et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

robo1::robo2^{R2I1+2.UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu is a suppressor of symmetrical commissure phenotype of comm^Δe39

(Evans et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of robo1::robo2^{R2I1+2.Scer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu strongly suppresses the loss of commissure phenotype of homozygous comm^E39 embryos.

(Evans et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

robo1::robo2^{R2I1+2.Scer\UAS.T:Ivir\HA1,T:SS-wg}

robo1::robo2^{R2I1+2.UAS.Tag:HA,Tag:SS(wg)}

robo::lea^{R2I1+2.Scer\UAS.T:Ivir\HA1,T:SS-wg}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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