FlyBase Allele Report: Dmel\SPARC[UASp.cPa]

General Information

Symbol

Dmel\SPARC^UASp.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Portela

FlyBase ID

FBal0285800

Feature type

allele

Associated gene

Dmel\SPARC

Associated Insertion(s)

Carried in Construct

P{UASp-SPARC.P}

Also Known As

UAS-SPARC

Key Links

Transgenic product class

wild_type

(Portela et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Portela et al., 2010)

Mutagen

in vitro construct

(Portela et al., 2010)

Progenitor genotype

Carried in construct

P{UASp-SPARC.P}

Cytology

Description

UASp regulatory sequences drive expression of SPARC.

(Portela et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

SPARC

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death | pupal stage, with Scer\GAL4^elav.PU

(Merino et al., 2013)

decreased cell death | pupal stage, with Scer\GAL4^GMR.PF

(Merino et al., 2013)

Phenotype Manifest In

photoreceptor neuron | pupal stage, with Scer\GAL4^elav.PU

(Merino et al., 2013)

photoreceptor neuron | pupal stage, with Scer\GAL4^GMR.PF

(Merino et al., 2013)

Detailed Description

Statement

Reference

Expression of BM-40-SPARC^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF significantly reduces the physiological peripheral photoreceptor cell death seen in control pupae.

Expression of BM-40-SPARC^Scer\UAS.cPa in photoreceptor neurons under the control of Scer\GAL4^elav.PU suppresses the physiological cell death seen at the periphery of the pupal retina.

Expressing BM-40-SPARC^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF (but restricting expression in the neuronal cells using Scer\GAL80^elav.PU) does not suppress the physiological cell death seen at the periphery of the pupal retina.

Expressing BM-40-SPARC^Scer\UAS.cPa in primary pigment cells and cone cells under the control of Scer\GAL4^wg.PU does not suppress the physiological cell death seen at the periphery of the pupal retina.

(Merino et al., 2013)

The overexpression of BM-40-SPARC^Scer\UAS.cPa in the genital disc using Scer\GAL4^GMR.PF gives rise to wild-type terminalia.

(Portela et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

SPARC^UASp.cPa, Scer\GAL4^Act.PU is a suppressor of abnormal size | somatic clone | wandering third instar larval stage phenotype of Scer\GAL4^Act.PU, wupA^JF02172

(Casas-Tintó et al., 2016)

SPARC^UASp.cPa, Scer\GAL4^Act.PU is a suppressor of increased cell death | somatic clone | wandering third instar larval stage phenotype of Scer\GAL4^Act.PU, wupA^JF02172

(Casas-Tintó et al., 2016)

SPARC^UASp.cPa, Scer\GAL4^en.PU is a suppressor of increased cell death | wandering third instar larval stage phenotype of Scer\GAL4^en.PU, wupA^JF02172

(Casas-Tintó et al., 2016)

SPARC^UASp.cPa/Scer\GAL4^αTub84B.PBb is a suppressor of increased cell death | somatic clone | cell non-autonomous phenotype of Myc^αTub84B.PBb

(Portela et al., 2010)

NOT Suppressor of

Statement

Reference

SPARC^UASp.cPa/Scer\GAL4^ap-md544 is a non-suppressor of increased cell death | dominant | third instar larval stage phenotype of ap^md544

(Portela et al., 2010)

SPARC^UASp.cPa, Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Portela et al., 2010)

SPARC^UASp.cPa, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Portela et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

SPARC^UASp.cPa, Scer\GAL4^Act.PU is a suppressor of wing disc | somatic clone | wandering third instar larval stage phenotype of Scer\GAL4^Act.PU, wupA^JF02172

(Casas-Tintó et al., 2016)

SPARC^UASp.cPa, Scer\GAL4^en.PU is a suppressor of wing disc posterior compartment | wandering third instar larval stage phenotype of Scer\GAL4^en.PU, wupA^JF02172

(Casas-Tintó et al., 2016)

NOT Suppressor of

Statement

Reference

SPARC^UASp.cPa/Scer\GAL4^ap-md544 is a non-suppressor of wing disc | third instar larval stage phenotype of ap^md544

(Portela et al., 2010)

SPARC^UASp.cPa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Portela et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

In wandering third instar larvae, the co-expression of SPARC^Scer\UAS.cPa suppresses the decreased relative area and apoptosis in somatic wing disc clones expressing wupA^JF02172 under the control of Scer\GAL4^Act.PU and the increased apoptosis in posterior wing disc compartments expressing wupA^JF02172 under the control of Scer\GAL4^en.PU.

(Casas-Tintó et al., 2016)

Overexpression of BM-40-SPARC^Scer\UAS.cPa under the control of Scer\GAL4^ap-md544 fails to suppress the increased apoptosis in ap^md544/+ wing discs.

When BM-40-SPARC^Scer\UAS.cPa is activated specifically in the loser wild-type cells using Scer\GAL4^αTub84B.PBb surrounded by dm^αTub84B.PBb-expressing super-competitor cells, apoptosis is reduced and cell competition is prevented.

Co-expression of BM-40-SPARC^Scer\UAS.cPa fails to suppress the eye-size defect resulting from the ectopic expression of egr^Scer\UAS.cMa using Scer\GAL4^GMR.PF.

(Portela et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

BM-40-SPARC^Scer\UAS.cPa

SPARC^Scer\UAS.cPa

SPARC^UASp.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Portela

Secondary FlyBase IDs

References (5)

Report Sections

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