FlyBase Allele Report: Dmel\Optix[FL.UAS]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\Optix^FL.UAS

General Information

Symbol

Dmel\Optix^FL.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0285828

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{UAS-Optix.FL}

Also Known As

UAS-Optix

Key Links

Transgenic product class

(Weasner et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Weasner et al., 2007)

Mutagen

in vitro construct

(Weasner et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Optix.FL}

Cytology

Description

UASt regulates expression of full-length Optix.

(Weasner et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | late third instar larval stage, with Scer\GAL4^C855a

(Gold and Brand, 2014)

abnormal cell adhesion | late third instar larval stage, with Scer\GAL4^NP2631

(Gold and Brand, 2014)

abnormal cell adhesion | late third instar larval stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Gold and Brand, 2014)

abnormal cell polarity | late third instar larval stage, with Scer\GAL4^C855a

(Gold and Brand, 2014)

abnormal cell polarity | late third instar larval stage, with Scer\GAL4^NP2631

(Gold and Brand, 2014)

abnormal cell shape | late third instar larval stage, with Scer\GAL4^C855a

(Gold and Brand, 2014)

abnormal cell shape | late third instar larval stage, with Scer\GAL4^NP2631

(Gold and Brand, 2014)

abnormal cell shape | late third instar larval stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Gold and Brand, 2014)

abnormal neuroanatomy | late third instar larval stage, with Scer\GAL4^C855a

(Gold and Brand, 2014)

abnormal neuroanatomy | late third instar larval stage, with Scer\GAL4^NP2631

(Gold and Brand, 2014)

abnormal neuroanatomy | late third instar larval stage, with Scer\GAL4^Vsx1-MzVum

(Gold and Brand, 2014)

abnormal neuroanatomy | late third instar larval stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Gold and Brand, 2014)

visible, with Scer\GAL4^ey.PU

(Anderson et al., 2012)

visible, with Scer\GAL4^GMR.PU

(Anderson et al., 2012)

Phenotype Manifest In

adult head, with Scer\GAL4^c309

(Weasner et al., 2007)

adult head, with Scer\GAL4^cb49

(Weasner et al., 2007)

antenna, with Scer\GAL4^c309

(Weasner et al., 2007)

antenna, with Scer\GAL4^cb49

(Weasner et al., 2007)

antenna, with Scer\GAL4^dpp.PU

(Weasner et al., 2007)

antenna, with Scer\GAL4^sca-C253

(Weasner et al., 2007)

eye, with Scer\GAL4^ey.PU

(Anderson et al., 2012, Weasner and Kumar, 2009, Weasner et al., 2007)

eye, with Scer\GAL4^GMR.PU

(Anderson et al., 2012, Weasner and Kumar, 2009, Weasner et al., 2007)

eye | ectopic, with Scer\GAL4^c309

(Weasner et al., 2007)

eye | ectopic, with Scer\GAL4^cb49

(Weasner et al., 2007)

eye | ectopic, with Scer\GAL4^dpp.PU

(Weasner et al., 2007)

eye | ectopic, with Scer\GAL4^rn-GAL4-5

(Weasner et al., 2007)

eye | ectopic, with Scer\GAL4^sca-C253

(Weasner et al., 2007)

eye disc | late third instar larval stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Gold and Brand, 2014)

eye photoreceptor cell, with Scer\GAL4^ey.PU

(Weasner et al., 2007)

eye photoreceptor cell, with Scer\GAL4^GMR.PU

(Weasner et al., 2007)

haltere, with Scer\GAL4^rn-GAL4-5

(Weasner et al., 2007)

larval outer optic anlage | late third instar larval stage, with Scer\GAL4^C855a

(Gold and Brand, 2014)

larval outer optic anlage | late third instar larval stage, with Scer\GAL4^NP2631

(Gold and Brand, 2014)

larval outer optic anlage | late third instar larval stage, with Scer\GAL4^Vsx1-MzVum

(Gold and Brand, 2014)

larval outer optic anlage | late third instar larval stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Gold and Brand, 2014)

leg disc | late third instar larval stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Gold and Brand, 2014)

optic lobe neuroepithelial cell | late third instar larval stage, with Scer\GAL4^C855a

(Gold and Brand, 2014)

optic lobe neuroepithelial cell | late third instar larval stage, with Scer\GAL4^NP2631

(Gold and Brand, 2014)

optic lobe neuroepithelial cell | late third instar larval stage, with Scer\GAL4^Vsx1-MzVum

(Gold and Brand, 2014)

optic lobe neuroepithelial cell | late third instar larval stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Gold and Brand, 2014)

wing, with Scer\GAL4^rn-GAL4-5

(Weasner et al., 2007)

Detailed Description

Statement

Reference

The morphology of the optic lobe neuroepithelium is distorted in late third instar larvae expressing Optix^FL.UAS under the control of Scer\GAL4^Vsx1-MzVum.

Expressing Optix^FL.UAS under the control of either Scer\GAL4^C855a or Scer\GAL4^NP2631 leads to the formation of a multi-layered neuroepithelium with rosettes of rounded cells which have lost columnar apico-basal polarity in the outer optic anlage of late third instar larvae. GAL80ts was used to induce Optix^FL.UAS expression between 24 and 48 hours after larval hatching. The same phenotype is observed in the outer optic anlage, eye discs and leg discs of late third instar larvae following the induction of somatic clones expressing Optix^FL.UAS under the control of Scer\GAL4^αTub84B.PL.

(Gold and Brand, 2014)

Expression of Optix^FL.Scer\UAS under the control of Scer\GAL4^ey.PU results in a small rough eye.

(Anderson et al., 2012)

Scer\GAL4^GMR.PU-mediated expression of Optix^FL.Scer\UAS drastically inhibits eye development.

Scer\GAL4^ey.PU-mediated expression of Optix^FL.Scer\UAS results in dramatic reductions in eye formation.

(Weasner and Kumar, 2009)

Expression of Optix^FL.Scer\UAS via Scer\GAL4^dpp.PU, Scer\GAL4^rn-GAL4-5, Scer\GAL4^cb49, Scer\GAL4^c309 or Scer\GAL4^sca-C253 yields ectopic eyes on the adult antenna, the ventral portion of the head, or the wing and haltere.

Expression of Optix^FL.Scer\UAS via Scer\GAL4^rn-GAL4-5 results in replacement of wing tissue with retinal tissue.

Expression of Optix^FL.Scer\UAS via Scer\GAL4^ey.PU or Scer\GAL4^GMR.PU results in a severely altered eye external surface and a complete loss of photoreceptor neurons.

(Weasner et al., 2007)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Optix^FL.UAS/Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of so¹

(Weasner and Kumar, 2009, Weasner et al., 2007)

Optix^FL.UAS/Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of so^D

(Weasner et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Optix^FL.Scer\UAS

Optix^FL.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections