FlyBase Allele Report: Dmel\dco[K38R.UAS.Tag:MYC]

General Information

Symbol

Dmel\dco^{K38R.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0285854

Feature type

allele

Associated gene

Dmel\dco

Associated Insertion(s)

Carried in Construct

P{UAS-dco.K38R.MYC}

Also Known As

UAS-DBT^K/R

Key Links

Transgenic product class

dominant_negative_variant

(Muskus et al., 2007)

missense_variant

(Muskus et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Muskus et al., 2007)

missense_variant

(Muskus et al., 2007)

Mutagen

in vitro construct

(Muskus et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-dco.K38R.MYC}

Cytology

Description

UASt drives expression of dco containing a K38R substitution that acts as a dominant negative mutant. It is tagged with Tag:MYC.

(Muskus et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dco

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Means et al., 2015)

exacerbates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^P525L.UAS

(Kishino et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage, with Scer\GAL4^tim.PE

(Wang et al., 2022)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Fan et al., 2013, Muskus et al., 2007)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^tim.PE

(Wang et al., 2022)

abnormal sleep | adult stage, with Scer\GAL4^dco-2A-GAL4

(Wang et al., 2022)

abnormal sleep | adult stage, with Scer\GAL4^tim.PE

(Wang et al., 2022)

decreased sleep | adult stage, with Scer\GAL4^dco-2A-GAL4

(Wang et al., 2022)

decreased sleep | adult stage, with Scer\GAL4^tim.PE

(Wang et al., 2022)

increased cell death | progressive, with Scer\GAL4^tim.PE

(Means et al., 2015)

partially lethal - majority die, with Scer\GAL4^Act5C.PI

(Muskus et al., 2007)

viable, with Scer\GAL4^tim.PE

(Muskus et al., 2007)

Phenotype Manifest In

head, with Scer\GAL4^tim.PE

(Means et al., 2015)

Detailed Description

Statement

Reference

Flies expressing Scer\GAL4^tim.PE>dco^{K38R.Scer\UAS.T:Hsap\MYC} show circadian-dependent activation of apoptosis at an earlier age than do wild-type flies.

(Means et al., 2015)

Scer\GAL4^tim.PE-mediated expression of dco^{K38R.Scer\UAS.T:Hsap\MYC} results in arrhythmic locomotor activity in constant darkness.

(Fan et al., 2013)

Scer\GAL4^Act5C.PI-mediated expression of dco^{K38R.Scer\UAS.T:Hsap\MYC} results in high lethality.

Scer\GAL4^tim.PE, dco^{K38R.Scer\UAS.T:Hsap\MYC} flies are viable with normal eyes, but exhibit high arrhythmicity and/or variably long circadian periods compared to controls.

(Muskus et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^tim.PE, dco^{K38R.UAS.Tag:MYC} has decreased sleep | adult stage phenotype, suppressible by per⁰¹

(Wang et al., 2022)

Enhancer of

Statement

Reference

dco^{K38R.UAS.Tag:MYC}, Scer\GAL4^tim.PE is an enhancer of abnormal locomotor rhythm phenotype of Bdbt^KK102995, Scer\GAL4^tim.PE

(Fan et al., 2013)

Other

Statement

Reference

Hsap\MAPT^GMR.Ex.PJ, Scer\GAL4^GMR.PU, dco^{K38R.UAS.Tag:MYC} has abnormal size phenotype

(Means et al., 2015)

Bdbt^KK102995, Scer\GAL4^tim.PE, dco^{K38R.UAS.Tag:MYC} has short lived phenotype

(Fan et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

dco^{K38R.UAS.Tag:MYC}/Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Means et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dco^{K38R.Scer\UAS.T:Hsap\MYC} worsens the locomotor rhythm defects of Bdbt^KK102995 Scer\GAL4^tim.PE animals in constant darkness. Co-expression of dco^{K38R.Scer\UAS.T:Hsap\MYC} and Bdbt^KK102995 via Scer\GAL4^tim.PE results in a shortened lifespan.

(Fan et al., 2013)

Xenogenetic Interactions

Statement

Reference

When Scer\GAL4^GMR.PU>dco^{K38R.Scer\UAS.T:Hsap\MYC} is expressed along with Hsap\MAPT^GMR.Ex.PJ, there is significantly increased disruption in the eye. The enhanced disruption of the eye is manifested by the appearance of melanized patches in the eye and a decreased average surface area of the eye.

(Means et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

80981

w¹¹¹⁸; P{UAS-dco.K38R.MYC}22F

80980

y¹ w^*; P{UAS-dco.K38R.MYC}13F1A/TM3, Sb¹ Ser¹