FlyBase Allele Report: Dmel\disco[dsRNA.UAS]

General Information

Symbol

Dmel\disco^dsRNA.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0285950

Feature type

allele

Associated gene

Dmel\disco

Associated Insertion(s)

Carried in Construct

P{UAS-disco.RNAi}

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Dey et al., 2009)

in vitro construct - RNAi

(Dey et al., 2009)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-disco.RNAi}

Cytology

Nature of the lesion

Statement

Reference

UAS regulates expression of three different fragments of the disco ORF (1-256bp, 607-927bp, and 1066-1515bp) that target disco RNA for RNAi-mediated degradation.

(Dey et al., 2009)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

disco

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

homeotic | temperature conditional, with Scer\GAL4^Dll-md23

(Dey et al., 2009)

lethal, with Scer\GAL4^αTub84B.PL

(Dey et al., 2009)

partially lethal | temperature conditional, with Scer\GAL4^Dll-md23

(Dey et al., 2009)

some die during larval stage, with Scer\GAL4^αTub84B.PL

(Dey et al., 2009)

some die during P-stage, with Scer\GAL4^αTub84B.PL

(Dey et al., 2009)

Phenotype Manifest In

antenna, with Scer\GAL4^Dll-md23

(Dey et al., 2009)

arista | temperature conditional, with Scer\GAL4^Dll-md23

(Dey et al., 2009)

leg, with Scer\GAL4^Dll-md23

(Dey et al., 2009)

tarsal segment | temperature conditional, with Scer\GAL4^Dll-md23

(Dey et al., 2009)

third segment of antenna | temperature conditional, with Scer\GAL4^Dll-md23

(Dey et al., 2009)

unguis | temperature conditional, with Scer\GAL4^Dll-md23

(Dey et al., 2009)

Detailed Description

Statement

Reference

Expression of disco^{dsRNA.Scer\UAS} using Scer\GAL4^αTub84B.PL causes lethality at both larval and pupal (but not embryonic) stages.

Expression of disco^{dsRNA.Scer\UAS} using Scer\GAL4^Dll-md23 causes some lethality but allows many flies to survive. At 25[o]C, ~64% of animals survive; all escapers show antenna phenotypes characterized by shortening of the central core and deletion of most of the laterals; leg development is also defective but only 10% show deletion of the distal tarsal segments and/or the claw. When raised at 29[o]C for 4-5 days and then transferred to 25[o]C, the survival rate decreases to ~29%; now, over 70% escapers display antenna to leg transformations and the remainder display partial truncation of antennal segment 3 and arista deletion; the leg phenotype is also more severe with ~16% of escapers displaying deletion of tarsal segments 3-5 and the claw.

Expression of disco^{dsRNA.Scer\UAS} using Scer\GAL4^Dll-md23 does not induce cell death in antennal or leg discs.

(Dey et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

disco^dsRNA.UAS/Scer\GAL4^Dll-md23 is an enhancer of lethal phenotype of Dll³/Dll^md23

(Dey et al., 2009)

disco^dsRNA.UAS/Scer\GAL4^Dll-md23 is an enhancer of lethal phenotype of Dll⁹/Dll^md23

(Dey et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

disco^{dsRNA.Scer\UAS}

disco^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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