FlyBase Allele Report: Dmel\Dscam1[RNAi.UAS.18]

General Information

Symbol

Dmel\Dscam1^RNAi.UAS.18

Species

D. melanogaster

Name

FlyBase ID

FBal0286282

Feature type

allele

Associated gene

Dmel\Dscam1

Associated Insertion(s)

Carried in Construct

P{UAS-Dscam1.18.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Shi et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Shi et al., 2007)

Mutagen

in vitro construct

(Shi et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Dscam1.18.RNAi}

Cytology

Description

UAS regulates expression of a microRNA carrying a unique Dscam1 sequence derived from exon 18 (a common Dscam1 exon).

(Shi et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Dscam1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Shi et al., 2007)

abnormal neuroanatomy | adult stage | somatic clone, with Scer\GAL4^GH146

(Shi et al., 2007)

abnormal neuroanatomy | larval stage, with Scer\GAL4^ey-OK107

(Yu et al., 2009)

Phenotype Manifest In

adult antennal lobe, with Scer\GAL4^GH146

(Shi et al., 2007)

adult antennal lobe projection neuron vPN, with Scer\GAL4^GH146

(Shi et al., 2007)

adult lateral horn, with Scer\GAL4^GH146

(Shi et al., 2007)

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

(Shi et al., 2007)

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

(Shi et al., 2007)

embryonic/larval mushroom body, with Scer\GAL4^ey-OK107

(Yu et al., 2009)

Detailed Description

Statement

Reference

Scer\GAL4^ey-OK107-mediated expression of Dscam1^{miRNA.Scer\UAS.18} causes defects in larval mushroom body lobe morphology: 88% of MBs have one huge and one skinny lobe.

Scer\GAL4^tub.PU-mediated expression of Dscam1^{miRNA.Scer\UAS.18} fails to disrupt the ladder-like neuronal tracts in the embryonic ventral ganglion.

(Yu et al., 2009)

Major neural structures of the larval CNS are unaffected by Scer\GAL4^ase.neuro/Scer\GAL4^elav-C155-mediated expression of Dscam^{miRNA.Scer\UAS.18}.

Scer\GAL4^ey-OK107-mediated expression of Dscam^{miRNA.Scer\UAS.18} severely disrupts the formation of the alpha/beta lobes of the adult mushroom body.

Scer\GAL4^GH146-mediated expression of Dscam^{miRNA.Scer\UAS.18} in vPN neuroblast clones potently suppresses the elaboration of dendrites in the antennal lobes and disrupts their axonal arborization in the lateral horns.

(Shi et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Abl²/Abl⁴, Dscam1^RNAi.UAS.18 has abnormal neuroanatomy | embryonic stage phenotype

(Yu et al., 2009)

Phenotype Manifest In

Other

Statement

Reference

Abl²/Abl⁴, Dscam1^RNAi.UAS.18 has larval ventral nerve cord | embryonic stage phenotype

(Yu et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^tub.PU-mediated expression of Dscam1^{miRNA.Scer\UAS.18} in a Abl²/Abl⁴ background causes commisureless phenotypes in the ladder-like neuronal tracts in 19% of embryonic ventral ganglion segments.

(Yu et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Dscam1^RNAi.UAS.18 is partially rescued by Dscam1^{exon17.2.UAS.GFP}/Scer\GAL4^ey-OK107

(Shi et al., 2007)

Dscam1^RNAi.UAS.18 is partially rescued by Scer\GAL4^ey-OK107/Dscam1^{17.1-17.2.UAS.GFP}

(Shi et al., 2007)

Not rescued by

Dscam1^RNAi.UAS.18 is not rescued by Scer\GAL4^ey-OK107/Dscam1^{exon17.1.UAS.GFP}