The P{3'EPgy2} remnant (P{3'EPgy2}mthl1YO17) associated with the mthl1YO17 allele retains Scer\UAS regulatory sequences and a minimal promoter to direct expression of mthl1.
Imprecise excision of the progenitor insertion, resulting in a deletion which extends from 14F2, X:16522115 (release 5 coordinates) to the the P{EPgy2}mthl1EY16157 insertion site at 14F4, X:16535030 (release 5 coordinates). A portion of the original insertion, from nucleotide 6624 to the 3' P-element end remains associated with the deletion. The right breakpoint is within mthl1, generating the mthl1YO17 allele.
Df(1)YO17 heterozygous females containing Scer\GAL4mat.αTub67C.T:Hsim\VP16 crossed to wild type males produce progeny showing normal gastrulation. (Expression of mthl1 via the Scer\UAS sites remaining at the breakpoint of Df(1)YO17 (P{3'EPgy2}mthl1YO17, associated allele = mthl1YO17) rescue the gastrulation defects show by Df(1)YO17 embryos in the absence of a Scer\GAL4 driver.)
