The expression of TBPH^{dsRNA.UAS.cDa} under the control of Scer\GAL4^da.G32 induces significant locomotor defects in adults (progressive decrease in climbing behavior in negative geotaxis assays), but not in larvae.

Animals expressing TBPH^{dsRNA.Scer\UAS.cDa} under the control of either Scer\GAL4^Mef2.PR or Scer\GAL4^repo (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) eclose at the same frequency as controls. Performance of the mutant flies in a startle-induced negative geotaxis assay is the same as controls at both 7 and 14 days of age.

30 day old flies expressing TBPH^{dsRNA.Scer\UAS.cDa} under the control of either Scer\GAL4^Mef2.PR or Scer\GAL4^repo (in the presence of Dcr-2^Scer\UAS.cDa) show a significant reduction in overall motor activity, mean speed and total distance travelled compared to controls when motor behaviours are studied in an open-field paradigm.

Flies expressing TBPH^{dsRNA.Scer\UAS.cDa} under the control of Scer\GAL4^tub.PU exhibit severe gait abnormalities.

The electroretinograms from 1 and 5 day old flies expressing TBPH^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PU in response to blue light pulses are similar in peak-peak amplitude to controls. However these flies have a 25% reduction in the off-transient.

Pan-neuronal expression of TBPH^{dsRNA.Scer\UAS.cDa} (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^elav.PU results in impaired synaptic transmission at the third instar larval NMJ. The amplitude and frequency of miniature excitatory junction potential (mEJPs) are both reduced compared to controls. Low frequency (0.2Hz) stimulation of the NMJ shows no difference in evoked EJP amplitudes, resulting in an increased quantal content. No alterations to the mEJP are seen at the NMJ when TBPH^{dsRNA.Scer\UAS.cDa} is expressed in muscles under the control of Scer\GAL4^C57; both the amplitude and frequency are similar to controls. However, low level stimulation (0.2Hz) reveals a small but significant difference in evoked EJP amplitude, although this does not result in a significantly different quantal content.

Ubiquitous expression of TBPH^{dsRNA.Scer\UAS.cDa} (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^tub.PU causes larval/pupal lethality with few adult escapers, and a disturbed tripod gait. 5 day old adult flies display impaired motor behavior, with severely reduced walking activity and distance travelled. Their motor behavior remains limited over time with significantly reduced walking speed and walking is characterised by severe gait abnormalities. Similar motor defects are seen when TBPH^{dsRNA.Scer\UAS.cDa} is expressed in post-mitotic neurons under the control of Scer\GAL4^elav.PU, or in upper motor neurons under the control of Scer\GAL4^EB1.

Expression of TBPH^{dsRNA.Scer\UAS.cDa} in the upper motor neurons under the control of Scer\GAL4^EB1 does not significantly alter lifespan. The flies do not exhibit any differences at the synaptic termini of the EB ring neuropil at any of 5, 20 or 40 days after eclosion. However, by day 40 misshapen, fragmented and scattered cell bodies are observed, together with a severe reduction in the number and fasciculation of axonal projections when compared with age-matched controls. There are no significant differences in neuron numbers at days 5 and 20, but by day 40 and 50 a significant reduction in the number of targeted EB neurons is observed, and this phenotype worsens over time. The remaining neurons have misshapen, fragmented and scattered cell bodies when compared with age-matched controls.

Scer\GAL4^nSyb.PS, TBPH^RNAi.UAS.cDa has short lived phenotype, enhanceable by Npl4^KK102533, Scer\GAL4^nSyb.PS

Scer\GAL4^nSyb.PS, TBPH^RNAi.UAS.cDa has abnormal locomotor behavior | adult stage | progressive phenotype, enhanceable by Npl4^KK102533, Scer\GAL4^nSyb.PS

Scer\GAL4^nSyb.PS, TBPH^RNAi.UAS.cDa has abnormal locomotor behavior | adult stage | progressive phenotype, enhanceable by Scer\GAL4^nSyb.PS/Ufd1^KK108134

TBPH^RNAi.UAS.cDa, Scer\GAL4^nSyb.PS is an enhancer of short lived phenotype of Npl4^KK102533, Scer\GAL4^nSyb.PS

TBPH^RNAi.UAS.cDa, Scer\GAL4^nSyb.PS is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Npl4^KK102533, Scer\GAL4^nSyb.PS

Scer\GAL4^nSyb.PS/TBPH^RNAi.UAS.cDa is an enhancer of short lived phenotype of Scer\GAL4^nSyb.PS, Ufd1^KK108134

Scer\GAL4^nSyb.PS/TBPH^RNAi.UAS.cDa is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Scer\GAL4^nSyb.PS, Ufd1^KK108134