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General Information
Symbol
Dmel\domdsRNA.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0287784
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Two sets of UAS regulatory sequences drive expression in both directions of a 1057bp genomic dom fragment spanning the 3' end of exon 5, intron 5 and most of exon 6 (coordinates 2R:17215713..17216769 , release 5 genome), resulting in a dsRNA directed against dom.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 results in an incompletely penetrant phenotype of one or more nicks on the anterior wing margin, and increased cell death is observed in the wing disc, in a portion of the dorsal-ventral wing margin.

Expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4vg.PU results in wing nicking.

Expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4ptc.PU results in loss of the anterior crossvein, loss of head bristles and fusion of ocelli.

Expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4pnr-MD237 results in incomplete notum fusion.

Expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4dpp.blk1 results in loss of distal leg segments.

Expression of one copy of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 results in mild defects in the wing margin, mostly along the anterior margin. 57% of these flies show wing nicking. Expression of two copies of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 results in more widespread wing nicking.

84% of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 in a domk08108/+ background show wing nicking.

98% of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 in a dom3/+ background show wing nicking.

72% of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 in a dom9/+ background show wing nicking.

81% of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 in a domEP2371/+ background show wing nicking.

76% of flies co-expressing domJF01502 and domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 show wing nicking.

79% of flies co-expressing domdsRNA.Scer\UAS.cUa and domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 show wing nicking.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Scer\GAL4bbg-C96, domdsRNA.Sym.UAS has visible phenotype, enhanceable by DlBX9/Dl[+]

Scer\GAL4bbg-C96, domdsRNA.Sym.UAS has visible phenotype, enhanceable by mam1/mam[+]

NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4bbg-C96, domdsRNA.Sym.UAS has wing margin phenotype, enhanceable by mam1/mam[+]

NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Gcn5HMS00941, HP1cHMS00919, CG7745JF02132, jigr1JF02211, CG11723HMJ02079, CG3838JF02212, ERRJF02431, RpS9HMS00271, CG5181HMS00676, CG8289HMS00233, CG17385HMS00617, CG8924HMJ02055, AscizJF02336, Top1HMC04001, IswiHMS00628, Chd1HMS01142, Ino80HMS00586, XNPHMS00683, brmHMS00050, or Hel89BHMS00684, enhances the wing nicking phenotype of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96.

Co-expression of maf-SHMS02020, RYBPHMS00931, CG4617JF02015, ZifJF02524, Nup50HMS01054, Marcal1HMS00587, Etl1HMS00829, or kisHMS01254, suppresses the wing nicking phenotype of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96.

Co-expression of CG10949JF02129, CG15514JF02231, Dsp1JF02732, Chd3HMS00302, or okrHMS00585, does not enhance or suppress the wing nicking phenotype of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96.

Co-expression of Mi-2HMS00301 enhances the wing nicking phenotype of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96, and also leads to partial lethality.

Co-expression of Caf1-55HMS00051 or asf1HMC03937 enhances the wing nicking phenotype, and enhances the wing disc increased cell death along the dorsal-ventral wing margin of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96.

Co-expression of M1BPHMS02334 enhances the increased cell death along the dorsal-ventral wing margin of the wing disc seen in flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96.

Co-expression of HDAC1HMS00607 suppresses the wing nicking phenotype, and suppresses the wing disc increased cell death along the dorsal-ventral wing margin of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96.

Co-expression of PCNAHMS00634 enhances the wing nicking phenotype, but does not enhance the increased cell death in the wing disc of flies expressing domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also carrying one of DlBX9/+, Nnd-1/Y, sno71e1/Y or mam1/+, E(Pc)1, MRG15j6A3, Nipped-AKG10162 or reptEY12756.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also co-expressing Tip60HM05049.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is suppressed if the flies are also carrying taraEP-425, tara1 or Atg1EP-1202.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is suppressed if the flies are also co-expressing NICN.Scer\UAS.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is partially suppressed if the flies are also carrying Atg13, or are co-expressing wdbEP-1037 or wdbScer\UAS.cHa.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also carrying one of Pabp2EP-558, Pabp201, Lk6EP-573, Lk62, EcREP-939, EcRM554fs, lolaEP-1000, lolaORE119, lolaEP-1538, pum01688 or pumEP-1646.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also co-expressing either Tudor-SNEP-593, Tudor-SNEY07875, emcEP-1561, emcEY01657, lilliEP-1630 or lilliEY11976.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also co-expressing one of Atg1Scer\UAS.cTa, Atg6JF02897, Atg7JF02787, Atg8aJF02895, Atg8bJF02706, Atg12JF02704, Atg9JF02891, wdbHM05150, twsJF03141, PP2A-B'HM05256, mtsJF02805, Pp2A-29BJF03316, ordcoScer\UAS.cKa.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also carrying one wdb07.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is partially suppressed if the flies are also co-expressing dcoHM04075.

Co-expression of either Atg2JF02786, Atg18JF02898 or Atg4JF03003 has no effect on the wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96.

The wing nicking phenotype caused by expression of Rbf280.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also co-expressing domdsRNA.Sym.Scer\UAS.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Co-expression of domScer\UAS.B significantly rescued the wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 (penetrance is reduced from 57% to 20%).

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
domdsRNA.Sym.Scer\UAS
domdsRNA.Sym.UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)