FB2025_01 , released February 20, 2025
Allele: Dmel\Rcc1UASp.mCherry
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General Information
Symbol
Dmel\Rcc1UASp.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0287892
Feature type
allele
Associated gene
Dmel\Rcc1
Associated Insertion(s)
Carried in Construct
P{UASp-Rcc1.mCherry}
Also Known As
RCC1-mCherry
Key Links
Transgenic product class
wild_type
(Colombié et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Colombié et al., 2013)
Mutagen
in vitro construct
(Colombié et al., 2013)
Progenitor genotype
Carried in construct
P{UASp-Rcc1.mCherry}
Cytology
Description

UASp regulatory sequences drive expression of full length Rcc1 tagged with mCherry. A mutation (AAAATG to TAAATG) has been introduced upstream of the ATG to reduce the expression level.

(Colombié et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Rcc1
Tagged with
mCherry
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Rcc1Scer\UAS.P\T.T:Disc\RFP-mCherry under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in mild condensation defects - undercondensed chromosomes are found in only a fraction (3%) of mature oocytes.

      (Nikalayevich and Ohkura, 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The frequency of undercondensed chromosomes observed in mature oocytes expressing either Rcc1Scer\UAS.P\T.T:Disc\RFP-mCherry or Mi-2GL00318 RNAi under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is strongly increased by simultaneous expression of both of the transgenes, the undercondensed chromosomes are not only longer but also wider compared to controls and have higher median volume.

      (Nikalayevich and Ohkura, 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rcc1Scer\UAS.P\T.T:Disc\RFP-mCherry
      Rcc1UASp.mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (2)