FlyBase Allele Report: Dmel\step[RNAi.UAS]

General Information

Symbol

Dmel\step^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0287907

Feature type

allele

Associated gene

Dmel\step

Associated Insertion(s)

Carried in Construct

P{UAS-step-RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Hahn et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Hahn et al., 2013)

Mutagen

in vitro construct

(Hahn et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-step-RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 443bp step cDNA fragment (basepairs 589-1031 of step-RA cDNA, corresponding to the C-terminal pleckstrin homology domain) that forms a dsRNA fragment.

(Hahn et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

step

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

crossvein, with Df(2L)TW65, Scer\GAL4^en-e16E

(Hahn et al., 2013)

crossvein, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing, with Df(2L)TW65, Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing vein, with Df(2L)TW65, Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing vein, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing vein L4, with Df(2L)TW65, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Detailed Description

Statement

Reference

step^{dsRNA.Scer\UAS}-mediated knockdown of step in the posterior wing compartment, under the control of Scer\GAL4^en-e16E, results in a reduction of the wing size due to a reduction in cell number but not size. Furthermore, loss of wing vein tissue is observed and both cross veins are not formed properly. Reducing step levels further by RNAi mediated knockdown in a Df(2L)TW65 background enhances the wing vein phenotype with absent crossveins and gaps in the lateral vein L4.

(Hahn et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^en-e16E/step^RNAi.UAS is an enhancer of wing phenotype of Scer\GAL4^en-e16E, cnk^GD11960

(Hahn et al., 2013)

Scer\GAL4^en-e16E/step^RNAi.UAS is an enhancer of wing vein phenotype of Scer\GAL4^en-e16E, cnk^GD11960

(Hahn et al., 2013)

Scer\GAL4^en-e16E/step^RNAi.UAS is an enhancer of wing phenotype of Scer\GAL4^en-e16E, rl^GD4697

(Hahn et al., 2013)

Scer\GAL4^en-e16E/step^RNAi.UAS is an enhancer of wing vein phenotype of Scer\GAL4^en-e16E, rl^GD4697

(Hahn et al., 2013)

step^RNAi.UAS, Scer\GAL4^en-e16E is an enhancer of wing vein L4 phenotype of Egfr^KK100051, Scer\GAL4^en-e16E

(Hahn et al., 2013)

step^RNAi.UAS, Scer\GAL4^en-e16E is an enhancer of crossvein phenotype of Egfr^KK100051, Scer\GAL4^en-e16E

(Hahn et al., 2013)

step^RNAi.UAS, Scer\GAL4^en-e16E is an enhancer of wing phenotype of Egfr^KK100051, Scer\GAL4^en-e16E

(Hahn et al., 2013)

step^RNAi.UAS, Scer\GAL4^en-e16E is an enhancer of wing vein phenotype of Egfr^KK100051, Scer\GAL4^en-e16E

(Hahn et al., 2013)

step^RNAi.UAS, Scer\GAL4^en-e16E is an enhancer of wing phenotype of Ras85D^KK108029, Scer\GAL4^en-e16E

(Hahn et al., 2013)

step^RNAi.UAS, Scer\GAL4^en-e16E is an enhancer of wing vein phenotype of Ras85D^KK108029, Scer\GAL4^en-e16E

(Hahn et al., 2013)

step^RNAi.UAS, Scer\GAL4^en-e16E is an enhancer of wing phenotype of Raf^GD9831, Scer\GAL4^en-e16E

(Hahn et al., 2013)

step^RNAi.UAS, Scer\GAL4^en-e16E is an enhancer of wing vein phenotype of Raf^GD9831, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction of wing vein tissue seen upon expression of cnk^GD11960 in the posterior compartment of the imaginal wing disc under the control of Scer\GAL4^en-e16E can be further increased by co-expression of step^{dsRNA.Scer\UAS}.

Reducing Egfr levels by Egfr^KK100051-mediated knockdown in the posterior wing compartment (under the control of Scer\GAL4^en-e16E), in a step^{dsRNA.Scer\UAS} background generates a more severe Egfr phenotype. The lateral vein L4 is entirely lost, both cross veins are not differntiated and the distal end of the lateral vein L5 is missing.

Knockdown of Ras85D in a step^{dsRNA.Scer\UAS} background, through expression of Ras85D^KK108029 in the posterior wing compartment (both transgenes under the control of Scer\GAL4^en-e16E) generates a more severe Ras85D phenotype than in a wild-type background. The lateral vein L4 is entirely lost, both cross veins are not differentiated and the distal end of the lateral vein L5 is missing.

Knockdown of phl in a step^{dsRNA.Scer\UAS} background, through expression of phl^GD9831 in the posterior wing compartment (both transgenes under the control of Scer\GAL4^en-e16E) generates a more severe phl phenotype than in a wild-type background. The lateral vein L4 is entirely lost, both cross veins are not differntiated and the distal end of the lateral vein L5 is missing.

Knockdown of rl in a step^{dsRNA.Scer\UAS} background, through expression of rl^GD4697 in the posterior wing compartment (both transgenes under the control of Scer\GAL4^en-e16E) generates a more severe rl phenotype than in a wild-type background. The lateral vein L4 is entirely lost, both cross veins are not differntiated and the distal end of the lateral vein L5 is missing.

(Hahn et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

step^RNAi.UAS

step^{dsRNA.Scer\UAS}

step^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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