UAS regulates expression of wild type eya fused to a non-functional mutant nuclear localization sequence (Tag:NLS(SV40-largeT) mutated from PPKKKRKV to PPKAARKV).
eye | ectopic, with Scer\GAL4dpp.blk1
Scer\GAL4dpp.blk1 eyaWT.Scer\UAS.T:SV40\nls2-MUT flies exhibit an average of ~35% frequency of ectopic eye induction (compared to 49% frequency when eyaWT.Scer\UAS is expressed).
eyaWT.UAS.NLS-MUT/Scer\GAL4dpp.blk1 rescues eya2
Scer\GAL4dpp.blk1-mediated expression of eyaWT.Scer\UAS.T:SV40\nls2-MUT fully rescues the eyeless phenotype of eya2 animals.