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General Information
Symbol
Dmel\Zip89BUAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0288197
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dZip89Bflag, pUAST dZip89Bflag
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Zip89B which is tagged at the C-terminal end with Tag:FLAG.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4mex1.2.1 does not significantly affect survival rate as compared with wild type when flies are raised from first instar larval stage to adulthood on zinc-supplemented media (12mmol/L ZnCl[[2]]).

Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 results in a significant increase in survival as compared to wild type when flies are raised from first instar larval stage to adulthood on media supplemented with TPEN.

Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Zip89B.PR does not result in any obvious phenotypes or alter the survival of flies on any zinc-modulated diets.

Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long results in a mild rough eye phenotype, and expression of two copies of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long results in a severe rough eye phenotype.

Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4tub.PU results in delayed development.

Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PFa does not result in a mutant phenotype.

Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237 does not result in a mutant phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The adult eye phenotype seen upon expression of ZnT86DScer\UAS.cDa under the control of Scer\GAL4GMR.long is exacerbated by co-expression of Zip89BScer\UAS.T:Zzzz\FLAG : the morphological defects strongly so while the pigmentation loss is only mildly worsened. Co-expression of Zip89BScer\UAS.T:Zzzz\FLAG also leads to partial lethality.

The morphological and pigmentation defects in the adult eye seen upon expression of ZnT86DScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.long are strongly ameliorated by co-expression of Zip89BScer\UAS.T:Zzzz\FLAG.

The morphological and pigmentation defects in the adult eye seen upon expression of Zip71BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long are exacerbated by co-expression of Zip89BScer\UAS.T:Zzzz\FLAG.

Co-expression of foiScer\UAS.T:Zzzz\FLAG or ZnT41FVDRC.cUa moderately exacerbates the rough eye phenotype of flies expressing Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long.

Co-expression of Zip42C.1Scer\UAS.T:Zzzz\FLAG, Zip42C.1Scer\UAS.T:Avic\GFP-EGFP, Zip42C.2Scer\UAS.T:Zzzz\FLAG or ZnT63CVDRC.cUa severely exacerbates the rough eye phenotype of flies expressing Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long.

Co-expression of Zip89BScer\UAS.T:Zzzz\FLAG partially rescues eye phenotypes of flies expressing ZnT86DScer\UAS.T:Disc\RFP-mCherry or ZnT86DScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.long.

Co-expression of Zip89BScer\UAS.T:Zzzz\FLAG and Zip42C.1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PFa results in a flattened eye.

Co-expression of Zip89BScer\UAS.T:Zzzz\FLAG and Zip42C.1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4pnr-MD237 results in an abnormal thoracic bristle alignment.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4mex1.2.1 fully restores survival of Zip89BΔ/Zip89BΔ flies on high-zinc media back to wild type levels.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Zip89BScer\UAS.T:Zzzz\FLAG
Zip89BUAS.Tag:FLAG
dZip89Bflag
Name Synonyms
Secondary FlyBase IDs
    References (4)