UAS regulatory sequences drive expression of plum lacking the Ig4 domain (amino acid residues 356-451 have been deleted). The protein is tagged with Tag:FLAG.
abnormal neuroanatomy | adult stage, with Scer\GAL4ey-OK107
abnormal size | adult stage, with Scer\GAL4ey-OK107
adult mushroom body beta-lobe, with Scer\GAL4ey-OK107
Expressing plumΔIg4.UAS.Tag:FLAG under the control of Scer\GAL4ey-OK107 results occasionally in adult mushroom body β-lobe retraction.
plumΔIg4.UAS.Tag:FLAG/Scer\GAL4Tab2-201Y fails to rescue plumΔ1
plumΔIg4.UAS.Tag:FLAG/Scer\GAL4elav.PU fails to rescue plumΔ2
Expression of plumΔIg4.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Tab2-201Y shows very little ability to rescue the γ neuron pruning defects seen in plumΔ1 mushroom body neuroblast clones (rescue seen in 1 out of 16 cases).
Expression of plumΔIg4.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav.PU fails to rescue the formation of ectopic connections at the neuromuscular junction in plumΔ2 larvae.