A 891 base pair fragment from the flanking non-coding or intronic region of Lgr3 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Ecol\lexAGMR17G11 drives expression in several cells in the male adult abdominal neuromere that Scer\GAL4VP16.Lgr3 also drives expression in, but not the female. It also drives expression in a non dimorphic cells in the brain.
Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.