FB2020_06, released Dec 22, 2020

Allele: Ecol\lexAGMR19B09

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General Information
Symbol
Ecol\lexAGMR19B09
Species
E. coli
Name
FlyBase ID
FBal0289026
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
Carried in Construct
P{GMR19B09-lexA}
Also Known As
R19B09-LexA
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Pfeiffer et al., 2013.10.9)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{GMR19B09-lexA}
      Cytology
      Nature of the lesion
      Statement
      Reference

      A 3683 base pair fragment from the flanking non-coding or intronic region of Lgr3 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

      (Pfeiffer et al., 2013.10.9)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      lexA::p65
      Associated Sequence Features
      GMR19B09
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      late third instar larval stage
      embryonic/larval neuron | subset of embryonic/larval central nervous system
      (Colombani et al., 2015)
      larval insulin secreting cell of pars intercerebralis
      (Colombani et al., 2015)
      larval stage
      embryonic/larval central nervous system | restricted
      (Vallejo et al., 2015)
      larval insulin secreting cell of pars intercerebralis
      (Vallejo et al., 2015)
      adult stage | female
      adult median bundle | intense
      (Meissner et al., 2016)
      adult stage | male
      adult median bundle | faint
      (Meissner et al., 2016)
      third instar larval stage
      larval pars intercerebralis | restricted
      (Garelli et al., 2015)
      Additional Information
      Statement
      Reference

      Ecol\lexAGMR19B09 colocalizes with Scer\GAL4VP16.Lgr3 in the median bundle of females, and has lower expression in males.

      (Meissner et al., 2016)

      GFP reconstitution across synaptic partners (GRASP) shows synaptic connections between Scer\GAL4Ptth.cMa-positive prothoracic gland innervating neurons and Ecol\lexAGMR19B09-positive dorsomedial neurosecretory cells.

      (Colombani et al., 2015)

      Ecol\lexAGMR19B09 drives expression in around 12 neurons per hemisphere in the central larval brain as well as some neurons in the ventral nerve cord. GFP reconstitution across synaptic partners (GRASP) shows extensive connections to Scer\GAL4Ilp3.PC positive neurons.

      (Vallejo et al., 2015)

      Ecol\lexAGMR19B09 co-expresses with Scer\GAL4Mz699 in a bilateral pair of neurons in the larval pars intercerebralis.

      (Garelli et al., 2015)
       
      Marker for
      Reflects expression of
      Lgr3
      (Meissner et al., 2016, Vallejo et al., 2015)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.

      (Pfeiffer et al., 2013.10.9)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ecol\lexAGMR19B09
      Name Synonyms
      Secondary FlyBase IDs
        References (5)