FB2025_01 , released February 20, 2025
Allele: Ecol\lexAGMR58E02
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General Information
Symbol
Ecol\lexAGMR58E02
Species
E. coli
Name
FlyBase ID
FBal0289823
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
Carried in Construct
P{GMR58E02-lexA}
Also Known As
R58E02-LexA, 58E02-LexA, 58E02-LexAp65
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2013.10.9)
Progenitor genotype
Carried in construct
P{GMR58E02-lexA}
Cytology
Description

A 1235 base pair fragment from the flanking non-coding or intronic region of DAT is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR58E02
Encoded product / tool
lexA::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval mushroom body dopaminergic neuron h1
      (Eschbach et al., 2020)
      larval mushroom body dopaminergic neuron j1
      (Eschbach et al., 2020)
      larval mushroom body dopaminergic neuron i1
      (Eschbach et al., 2020)
      adult stage
      adult dopaminergic PAM neuron
      (Dag et al., 2019, Owald et al., 2015, Yamagata et al., 2015, Liu et al., 2012)
      adult optic lobe glial cell
      (Liu et al., 2012)
      adult brain | restricted
      (Yamagata et al., 2015)
      dopaminergic PAM neuron 1
      (Dag et al., 2019)
      adult dopaminergic PAM neuron | subset
      (Lei et al., 2022)
      adult neuron | subset of adult brain
      (Lei et al., 2022)
      alpha/beta core Kenyon cell
      (Ahmed et al., 2023)
      Additional Information
      Statement
      Reference

      Drives expression in dopaminergic PAM neurons and larval dopaminergic pPAM neurons.

      (FlyBase Curators, 2017)

      EcollexAGMR58E02 positive dopaminergic PAM neurons are in close proximity with ScerGAL4GMR66C08 expressing mushroom body medial lobe arborizing neuron 6 as shown by a positive GFP reconstitution across synaptic partners (GRASP) signal.

      (Owald et al., 2015)

      Some dopaminergic PAM neurons labelled by EcollexAGMR58E02 are also co-labelled by ScerGAL4ple.PF.

      (Liu et al., 2012)
       
      Marker for
      Reflects expression of
      DAT
      (Liu et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA113xlexAop.JFRC26 at 30[o]C) of Ecol\lexAGMR58E02 cells in starved adults, in combination with a paired odor, results in a significant increase in 2-min associative attractive olfactory memory, compared to controls.

      (Liu et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Ecol\lexAGMR58E02, TrpA113xlexAop.JFRC26 has abnormal memory | adult stage | heat sensitive phenotype, non-enhanceable by Scer\GAL4Mef2.247/Dop1R1f02676

      (Liu et al., 2012)
      Suppressed by
      Statement
      Reference

      Ecol\lexAGMR58E02, TrpA113xlexAop.JFRC26 has abnormal memory | adult stage | heat sensitive phenotype, suppressible by Dop1R1f02676

      (Liu et al., 2012)
      NOT suppressed by
      Statement
      Reference

      Ecol\lexAGMR58E02, TrpA113xlexAop.JFRC26 has abnormal memory | adult stage | heat sensitive phenotype, non-suppressible by Scer\GAL4Mef2.247/Dop1R1f02676

      (Liu et al., 2012)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.

      (Pfeiffer et al., 2013.10.9)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ecol\lexAGMR58E02
      Name Synonyms
      Secondary FlyBase IDs
        References (28)