FlyBase Allele Report: Dmel\tutl[RE40452.UAS]

General Information

Symbol

Dmel\tutl^RE40452.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0290261

Feature type

allele

Associated gene

Dmel\tutl

Associated Insertion(s)

Carried in Construct

P{UAS-tutl.RE40452}

Also Known As

UAS-tutl

Key Links

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Ferguson et al., 2009, Long et al., 2009)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-tutl.RE40452}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of tutl full-length coding sequences.

(Ferguson et al., 2009)

UASt regulatory sequences drive expression of a 5.2kb EcoRI/BamHI fragment from the tutl RE40452 cDNA.

(Long et al., 2009)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tutl

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

(Long et al., 2009)

Phenotype Manifest In

dendrite & dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Long et al., 2009)

Detailed Description

Statement

Reference

Expression of tutl^Scer\UAS.cLa under the control of Scer\GAL4^ppk.1.9 reduces dendritic branching in ddaC neurons.

(Long et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

bdl^EX2, tutl^RE40452.UAS, Scer\GAL4^GMR.long is a non-suppressor of abnormal neuroanatomy phenotype of tutl²³

(Cameron et al., 2013)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

bdl^EX2, tutl^RE40452.UAS, Scer\GAL4^GMR.long is a non-suppressor of rhabdomere R7 phenotype of tutl²³

(Cameron et al., 2013)

bdl^EX2, tutl^RE40452.UAS, Scer\GAL4^GMR.long is a non-suppressor of axon phenotype of tutl²³

(Cameron et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Fails to rescue

Scer\GAL4^VGlut-OK371/tutl^RE40452.UAS fails to rescue tutl²³

(Zhou et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^Ddc.PL fails to rescue tutl²³

(Zhou et al., 2012)

Scer\GAL4^eve.RN2/tutl^RE40452.UAS fails to rescue tutl²³

(Zhou et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^G11-1 fails to rescue tutl²³

(Zhou et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^ftz.ng fails to rescue tutl²³

(Zhou et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^Toll-6-D42 fails to rescue tutl²³

(Zhou et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^TrpA1.PR fails to rescue tutl²³

(Zhou et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^5-HTR1B fails to rescue tutl²³

(Zhou et al., 2012)

Scer\GAL4^c81/tutl^RE40452.UAS fails to rescue tutl²³

(Zhou et al., 2012)

Scer\GAL4^5-40/tutl^RE40452.UAS fails to rescue tutl²³

(Zhou et al., 2012)

Scer\GAL4^nompC.PC/tutl^RE40452.UAS fails to rescue tutl²³

(Zhou et al., 2012)

Scer\GAL4^iav.PK/tutl^RE40452.UAS fails to rescue tutl²³

(Zhou et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^pain-GAL4 fails to rescue tutl²³

(Zhou et al., 2012)

tutl^RE40452.UAS/Scer\GAL4^ppk.1.9 fails to rescue tutl²³

(Zhou et al., 2012)

Comments

The defect in navigational response to gentle touch which is seen in homozygous tutl²³ larvae is rescued by expression of tutl^{RE40452.Scer\UAS} under the control of one of Scer\GAL4^elav-C155, Scer\GAL4^Appl.G1a or Scer\GAL4^Cha.7.4. Expression under the control of one of Scer\GAL4^VGlut-OK371, Scer\GAL4^Ddc.PL, Scer\GAL4^eve.RN2, Scer\GAL4^G11-1, Scer\GAL4^ftz.ng, Scer\GAL4^D42, Scer\GAL4^TrpA1.PR, Scer\GAL4^5-HTR1B, Scer\GAL4^c81, Scer\GAL4^5-40, Scer\GAL4^nompC.PC, Scer\GAL4^iav.PK, Scer\GAL4^pain-GAL4 or Scer\GAL4^ppk.1.9 does not rescue the mutant phenotype.

Expression of tutl^{RE40452.Scer\UAS} under the control of Scer\GAL4^elav-C155 from the beginning of the larval stage onwards is sufficient to rescue the defect in navigational response to gentle touch seen in homozygous tutl²³ larvae, while limiting expression to the embryonic stage results in a failure of rescue (the temperature-sensitive Scer\GAL80^ts.αTub84B allele has been used to limit the stage of expression).

(Zhou et al., 2012)

Expression of tutl^Scer\UAS.cFa under the control of Scer\GAL4^GMR.long rescues the mild tiling phenotype found in tutl^k14703/tutl²³ R7 terminals.

(Ferguson et al., 2009)

Expression of tutl^Scer\UAS.cLa under the control of Scer\GAL4²²¹ restores the number of dendrite branch termini per cell to wild-type levels in tutl⁰¹⁰⁸⁵/tutl²³ ddaE neurons.

Expression of tutl^Scer\UAS.cLa under the control of Scer\GAL4^elav-C155 rescues the viability of tutl⁰¹⁰⁸⁵/tutl²³ mutants to 93%.

(Long et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

tutl^{RE40452.Scer\UAS}

tutl^RE40452.UAS

tutl^Scer\UAS.cFa

tutl^Scer\UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Long

Secondary FlyBase IDs

FBal0241229
FBal0243340

References (4)

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