FB2025_01 , released February 20, 2025
Allele: Dmel\mir-9cKO
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General Information
Symbol
Dmel\mir-9cKO
Species
D. melanogaster
Name
FlyBase ID
FBal0290289
Feature type
allele
Associated gene
Dmel\mir-9c
Associated Insertion(s)
TI{TI}mir-9cKO
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)
Mutagen
ends-out gene targeting
(Chen et al., 2014, Kugler et al., 2013)
cre recombinase
(Chen et al., 2014, Kugler et al., 2013)
FLPase
(Chen et al., 2014)
I-SceI
(Chen et al., 2014)
Progenitor genotype
Associated Insertion(s)
TI{TI}mir-9cKO
Cytology
Description

The mir-9c hairpin has been deleted. A single loxP site is present at the site of the deletion. It is not known whether or not expression of the host grp gene is affected.

(Chen et al., 2014)

Deletion mutation. A single loxP site is also present.

(Kugler et al., 2013)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos derived from mir-9cKO/Df(2L)Exel6039 embryos show a significant decrease in the number of primordial germ cells compared to embryos from control females.

      mir-9cKO/Df(2L)Exel6039 embryos show a significant decrease in primordial germ cell number compared to controls.

      Homozygous adults show significant defects in the hemolymph-brain barrier (as determined using a dye injection assay).

      (Chen et al., 2014)

      Stage 10 embryos of mir-9cKO mutant mothers have on average 20% fewer primordial germ cells (PGCs) than control embryos. In addition, the range of PGC numbers is broader in embryos from miRNA mutant mothers than from controls.

      (Kugler et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      mir-9cKO is rescued by mir-9cUAS.cluster.cSa

      (Kugler et al., 2013)
      Comments

      Maternal expression of Scer\GAL4nos.UTR.T:Hsim\VP16>mir-9cScer\UAS.cluster.cSa rescues the primordial germ cell phenotype of mir-9cKO mutants.

      (Kugler et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      miR-9cKO
      (Kugler et al., 2013)
      mir-9cKO
      (Niu et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)