FB2025_01 , released February 20, 2025
Allele: Dmel\staiKO
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General Information
Symbol
Dmel\staiKO
Species
D. melanogaster
Name
FlyBase ID
FBal0290338
Feature type
allele
Associated gene
Dmel\stai
Associated Insertion(s)
TI{TI}staiKO
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ends-out gene targeting
(Yang et al., 2012)
Progenitor genotype
Associated Insertion(s)
TI{TI}staiKO
Cytology
Description

Part of the stai gene (sequence starting at exon 6 and finishing at exon 8) has been replaced by a w+mW.hs marker. This results in the disruption of translation from Thr55 (coordinates as in the stai A isoform).

(Yang et al., 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  autosomal recessive intellectual developmental disorder
      modeled by Nab2EP3716
      (Rounds et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      A significant number of egg chambers show delays in border cell migration in homozygous females. Initiation of border cell migration is normal, however, once migration has started there is a significant reduction in net migration speed compared to wild type. The mutant migrating border cells show an abnormal distribution of cell extensions: there is a significant increase in the number of extensions that extend from the back and side of the cluster (where front is towards the oocyte) compared to controls.

      A significant number of egg chambers show delays in border cell migration in females containing homozygous border cell clones. Initiation of border cell migration is inefficient compared to controls, but once migration has started, net migration speed is normal. The distribution of cell extensions in the mutant migrating border cells is not significantly different from wild type. The migrating cluster often retains an attachment to the anterior end of the egg chamber for a long period, indicating that one border cell is still attached to a non-border cell from the epithelium. These "attached" clusters migrate more slowly than those without an attachment.

      (Yang et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      stai[+]/staiKO is a suppressor | partially of visible | adult stage phenotype of Nab2EP3716, Scer\GAL4GMR.PU

      (Rounds et al., 2022)

      stai[+]/staiKO is a suppressor of abnormal eye color phenotype of Nab2EP3716, Scer\GAL4GMR.PU

      (Rounds et al., 2022)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      stai[+]/staiKO is a suppressor | partially of eye phenotype of Nab2EP3716, Scer\GAL4GMR.PU

      (Rounds et al., 2022)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      staiKO is rescued by staitub.A

      (Yang et al., 2012)

      staiKO is rescued by staiUAS.A/Scer\GAL4slbo.2.6

      (Yang et al., 2012)
      Comments

      staitub.A rescues the reduced net cluster speed seen in migrating border follicle cells in homozygous staiKO females.

      Expression of staiScer\UAS.A under the control of Scer\GAL4slbo.2.6 rescues the inefficient initiation of border follicle cell migration seen in females containing homozygous border cell clones.

      (Yang et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      staiKO
      (Rounds et al., 2022, Bu et al., 2021, Hahn et al., 2021, Yang et al., 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)