FlyBase Allele Report: Dmel\bsk[DN.UAS.cUa]

General Information

Symbol

Dmel\bsk^DN.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0290703

Feature type

allele

Associated gene

Dmel\bsk

Associated Insertion(s)

Carried in Construct

P{UAS-bsk.DN.U}

Also Known As

UAS-bsk^DN, bsk^DN, UAS-Bsk-DN, UAS-Basket^DN, bskDN, Bsk-DN, UAS-JNK DN

Key Links

Transgenic product class

dominant_negative_variant

(Griswold et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Griswold et al., 2008)

Mutagen

in vitro construct

(Griswold et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-bsk.DN.U}

Cytology

Description

UAS regulatory sequences drive expression of a dominant-negative form of bsk.

(Griswold et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

bsk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates rhabdoid cancer

modeled by Snr1^HMS00363, BacA\p35^UAS.cHa

(Xie et al., 2017)

ameliorates cancer

modeled by Bub3^GD9924

(Barrio et al., 2023)

ameliorates colorectal cancer

modeled by Uvrag^HMS01357

(Nagy et al., 2016)

ameliorates carcinoma

modeled by Mef2^UAS.cUa, N^ICN.UAS

(Pallavi et al., 2012)

modeled by Ras85D^V12.UAS, msn³²⁰⁸

(Kong et al., 2021)

exacerbates carcinoma

modeled by Vps25^N55

(Woodfield et al., 2013)

modeled by scrib¹

(Kong et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Tab2-201Y, Scer\GAL4^UAS.cHa

(Bornstein et al., 2015)

abnormal size | larval stage, with Scer\GAL4^A58

(Lee et al., 2019)

abnormal wound healing | larval stage, with Scer\GAL4²²¹

(Rao et al., 2016)

abnormal wound healing | larval stage, with Scer\GAL4^A58

(Lee et al., 2019)

decreased cell size | larval stage | conditional, with Scer\GAL4^A58

(Lee et al., 2019)

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

decreased size | adult stage, with Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

visible | adult stage, with Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

axon, with Scer\GAL4^Tab2-201Y, Scer\GAL4^UAS.cHa

(Bornstein et al., 2015)

dendrite | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

embryonic/larval epidermis | larval stage, with Scer\GAL4^A58

(Lee et al., 2019)

embryonic/larval epidermis | larval stage | conditional, with Scer\GAL4^A58

(Lee et al., 2019)

intestinal stem cell | chemical conditional, with Scer\GAL4^NP0001

(Tang et al., 2013)

intestinal stem cell | chemical conditional, with Scer\GAL4^NP5130

(Tang et al., 2013)

larval dorsal multidendritic neuron ddaE | larval stage, with Scer\GAL4²²¹

(Rao et al., 2016)

larval dorsal multidendritic neuron ddaE | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

microtubule | larval stage, with Scer\GAL4²²¹

(Rao et al., 2016)

microtubule | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

nucleus | larval stage, with Scer\GAL4^A58

(Lee et al., 2019)

wing, with Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

Detailed Description

Statement

Reference

The expression of bsk^DN.UAS.cUa under the control of Scer\GAL4^ptc.PU induces a small wing.

(Ahmad et al., 2021)

The epidermis of larvae expressing bsk^DN.UAS.cUa under the control of Scer\GAL4^A58 shows fully prevalent wound closure defects.

(Lee et al., 2019)

The adulthood-only expression of bsk^DN.UAS.cUa under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) does not significantly affect the number of stem cells/enteroblasts in the adult midgut, despite decreasing the mitotic index of this epithelium, as compared to controls.

(Nagy et al., 2016)

The expression of bsk^{DN.Scer\UAS.cUa} under the control of Scer\GAL4²²¹ in ddaE neurons leads a defective early response to injury, as the pool of growing microtubules (identified as fluorescent EB1 comets) fails to show the expected reversal of polarity at 24h after laser-induced severance, as compared to controls.

(Rao et al., 2016)

Overexpression of bsk^{DN.Scer\UAS.cUa} in combination with Scer\GAL4^Scer\UAS.cHa (but not expression of bsk^{DN.Scer\UAS.cUa} alone) driven by Scer\GAL4^Tab2-201Y results in axon (but not dendrite) pruning defects.

(Bornstein et al., 2015)

ddaC neurons show robust dendrite regeneration after dendrite removal in animals expressing bsk^{DN.Scer\UAS.cUa} under the control of Scer\GAL4⁴⁷⁷.

ddaE neurons show robust dendrite regeneration after dendrite removal in animals expressing bsk^{DN.Scer\UAS.cUa} under the control of Scer\GAL4²²¹.

(Stone et al., 2014)

Expression of bsk^{DN.Scer\UAS.cUa} under the control of either Scer\GAL4^esg-NP5130 or Scer\GAL4^{Myo31DF-NP0001} results in impaired intestinal stem cell proliferation in response to paraquat.

(Tang et al., 2013)

Larval ddaE neurons expressing bsk^DN.UAS.cUa under the control of Scer\GAL4²²¹ (and Dicer-2, for a more efficient RNAi) show decreased growing microtubules within dendrites 8h after axotomy and show a decrease in the proportion of dendrites persisting after consecutive axotomy and dendrite severance (i.e. axotomy-8h-dendrite severance-18h), as compared to similarly injured control neurons; the former does not occur upon a 24h interval between axotomoy and dendrite severance.

(Chen et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc.PU, bsk^DN.UAS.cUa has visible | adult stage phenotype, non-enhanceable by syd^GLC01419/Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

Scer\GAL4^ptc.PU, bsk^DN.UAS.cUa has decreased size | adult stage phenotype, non-enhanceable by syd^GLC01419/Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^ptc.PU, bsk^DN.UAS.cUa has visible | adult stage phenotype, suppressible by syd^UAS.GFP/Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

Scer\GAL4^ptc.PU, bsk^DN.UAS.cUa has decreased size | adult stage phenotype, suppressible by syd^UAS.GFP/Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, bsk^DN.UAS.cUa, msn³²⁰⁸ has neoplasia | larval stage | somatic clone - tissue specific phenotype, suppressible by wts^UAS.cUa, Scer\GAL4^Act5C.PI

(Kong et al., 2021)

Scer\GAL4^Act5C.PI, bsk^DN.UAS.cUa, scrib¹ has increased size | larval stage | somatic clone - tissue specific phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/msn^UAS.cSa

(Kong et al., 2021)

Enhancer of

Statement

Reference

bsk^DN.UAS.cUa is an enhancer of abnormal neuroanatomy phenotype of Fas2^UAS.cLa, Scer\GAL4^Tab2-201Y

(Bornstein et al., 2015)

NOT Enhancer of

Statement

Reference

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^ptc.PU, syd^GLC01419

(Ahmad et al., 2021)

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a non-enhancer of decreased size | adult stage phenotype of Scer\GAL4^ptc.PU, syd^GLC01419

(Ahmad et al., 2021)

Suppressor of

Statement

Reference

bsk^DN.UAS.cUa, Scer\GAL4^en.PU is a suppressor of increased cell death | larval stage phenotype of Nkap^GD11807, Scer\GAL4^en.PU

(Guo et al., 2024)

bsk^DN.UAS.cUa/Scer\GAL4^esg.PU is a suppressor of hyperplasia | adult stage phenotype of Scer\GAL4^esg.PU, synr^{UAS.ORF.GW.Tag:HA}

(Sulekh et al., 2024)

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a suppressor | partially of increased cell death | larval stage phenotype of Scer\GAL4^ptc.PU, spoon^UAS.Tag:HA

(Das et al., 2023)

bsk^DN.UAS.cUa, Scer\GAL4^Act5C.PI is a suppressor | partially of neoplasia | larval stage | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, msn³²⁰⁸

(Kong et al., 2021)

bsk^DN.UAS.cUa, Scer\GAL4^Act5C.PI is a suppressor of some die during larval stage | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, emei¹

(Ma et al., 2020)

bsk^DN.UAS.cUa, Scer\GAL4^Act5C.PI is a suppressor of increased occurrence of cell division | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, emei¹

(Ma et al., 2020)

bsk^DN.UAS.cUa, Scer\GAL4^Act5C.PI is a suppressor of neoplasia | somatic clone | larval stage phenotype of Orai^UAS.cZa, Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Stim^UAS.cEa

(Ma et al., 2020)

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a suppressor of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^ptc.PU, lic^UAS.ORF

(Sun et al., 2019)

bsk^DN.UAS.cUa, Scer\GAL4^ptc.PU is a suppressor of abnormal cell migration | third instar larval stage phenotype of Hsap\MAP2K3^UAS.cSa, Scer\GAL4^ptc.PU

(Sun et al., 2019)

bsk^DN.UAS.cUa/Scer\GAL4^Tub.PU is a suppressor | somatic clone of abnormal cell migration | somatic clone phenotype of Pi3K59F^Δm22

(O'Farrell et al., 2017)

bsk^DN.UAS.cUa, Scer\GAL4^Act.PU is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363

(Xie et al., 2017)

bsk^DN.UAS.cUa/Scer\GAL4^NP5130 is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

bsk^DN.UAS.cUa/Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

bsk^DN.UAS.cUa, Scer\GAL4^Act.PU is a suppressor | partially | somatic clone of neoplasia | somatic clone phenotype of Ets21C^KK103211, Ras85D^V12.UAS, Scer\GAL4^Act.PU

(Külshammer et al., 2015)

bsk^DN.UAS.cUa, Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Atg9^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Tang et al., 2013)

bsk^DN.UAS.cUa, Scer\GAL4^GMR.PU is a suppressor of increased cell death | third instar larval stage phenotype of Atg9^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Tang et al., 2013)

Scer\GAL4^Act5C.PU/bsk^DN.UAS.cUa is a suppressor of increased cell death | embryonic stage phenotype of Scer\GAL4^Act5C.PU, dpp^UAS.cUa

(Yang and Su, 2011)

Scer\GAL4^how-24B/bsk^DN.UAS.cUa is a suppressor of increased cell death | embryonic stage phenotype of Scer\GAL4^how-24B, Tak1^UAS.cTa

(Yang and Su, 2011)

bsk^DN.UAS.cUa/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

bsk^DN.UAS.cUa/Scer\GAL4^GMR.PU is a suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

NOT Suppressor of

Statement

Reference

bsk^DN.UAS.cUa, Scer\GAL4^Bx-MS1096 is a non-suppressor of increased size | larval stage phenotype of Nkap^GD11807, Scer\GAL4^Bx-MS1096

(Guo et al., 2024)

bsk^DN.UAS.cUa, Scer\GAL4^Act.PU is a non-suppressor | somatic clone of abnormal developmental rate | somatic clone phenotype of Ets21C^KK103211, Ras85D^V12.UAS, Scer\GAL4^Act.PU

(Külshammer et al., 2015)

Scer\GAL4^en.PU/bsk^DN.UAS.cUa is a non-suppressor of increased cell death | third instar larval stage phenotype of Ipk2^20B

(Seeds et al., 2015)

Scer\GAL4^ap-md544/bsk^DN.UAS.cUa is a non-suppressor of visible phenotype of Scer\GAL4^ap-md544, Tap42^GD8900

(Wang et al., 2012)

Other

Statement

Reference

Scer\GAL4^Act5C.PI, bsk^DN.UAS.cUa, scrib¹ has increased size | larval stage | somatic clone - tissue specific phenotype

(Kong et al., 2021)

Hsap\NCAM1^140.UAS, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has abnormal neuroanatomy phenotype

(Bornstein et al., 2015)

Fas2^{UAS.ΔCT.KNSAV.Tag:FLAG}, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has abnormal neuroanatomy phenotype

(Bornstein et al., 2015)

Fas1^+3.UAS, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has abnormal neuroanatomy phenotype

(Bornstein et al., 2015)

Nrg^180.UAS, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has abnormal neuroanatomy phenotype

(Bornstein et al., 2015)

Fas2^UAS.cLb, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has abnormal neuroanatomy phenotype

(Bornstein et al., 2015)

Fas2^{UAS.PEST+.KNSAV.Tag:FLAG}, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has abnormal neuroanatomy phenotype

(Bornstein et al., 2015)

Fas2^{UAS.PEST-.KNSAV.Tag:FLAG}, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has abnormal neuroanatomy phenotype

(Bornstein et al., 2015)

Fas2^{UAS.CTshort.KNSAV.Tag:FLAG}, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has abnormal neuroanatomy phenotype

(Bornstein et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc.PU, bsk^DN.UAS.cUa has wing phenotype, non-enhanceable by syd^GLC01419/Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^ptc.PU, bsk^DN.UAS.cUa has wing phenotype, suppressible by syd^UAS.GFP/Scer\GAL4^ptc.PU

(Ahmad et al., 2021)

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, bsk^DN.UAS.cUa, msn³²⁰⁸ has eye disc | somatic clone - tissue specific phenotype, suppressible by wts^UAS.cUa, Scer\GAL4^Act5C.PI

(Kong et al., 2021)

Scer\GAL4^Act5C.PI, bsk^DN.UAS.cUa, scrib¹ has eye disc | somatic clone - tissue specific phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/msn^UAS.cSa

(Kong et al., 2021)

Enhancer of

Statement

Reference

bsk^DN.UAS.cUa is an enhancer of axon phenotype of Fas2^UAS.cLa, Scer\GAL4^Tab2-201Y

(Bornstein et al., 2015)

NOT Enhancer of

Statement

Reference

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a non-enhancer of wing phenotype of Scer\GAL4^ptc.PU, syd^GLC01419

(Ahmad et al., 2021)

Suppressor of

Statement

Reference

bsk^DN.UAS.cUa, Scer\GAL4^en.PU is a suppressor of wing disc | larval stage phenotype of Nkap^GD11807, Scer\GAL4^en.PU

(Guo et al., 2024)

bsk^DN.UAS.cUa/Scer\GAL4^esg.PU is a suppressor of adult gut phenotype of Scer\GAL4^esg.PU, synr^{UAS.ORF.GW.Tag:HA}

(Sulekh et al., 2024)

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a suppressor | partially of anterior crossvein phenotype of Scer\GAL4^ptc.PU, spoon^UAS.Tag:HA

(Das et al., 2023)

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a suppressor | partially of wing blade phenotype of Scer\GAL4^ptc.PU, spoon^UAS.Tag:HA

(Das et al., 2023)

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a suppressor | partially of wing disc phenotype of Scer\GAL4^ptc.PU, spoon^UAS.Tag:HA

(Das et al., 2023)

bsk^DN.UAS.cUa, Scer\GAL4^Act5C.PI is a suppressor | partially of eye disc | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, msn³²⁰⁸

(Kong et al., 2021)

bsk^DN.UAS.cUa, Scer\GAL4^Act5C.PI is a suppressor of eye disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, emei¹

(Ma et al., 2020)

bsk^DN.UAS.cUa, Scer\GAL4^Act5C.PI is a suppressor of eye disc | somatic clone phenotype of Orai^UAS.cZa, Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, Stim^UAS.cEa

(Ma et al., 2020)

bsk^DN.UAS.cUa/Scer\GAL4^ptc.PU is a suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc.PU, lic^UAS.ORF

(Sun et al., 2019)

bsk^DN.UAS.cUa, Scer\GAL4^ptc.PU is a suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Hsap\MAP2K3^UAS.cSa, Scer\GAL4^ptc.PU

(Sun et al., 2019)

(Katheder et al., 2017)

(Katheder et al., 2017)

bsk^DN.UAS.cUa, Scer\GAL4^{Act5C.CoinFLP-FRT} is a suppressor | cell non-autonomous | somatic clone of autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689

(Katheder et al., 2017)

bsk^DN.UAS.cUa, Scer\GAL4^{Act5C.CoinFLP-FRT} is a suppressor | cell non-autonomous | somatic clone of eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689

(Katheder et al., 2017)

bsk^DN.UAS.cUa, Scer\GAL4^Act.PU is a suppressor of autophagosome | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Katheder et al., 2017)

bsk^DN.UAS.cUa, Scer\GAL4^Act.PU is a suppressor of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib¹

(Katheder et al., 2017)

bsk^DN.UAS.cUa/Scer\GAL4^Tub.PU is a suppressor | somatic clone of follicle cell | somatic clone phenotype of Pi3K59F^Δm22

(O'Farrell et al., 2017)

bsk^DN.UAS.cUa, Scer\GAL4^Act.PU is a suppressor of wing disc | somatic clone | third instar larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363

(Xie et al., 2017)

bsk^DN.UAS.cUa/Scer\GAL4^NP5130 is a suppressor of adult midgut epithelium phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

bsk^DN.UAS.cUa/Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

bsk^DN.UAS.cUa/Scer\GAL4^NP5130 is a suppressor of enteroblast of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

bsk^DN.UAS.cUa, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Atg9^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Tang et al., 2013)

bsk^DN.UAS.cUa/Scer\GAL4²²¹ is a suppressor of microtubule | larval stage phenotype of Scer\GAL4²²¹, unc-104^GD13526

(Chen et al., 2012)

bsk^DN.UAS.cUa/Scer\GAL4²²¹ is a suppressor of larval dorsal multidendritic neuron ddaE | larval stage phenotype of Scer\GAL4²²¹, unc-104^GD13526

(Chen et al., 2012)

bsk^DN.UAS.cUa/Scer\GAL4²²¹ is a suppressor of dendrite | larval stage phenotype of Scer\GAL4²²¹, unc-104^GD13526

(Chen et al., 2012)

bsk^DN.UAS.cUa, Scer\GAL4²²¹ is a suppressor of microtubule | larval stage phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4²²¹

(Chen et al., 2012)

bsk^DN.UAS.cUa, Scer\GAL4²²¹ is a suppressor of larval dorsal multidendritic neuron ddaE | larval stage phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4²²¹

(Chen et al., 2012)

bsk^DN.UAS.cUa, Scer\GAL4²²¹ is a suppressor of dendrite | larval stage phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4²²¹

(Chen et al., 2012)

bsk^DN.UAS.cUa/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

NOT Suppressor of

Statement

Reference

bsk^DN.UAS.cUa, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing disc | larval stage phenotype of Nkap^GD11807, Scer\GAL4^Bx-MS1096

(Guo et al., 2024)

(Katheder et al., 2017)

(Katheder et al., 2017)

(Katheder et al., 2017)

Scer\GAL4^en.PU/bsk^DN.UAS.cUa is a non-suppressor of wing disc | third instar larval stage phenotype of Ipk2^20B

(Seeds et al., 2015)

Scer\GAL4^ap-md544/bsk^DN.UAS.cUa is a non-suppressor of adult thorax phenotype of Scer\GAL4^ap-md544, Tap42^GD8900

(Wang et al., 2012)

Other

Statement

Reference

Scer\GAL4^Act5C.PI, bsk^DN.UAS.cUa, scrib¹ has eye disc | somatic clone - tissue specific phenotype

(Kong et al., 2021)

Hsap\NCAM1^140.UAS, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has axon phenotype

(Bornstein et al., 2015)

Fas2^{UAS.ΔCT.KNSAV.Tag:FLAG}, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has axon phenotype

(Bornstein et al., 2015)

Fas1^+3.UAS, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has axon phenotype

(Bornstein et al., 2015)

Nrg^180.UAS, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has axon phenotype

(Bornstein et al., 2015)

Fas2^UAS.cLb, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has axon phenotype

(Bornstein et al., 2015)

Fas2^{UAS.PEST+.KNSAV.Tag:FLAG}, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has axon phenotype

(Bornstein et al., 2015)

Fas2^{UAS.PEST-.KNSAV.Tag:FLAG}, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has axon phenotype

(Bornstein et al., 2015)

Fas2^{UAS.CTshort.KNSAV.Tag:FLAG}, Scer\GAL4^Tab2-201Y, bsk^DN.UAS.cUa has axon phenotype

(Bornstein et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The additional clonal co-expression of bsk^{DN.Scer\UAS.cUa} suppresses the cell non-autonomous increase in autophagosomes (assessed by a Atg8a fluorescence reporter) in the midgut, but not in the somatic muscles or the fat body, of third instar larvae resulting from the presence of eye-antennal imaginal disc clones homozygous for scrib⁶⁷³ and expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU; the clonal co-expression of bsk^{DN.Scer\UAS.cUa} also suppresses the cell non-autonomous increase in autophagosomes in neighboring disc cells resulting from third instar larval eye-antennal imaginal disc clones co-expressing dlg1^GD4689 and Ras85D^V12.Scer\UAS under the control of Scer\GAL4^{Act5C.CoinFLP-FRT} (in combination with Dicer-2 for efficient RNAi).

(Katheder et al., 2017)

Both the frequent follicular epithelium multi-layering and the occasional invasion of germline cells by follicle cells exhibited by Pi3K59F^Δm22 homozygous follicle cell clones is strongly suppressed by the clonal expression of bsk^{DN.Scer\UAS.cUa} under the control of Scer\GAL4^tub.PU.

(O'Farrell et al., 2017)

The co-expression of bsk^DN.UAS.cUa suppresses both the increased numbers of intestinal stem cells/enteroblast and the increased mitotic index induced in the adult midgut epithelium by the adulthood-only expression of Uvrag^HMS01357 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts]).

(Nagy et al., 2016)

Co-expression of bsk^{DN.Scer\UAS.cUa} with Fas2^Scer\UAS.cLb (or Fas2^{Scer\UAS.PEST+.KNSAV.T:Zzzz\FLAG}, Fas2^{Scer\UAS.PEST-.KNSAV.T:Zzzz\FLAG}, Fas2^{Scer\UAS.CTshort.KNSAV.T:Zzzz\FLAG}, Fas2^{Scer\UAS.ΔCT.KNSAV.T:Zzzz\FLAG}, Fas1^+3.Scer\UAS, Nrg^180.Scer\UAS) by Scer\GAL4^Tab2-201Y results in mushroom body γ lobe axon pruning defects.

Co-expression of bsk^{DN.Scer\UAS.cUa} enhances axon-pruning defects in flies with Fas2^Scer\UAS.cLa driven by Scer\GAL4^Tab2-201Y.

Co-expression of bsk^{DN.Scer\UAS.cUa} with Fas2^{Scer\UAS.ΔCT.AAV.T:Zzzz\FLAG} (or Fas2^{Scer\UAS.PEST-.AAV.T:Zzzz\FLAG} or Fas3^Scer\UAS.cZa) by Scer\GAL4^Tab2-201Y does not result in mushroom body pruning defects.

(Bornstein et al., 2015)

Clonal co-expression of bsk^{DN.Scer\UAS.cUa} suppresses the invasiveness of clonal cells, but does not suppress tumor formation or developmental delay seen with eye-antennal disc clones co-expressing Ets21C^KK103211 and Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act.PU.

(Külshammer et al., 2015)

Expression of bsk^{DN.Scer\UAS.cUa} driven by Scer\GAL4^en.PU does not suppress increased cell death seen in Ipk2^20B/Ipk2^20B third instar larval wing discs.

(Seeds et al., 2015)

Expression of bsk^{DN.Scer\UAS.cUa} suppresses the rough eye phenotype seen when Atg9^{Scer\UAS.T:Zzzz\FLAG} is expressed under the control of Scer\GAL4^GMR.PU.

(Tang et al., 2013)

Co-expression of bsk^{DN.Scer\UAS.cUa} fails to rescue the cleft thorax phenotype of flies expressing Tap42^GD8900 under the control of Scer\GAL4^ap-md544.

(Wang et al., 2012)

Co-expression of bsk^{DN.Scer\UAS.cUa} ameliorates the eye defects caused by expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

(Griswold et al., 2008)

Xenogenetic Interactions

Statement

Reference

The aggressive tissue overgrowth observed in third instar larval wing discs carrying somatic (flip-out) clones simultaneously expressing Snr1^HMS00363 and BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^Act.PU is significantly suppressed by co-expression of bsk^{DN.Scer\UAS.cUa}.

(Xie et al., 2017)

Co-expression of bsk^{DN.Scer\UAS.cUa} with Hsap\NCAM1^140.Scer\UAS driven by Scer\GAL4^Tab2-201Y results in mushroom body γ lobe pruning defects.

(Bornstein et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Bsk^DN

(Nagy et al., 2016, Zhang et al., 2016)

bsk^{DN.Scer\UAS.cUa}

bsk^DN.UAS.cUa

bsk^DN

(Akbergenova and Littleton, 2017, Külshammer et al., 2015)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (55)

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