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General Information
Symbol
Dmel\bskDN.UAS.cUa
Species
D. melanogaster
Name
FlyBase ID
FBal0290703
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-bskDN, bskDN, bskDN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of a dominant-negative form of bsk.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The adulthood-only expression of bskDN.UAS.cUa under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) does not significantly affect the number of stem cells/enteroblasts in the adult midgut, despite decreasing the mitotic index of this epithelium, as compared to controls.
The expression of bskDN.Scer\UAS.cUa under the control of Scer\GAL4221 in ddaE neurons leads a defective early response to injury, as the pool of growing microtubules (identified as fluorescent EB1 comets) fails to show the expected reversal of polarity at 24h after laser-induced severance, as compared to controls.
Overexpression of bskDN.Scer\UAS.cUa in combination with Scer\GAL4Scer\UAS.cHa (but not expression of bskDN.Scer\UAS.cUa alone) driven by Scer\GAL4Tab2-201Y results in axon (but not dendrite) pruning defects.
ddaC neurons show robust dendrite regeneration after dendrite removal in animals expressing bskDN.Scer\UAS.cUa under the control of Scer\GAL4477. ddaE neurons show robust dendrite regeneration after dendrite removal in animals expressing bskDN.Scer\UAS.cUa under the control of Scer\GAL4221.
Expression of bskDN.Scer\UAS.cUa under the control of either Scer\GAL4esg-NP5130 or Scer\GAL4Myo31DF-NP0001 results in impaired intestinal stem cell proliferation in response to paraquat.
Larval ddaE neurons expressing bskDN.UAS.cUa under the control of Scer\GAL4221 (and Dicer-2, for a more efficient RNAi) show decreased growing microtubules within dendrites 8h after axotomy and show a decrease in the proportion of dendrites persisting after consecutive axotomy and dendrite severance (i.e. axotomy-8h-dendrite severance-18h), as compared to similarly injured control neurons; the former does not occur upon a 24h interval between axotomoy and dendrite severance.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Phenotype Manifest In
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
The additional clonal co-expression of bskDN.Scer\UAS.cUa suppresses the cell non-autonomous increase in autophagosomes (assessed by a Atg8a fluorescence reporter) in the midgut, but not in the somatic muscles or the fat body, of third instar larvae resulting from the presence of eye-antennal imaginal disc clones homozygous for scrib673 and expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU; the clonal co-expression of bskDN.Scer\UAS.cUa also suppresses the cell non-autonomous increase in autophagosomes in neighboring disc cells resulting from third instar larval eye-antennal imaginal disc clones co-expressing dlg1GD4689 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.CoinFLP-FRT (in combination with Dicer-2 for efficient RNAi).
Both the frequent follicular epithelium multi-layering and the occasional invasion of germline cells by follicle cells exhibited by Pi3K59FΔm22 homozygous follicle cell clones is strongly suppressed by the clonal expression of bskDN.Scer\UAS.cUa under the control of Scer\GAL4tub.PU.
The co-expression of bskDN.UAS.cUa suppresses both the increased numbers of intestinal stem cells/enteroblast and the increased mitotic index induced in the adult midgut epithelium by the adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts]).
Clonal co-expression of bskDN.Scer\UAS.cUa suppresses the invasiveness of clonal cells, but does not suppress tumor formation or developmental delay seen with eye-antennal disc clones co-expressing Ets21CKK103211 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU.
Expression of bskDN.Scer\UAS.cUa driven by Scer\GAL4en.PU does not suppress increased cell death seen in Ipk220B/Ipk220B third instar larval wing discs.
Expression of bskDN.Scer\UAS.cUa suppresses the rough eye phenotype seen when Atg9Scer\UAS.T:Zzzz\FLAG is expressed under the control of Scer\GAL4GMR.PU.
Co-expression of bskDN.Scer\UAS.cUa fails to rescue the cleft thorax phenotype of flies expressing Tap42GD8900 under the control of Scer\GAL4ap-md544.
Co-expression of bskDN.Scer\UAS.cUa ameliorates the eye defects caused by expression of Sir2Scer\UAS.cGa under the control of Scer\GAL4GMR.PU.
Xenogenetic Interactions
Statement
Reference
The aggressive tissue overgrowth observed in third instar larval wing discs carrying somatic (flip-out) clones simultaneously expressing Snr1HMS00363 and BacA\p35Scer\UAS.cHa driven by Scer\GAL4Act.PU is significantly suppressed by co-expression of bskDN.Scer\UAS.cUa.
Co-expression of bskDN.Scer\UAS.cUa with Hsap\NCAM1140.Scer\UAS driven by Scer\GAL4Tab2-201Y results in mushroom body γ lobe pruning defects.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Unknown
Secondary FlyBase IDs
    References (22)