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General Information
Symbol
Dmel\cpx1257
Species
D. melanogaster
Name
FlyBase ID
FBal0290886
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C4297556T

Amino acid change:

Q142term | cpx-PA; Q125term | cpx-PAA; Q139term | cpx-PC; Q142term | cpx-PG; Q142term | cpx-PH; Q142term | cpx-PI; Q83term | cpx-PJ; Q83term | cpx-PK; Q128term | cpx-PL; Q139term | cpx-PM; Q139term | cpx-PO; Q139term | cpx-PP; Q146term | cpx-PS; Q143term | cpx-PU; Q142term | cpx-PV; Q139term | cpx-PW; Q142term | cpx-PX; Q143term | cpx-PY

Reported amino acid change:

Q143term

Comment:

Nonsense mutation in the last codon of most cpx isoforms. This removes the last residue, which is a Q in the C-terminal CaaX motif, thought to be necessary for farnesylation and implicated in membrane targeting in certain mammalian isoforms. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nonsense mutation that removes the last C-terminal amino acid (a Q in the X position of the C-terminal CaaX sequence) encoded by an alternative exon (location 3R:123210..124307 , release 5 genome) that is present in most isoforms including cpx-RU (the exon is not present in the cpx-RE isoform).

Amino acid replacement: Q143term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

cpx1257/cpxSH1, but not cpx1257/+, larvae have significantly reduced locomotion, compared to controls; transheterozygotes also have significantly reduced numbers of synaptic vesicles around motor neuron T-bars and significantly increased NMJ presynaptic active zone number, but not area, compared to controls; they have significantly increased miniature excitatory postsynaptic currents (minis) and excitatory postsynaptic current (eEPSC) amplitude and decay time constant in muscle 6, compared to controls and paired-pulse depression of eEPSCs is significantly increased, compared to controls.

cpx1257/Df(3R)Exel6140 animals show a severe lack of motor coordination at 38[o]C.

The amplitude and waveform of the excitatory postsynaptic current (EPSC) at the dorsal longitudinal muscle neuromuscular synapse is not altered compared to wild type in cpx1257/Df(3R)Exel6140 adults. However, the mutant adults show an increase in miniature EPSC frequency compared to wild type. The same phenotypes are seen in recordings of cpx1257/Df(3R)Exel6140 larval neuromuscular junctions.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference

cpxSH1/cpx1257 has T-bar | larval stage phenotype, non-suppressible by brp69/brpnude

NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

brp69/+; cpx1257/+ double heterozygous larvae have significantly reduced locomotion, compared to controls, unlike either heterozygote alone.

brpnude/brp69; cpx1257/cpxSH1 double mutants have suppression of the increased eEPSC amplitude and mini frequency of cpx1257/cpxSH1 mutant larval muscle 6 and enhancement of the paired-pulse depression seen in both brpnude/brp69 mutants and cpx1257/cpxSH1 mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)