FlyBase Allele Report: Dmel\ref(2)P[UAS.Tag:HA]

General Information

Symbol

Dmel\ref(2)P^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0290912

Feature type

allele

Associated gene

Dmel\ref(2)P

Associated Insertion(s)

Carried in Construct

P{UAS-ref(2)P.HA}

Also Known As

UAS-ref(2)P

Key Links

Transgenic product class

wild_type

(de Castro et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(de Castro et al., 2013)

Mutagen

in vitro construct

(de Castro et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-ref(2)P.HA}

Cytology

Description

UASt regulatory sequences drive expression of ref(2)P which is tagged at the N-terminal end with Tag:HA.

(de Castro et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ref(2)P

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Cunningham et al., 2020)

modeled by Zzzz\GGGGCC^36.UAS

(Cunningham et al., 2020)

modeled by Zzzz\GGGGCC^{44.LDS.UAS.GR-GFP}

(Cunningham et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^{elav.Switch.PO}/ref(2)P^UAS.Tag:HA is a non-enhancer of short lived | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS

(Morón-Oset et al., 2023)

Suppressor of

Statement

Reference

ref(2)P^UAS.Tag:HA, Scer\GAL4^da.G32 is a suppressor of decreased rate of adult locomotory behavior phenotype of Cisd^UAS.cCa, Scer\GAL4^da.G32

(Martinez et al., 2024)

ref(2)P^UAS.Tag:HA/Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS

(Morón-Oset et al., 2023)

ref(2)P^UAS.Tag:HA/Scer\GAL4^GMR.PFa is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS

(Morón-Oset et al., 2023)

Scer\GAL4^Act.PU/ref(2)P^UAS.Tag:HA is a suppressor of visible phenotype of park²⁵

(de Castro et al., 2013)

ref(2)P^UAS.Tag:HA/Scer\GAL4^da.G32 is a suppressor | partially of visible phenotype of Pink1^B9

(de Castro et al., 2013)

Scer\GAL4^elav.PU/ref(2)P^UAS.Tag:HA is a suppressor | partially of visible phenotype of Pink1^B9

(de Castro et al., 2013)

ref(2)P^UAS.Tag:HA/Scer\GAL4^da.G32 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(de Castro et al., 2013)

Scer\GAL4^elav.PU/ref(2)P^UAS.Tag:HA is a suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(de Castro et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{elav.Switch.PO}/ref(2)P^UAS.Tag:HA is a non-suppressor of short lived | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS

(Morón-Oset et al., 2023)

Phenotype Manifest In

Suppressor of

Statement

Reference

ref(2)P^UAS.Tag:HA/Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS

(Morón-Oset et al., 2023)

ref(2)P^UAS.Tag:HA/Scer\GAL4^da.G32 is a suppressor | partially of adult thorax phenotype of Pink1^B9

(de Castro et al., 2013)

Scer\GAL4^Act.PU/ref(2)P^UAS.Tag:HA is a suppressor of adult thorax phenotype of park²⁵

(de Castro et al., 2013)

Scer\GAL4^elav.PU/ref(2)P^UAS.Tag:HA is a suppressor | partially of adult thorax phenotype of Pink1^B9

(de Castro et al., 2013)

ref(2)P^UAS.Tag:HA/Scer\GAL4^da.G32 is a suppressor | partially of indirect flight muscle cell phenotype of Pink1^B9

(de Castro et al., 2013)

ref(2)P^UAS.Tag:HA/Scer\GAL4^da.G32 is a suppressor | partially of mitochondrion phenotype of Pink1^B9

(de Castro et al., 2013)

Scer\GAL4^elav.PU/ref(2)P^UAS.Tag:HA is a suppressor of dopaminergic PPL1 neuron phenotype of Pink1^B9

(de Castro et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ref(2)P^{Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^da.G32 or Scer\GAL4^elav.PU significantly suppresses the penetrance of the indented thorax phenotype seen in Pink1^B9 mutants.

Expression of ref(2)P^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 suppresses the reduced climbing ability of Pink1^B9 adults. Indirect flight muscles show improved tissue structure and mitochondrial integrity compared to Pink1^B9 single mutants.

Expression of ref(2)P^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PU suppresses the loss of dopaminergic neurons in the PPL1 cluster which is seen in Pink1^B9 mutants.

The ability of ref(2)P^{Scer\UAS.T:Ivir\HA1} expressed under the control of Scer\GAL4^da.G32 to suppress the Pink1^B9 mutant phenotypes of an indented thorax and reduced climbing ability is suppressed if the flies are also mutant for Atg1^Δ3D.

Expression of ref(2)P^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU does not suppress the indented thorax phenotype seen in park²⁵ flies.

(de Castro et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ref(2)P^UAS.Tag:HA/Scer\GAL4^VP16.bam rescues ref(2)P^od3

(de Castro et al., 2013)

Comments

Expression of ref(2)P^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^{bam.T:Hsim\VP16} rescues the sterility of ref(2)P^od3 males.

(de Castro et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ref(2)P^{Scer\UAS.T:Ivir\HA1}

ref(2)P^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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