FB2025_01 , released February 20, 2025
Allele: Ecol\lexAp65.nompC
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General Information
Symbol
Ecol\lexAp65.nompC
Species
E. coli
Name
FlyBase ID
FBal0291604
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
Carried in Construct
PBac{nompC-lexA::p65}
Also Known As
nompC-LexA, nompC-LexAp65, nompC-LexA::p65
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Shearin et al., 2013)
Progenitor genotype
Carried in construct
PBac{nompC-lexA::p65}
Cytology
Description

nompC regulatory sequences control expression of a lexA::p65 driver. 3'UTR sequences from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene are present downstream of the coding sequence.

(Shearin et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
nompC
Encoded product / tool
lexA::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval multidendritic class III neuron
      (Burgos et al., 2018, Shearin et al., 2013)
      sensory neuron | subset
      (Otto et al., 2018)
      chordotonal neuron
      (Shearin et al., 2013)
      vch1 neuron
      (Shearin et al., 2013)
      vch2 neuron
      (Shearin et al., 2013)
      lch1 neuron
      (Shearin et al., 2013)
      larval abdominal lch5 neuron
      (Shearin et al., 2013)
      adult stage
      mechanosensory neuron of labellar taste peg
      (Sánchez-Alcañiz et al., 2018)
      mechanosensory neuron
      (Li and Montell, 2021)
      labellar taste peg mechanosensory neuron
      (Li and Montell, 2021)
      Additional Information
      Statement
      Reference

      EcollexAp65.nompC drives expression in a subset of sensory neurons that project to the ventral side of the larval neuropil.

      (Otto et al., 2018)

      Drives expression in mechanosensory neurons, including larval multidendritic class III neurons and labellar taste peg mechanosensory neurons.

      (FlyBase Curators, 2017)

      EcollexAp65.nompC drives expression in larval multidendritic class III neurons and in larval scolopidial (chordotonal) neurons.

      (Shearin et al., 2013)
       
      Marker for
      larval multidendritic class III neuron
      (Burgos et al., 2018)
      Reflects expression of
      nompC
      (Li and Montell, 2021, Burgos et al., 2018, Otto et al., 2018, Sánchez-Alcañiz et al., 2018, Shearin et al., 2013)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2lexAop.cHa) of Ecol\lexAp65.nompC cells in adult females does not affect preference for softer plain, or 100 mM sucrose-containing, agarose for egg laying, compared to controls.

      (Wu et al., 2019)

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus or Cnoc\ChR1::Csub\ChRlexAop.FRT.CsChrimson.Venus with 617 nm (red) light) of Ecol\lexAp65.nompC cells in adults induces labellar spread, compared to controls; there is also an induction of proboscis retraction due to stimulation during feeding, compared to controls; when activation is restricted to a subset of Ecol\lexAp65.nompC cells (using Scer\GAL4NP7506 and Scer\FLP1UAS.cUa with Cnoc\ChR1::Csub\ChRlexAop.FRT.CsChrimson.Venus), there is an induction of labellar spread, but not proboscis retraction during feeding, compared to controls; activation of this subset in the labellum also results in activation of one pair of Scer\GAL4GMR18B07-expressing motor neurons in the brain with a 2 ms delay, compared to baseline.

      (Zhou et al., 2019)

      Inhibition (using Ctet\tetXTNT-LC.13x.lexAop.Tag:HA) of Ecol\lexAp65.nompC cells in starved adult males significantly reduces preference for softer sucrose-containing agarose, compared to controls.

      (Sánchez-Alcañiz et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lexAnompC.T:Hsap\RELA
      Ecol\lexAp65.nompC
      Name Synonyms
      Secondary FlyBase IDs
        References (21)