eye, with Scer\GAL4GMR.PF
Expression of Nek2Scer\UAS.T:Avic\GFP in the peripodial cells of the developing wing epithelium under the control of Scer\GAL4ptc-559.1 leads to an increase in the number of centrosomes. The entire wing disc is significantly altered in size and shape. The adult wing vein pattern is also disrupted.
Expression of Nek2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4pnr.PU leads to incomplete closure of the notum, abnormal patterning of the bristles and a severe defect in the scutellum.
Expression of Nek2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PF leads to a rough eye phenotype in the adult. Extensive mispatterning and positioning of the ommatidia is seen.
Expression of Nek2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PF results in a "distant seeding" phenotype; Avic\GFP spots are detected in other parts of adult flies, including the notum, wing and legs. This phenotype is strongly suppressed when the flies are fed the drugs neratinib and pelitinib. No effect is seen with lapatinib.
Nek2UAS.GFP is an enhancer of visible | adult stage | somatic clone phenotype of RetMEN2B.UAS
Nek2UAS.GFP, RetMEN2B.UAS, Scer\GAL4ptc-559.1 has abnormal cell migration phenotype
Nek2UAS.GFP is an enhancer of eye | somatic clone phenotype of RetMEN2B.UAS
Nek2UAS.GFP is an enhancer of eye disc | larval stage | somatic clone phenotype of Cskunspecified, Ras85DV12.UAS
Nek2UAS.GFP, RetMEN2B.UAS, Scer\GAL4ptc-559.1 has wing disc phenotype
Nek2UAS.GFP, RetMEN2B.UAS has proboscis | somatic clone phenotype
Expression of Nek2Scer\UAS.T:Avic\GFP enhances the rough eye phenotype seen in eye clones expressing RetMEN2B.Scer\UAS, emergence of unpigmented, undifferentiated cells in the adult eye and the presence of clonal cells in tissues adjacent to the eye, including the proboscis.
Co-expression of RetMEN2B.Scer\UAS and Nek2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4ptc-559.1 results in a large number of cells migrating away from the A-P boundary into the adjacent compartment. Extensive rearrangement of the cytoskeletal tubulin network is also seen.
Expression of Nek2Scer\UAS.T:Avic\GFP enhances the tissue overgrowth seen in larval eye disc clones expressing Cskunspecified and Ras85DV12.Scer\UAS, and leads to the production of secondary tumours in the body of the larvae. This phenotype can be suppressed by feeding flies with the Akt1 inhibitor MZ2206; significantly smaller primary tumours are seen and there is a complete absence of secondary tumours.
Injection of dissociated cells from Nek2Scer\UAS.T:Avic\GFP Cskunspecified Ras85DV12.Scer\UAS mutant eye discs (generated using ey-FLP MARCM) into the dorsal notum region of wild type adult flies results in detection of T:Avic\GFP positive cells in various parts of the adult body. Dispersed Avic\GFP-positive cells are not seen when flies are injected with eye disc cells that express Nek2Scer\UAS.T:Avic\GFP alone.
Expression of TordsRNA.Scer\UAS.cUa suppresses the "distant seeding" phenotype seen when Nek2Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4GMR.PF.
Expression of RetMEN2B.Scer\UAS enhances the "distant seeding" phenotype seen when Nek2Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4GMR.PF. Larger groups of Avic\GFP-positive cells are detected in distant body parts. This phenotype is significantly reduced when flies also carry one copy each of Pi3K21Bunspecified and S6kunspecified. The phenotype can also be suppressed by drugs that inhibit signalling along the PI3K cascade; LY294002, Rapamycin, MK2206 or BEZ235.
